CE in Biotechnology: Practical Applications for Protein and Peptide Analyses

1 Capillary Electrophoresis in the Development of Recombinant Protein Biopharmaceuticals.- 1.1 Summary.- 1.2 Introduction.- 1.3 Capillary Electrophoresis Reviews.- 1.4 Reviews of CE Used for the Analysis of Recombinant Proteins.- 1.5 Recent CE Literature.- 1.6 Quality Control and Validation.- 1.7 Developments in CE-MS.- 1.8 References.- 2 Selection of Buffers in Capillary Zone Electrophoresis: Application to Peptide and Protein Analysis.- 2.1 Introduction.- 2.2 Buffer Parameters.- 2.3 Buffers Used for the Separation of Peptides and Proteins.- 2.4 Acknowledgements.- 2.5 References.- 3 Reverse-Phase High Performance Liquid Chromatography and Capillary Zone Electrophoresis Separation of Protegrin Analogs.- 3.1 Abstract.- 3.2 Introduction.- 3.3 Experimental.- 3.4 Results and Discussion.- 3.5 References.- 4 Use of Capillary Electrophoresis to Determine the Dilute Protein Concentration in Formulations Containing Interfering Excipients.- 4.1 Abstract.- 4.2 Introduction.- 4.3 Material and Methods.- 4.4 Result and Discussion.- 4.5 Conclusion.- 4.6 Acknowledgements.- 4.7 References.- 5 Effects of the Solution Environment on the Resolution of Recombinant Human Deoxyribonuclease Variants in Capillary Zone Electrophoresis.- 5.1 Abstract.- 5.2 Introduction.- 5.3 Materials.- 5.4 Methods.- 5.5 Results and Discussion.- 5.6 Conclusion.- 5.7 Acknowledgements.- 5.8 References.- 6 Recent Advances in Capillary Isoelectric Focusing.- 6.1 Introduction.- 6.2 Theory and Practice of cIEF.- 6.3 cIEF in Microchannels.- 6.4 Imaging cIEF.- 6.5 cIEF-Mass-Spectrometry.- 6.6 Capillary Isoelectric Focusing of Monoclonal Antibodies.- 6.7 Capillary Isoelectric Focusing of Hemoglobin and Hemoglobin Variants.- 6.8 Capillary Isoelectric Focusing of Erythropoietin.- 6.9 Novel Applications of cIEF.- 6.10 References.- 7 Separation of Enbrel® (rhuTNFR: Fc) Isoforms by Capillary Isoelectric Focusing.- 7.1 Abstract.- 7.2 Introduction.- 7.3 Experimental.- 7.4 Results and Discussion.- 7.5 Acknowledgements.- 7.6 References.- 8 Optimization, Validation, and Use of Capillary Gel Electrophoresis for Quality Control Testing of Synagis®, a Monoclonal Antibody.- 8.1 Abstract.- 8.2 Introduction.- 8.3 Methods.- 8.4 Regulatory Guidance.- 8.5 Optimization Strategy.- 8.6 Validation.- 8.7 Validation Parameters for CGE.- 8.8 Acknowledgements.- 8.9 References.- 9 Analysis of Protein Therapeutics by Capillary Electrophoresis.- 9.1 Introduction.- 9.2 Capillary Electrophoresis — Sodium Dodecylsulfate (CE-SDS).- 9.3 Capillary Isoelectric Focusing (cIEF).- 9.4 Capillary Zone Electrophoresis (CZE).- 9.5 Carbohydrate Analysis.- 9.6 Methods.- 9.7 Acknowledgements.- 9.8 References.- 10 Sensitive and High Resolution CE/MS/MS for Protein Identification in Complex Mixtures.- 10.1 Introduction.- 10.2 Approaches to Improve the Sensitivity of CE/MS/MS for Protein Identification.- 10.3 Approaches to Improve the Resolution of CE/MS/MS for Protein Identification.- 10.4 Other Approaches.- 10.5 Conclusions.- 10.6 References.- Abbreviations.