Assessment of Mammalian Embryo Quality

List of contributors. Preface. Introduction: Only the best conceptus will do! But what does best mean? M.H. Johnson. 1. Human embryo morphology and developmental capacity; M. Alikani, et al. 2. Morphological evaluation of embryos in domestic species; S. Merton. 3. Metabolic markers of embryo viability; I. Donnay. 8. Biopsying and genotyping cattle embryos; E. Mullaart. 9. Multiphoton microscopy for imaging mammalian embryos; J.M. Squirrell. 10. Is the frequency of chromosome abnormalities influenced by in vitro techniques? D. Viuff, et al. 11. Differential staining of inner and outer cells to assess mammalian embryo quality; A. Van Soom, et al. 12. Apoptosis in mammalian embryos; K. Hardy, S. Spanos. 13. Electron microscopy of pre-hatching mammalian embryos: the ultra structure of cattle, swine and horse embryos; P. Maddox-Hyttell , M. Boerjan. 14. Differences in gene expression patterns related to origin of pre-implantation embryos; C.Wrenzycki, H. Niemann. 15. Protocol. Appendix I: The electron microscope; P. Maddox-Hyttel. Appendix II: Processing of eggs for transmission electron microscopy; P. Maddox-Hyttel. Appendix III: Human pre-implantation diagnosis pcr and RT-PCR; D. Taylor. Appendix IV: Human pre-implantation diagnosis fluorescence in site-hybridization (FISH) on individual blazoners; D. Taylor. Appendix V: Fluorescence in situ-hybridization (FISH) on metaphases in bovine embryos; D. Viuff. Appendix VI: Differential staining; A. Van Soom. Index. Colour plates.