Yersinia Pestis Protocols

Specificaties
Gebonden, blz. | Engels
Springer Nature Singapore | e druk, 2018
ISBN13: 9789811079467
Rubricering
Springer Nature Singapore e druk, 2018 9789811079467
Onderdeel van serie Springer Protocols Handbooks
Verwachte levertijd ongeveer 9 werkdagen

Samenvatting

This protocol book includes common and advanced research protocols for Yersinia pestis research and for its identification, genotyping and source-tracing. This book includes protocols for genomic and transcriptomic analysis, small RNA study, protein-protein interaction, gene deletion. It will be helpful for graduate students, clinicians, and researchers in infectious diseases, systems biology, and evolution and numerous other relevant fields.

Specificaties

ISBN13:9789811079467
Taal:Engels
Bindwijze:gebonden
Uitgever:Springer Nature Singapore

Inhoudsopgave

<div>Isolation and identification of Yersinia pestis.- Isolation and characterization of Yersinia pestis bacteriophages.- Extraction of chromosomal DNA and plasmid from Yersinia pestis.- Extraction of total RNAs from Yersinia pestis.- Genotyping of Yersinia pestis.- Genome-wide variation analysis of Yersinia pestis.- Genome-wide detection of expressed sRNAs in Y. pestis with RNA-seq.- Validation of Y. pestis sRNAs with nonradiative-labeled Northern Blot.- Determination of transcriptional start sites (TSSs) in Y. pestis with a primer extension assay.- Purification of Hfq-associated RNAs with RNA immunoprecipitation (RIP).- Overexpression of target sRNA in tightly controlled multicopy plasmids.- Target validation of sRNA with a GFP reporter gene fusion system.- Inducible gene expression in Yersinia pestis.- Analysis of Yops secretion by Y. pestis Type III secretion system.- Co-Immunoprecipitation analysisfor detection of protein-protein interaction in Y. pestis.- Detection of type III secretion system needle assembly in Yersinia pestis by cross-linking of YscF.- Evaluation of protein translocation into eukaryotic cells by Y. pestis.- Adhesion analysis of Y. pestis to the host cells.- Construction of gene deletion mutants in Yersinia pestis.- CRISPR-Cas12a–assisted recombineering in Yersinia pestis.- Plasmid curing in Yersinia pestis.- Genetic regulation analysis of Yersinia pestis.- Investigation of Biofilm formation in Y. pestis.- Extraction and purification of F1 capsule antigen from Y. pestis.- Screening of regulator responses to Yersinia pestis infection with a concatenated tandem array of transcription factor response elements-pull down.- Animal models for Yersinia pestis.- Antibiotic resistance evaluation of Yersinia pestis.- PCR for detection and identification of Yersinia pestis.- Immunological methods for detection and identification of Yersinia pestis.- Analysis of fatty acids in Yersinia pestis by gas chromatography.- Evaluation of efficacy of Yersinia pestis  vaccine.- Online resources for Yersinia pestis.- Effects of Y. pestis on macrophage.</div>

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        Yersinia Pestis Protocols