<p>Contents</p><p> </p><p>Preface</p><p>Contributors</p><p> </p><p>Part I Fixation, Processing, Embedding and Staining of Botanical Specimens </p><p> </p><p>1. A Guide to the Study of Plant Structure with Emphasis on Living Specimens</p><p>Edward C. Yeung </p><p> </p><p>2. Chemical and Physical Fixation of Cells and Tissues: an Overview</p><p>Bing Q. Huang and Edward C. Yeung </p><p> </p><p>3. Paraffin and Polyester Waxes</p><p>Claudio Stasolla and Edward C. Yeung </p><p> </p><p>4. The Glycol Methacrylate Embedding Resins – Technovit 7100 and 8100</p><p>Edward C. Yeung and Colin K. W. Chan</p> <p></p><p>5. Epoxy Resins for Light and Transmission Electron Microscopy</p><p>Michael J. Sumner</p><p> </p><p>6. LR White Acrylic Resin</p><p>Edward C. Yeung and Bing Q. Huang</p><p> </p><p>7. High Pressure Freezing and Freeze Substitution of In vivo and In vitro Cultured Plant Samples</p><p>Jose M. Seguí-Simarro</p><p> </p><p>8. Three-Dimensional Imaging for Electron Microscopy of Plastic-embedded Plant Specimens</p><p>Jose M. Seguí-Simarro</p><p> </p><p>9. Fluorescent Staining of Living Plant Cells </p><p>Sarah Schoor, Shiu-Cheung Lung, Dustin Sigurdson, and Simon D. X. Chuong</p> <p></p><p>10. Improved Methods for Clearing and Staining of Plant Samples</p><p>Alexander Lux, Marek Vaculík, and Ján Kováč</p><p> </p><p>Part II Microtechniques related to Cell and Molecular biology studies</p><p> </p><p>11. Whole Mount Immunofluorescence Staining of Plant Cells and Tissues </p><p>Masaki Shimamura </p><p>12. Protoplast Isolation and Staining</p><p>Shiu-Cheung Lung, Sarah Schoor, Dustin Sigurdson, Makoto Yanagisawa, Kelly Yeung, Mi Qi Liu and Simon D. X. Chuong</p><p> </p><p>13. Guiding Principles for Live Cell Imaging of Plants using Confocal Microscopy</p><p>Subramanian Sankaranarayanan and Marcus A. Samuel</p> <p></p><p>14. Immunogold Labeling for Electron Microscopy: Strategy and Problem Solving</p><p>Fengli Guo and Bing Q. Huang</p><p> </p><p>15. Abscisic Acid Immunostaining</p><p>Yung-I Lee and Mei-Chu Chung</p><p> </p><p>16. Plant Chromosome Preparations and Staining for Light Microscopic Studies</p><p>Subhash C. Hiremath and C.C. Chinnappa </p><p> </p><p>17. Chromosome techniques and FISH</p><p>Mei-Chu Chung</p><p> </p><p>18. Detection of S-phase of Cell Division Cycle in Plant Cells and Tissues by</p> using 5-ethynyl-2’-deoxyuridine (EdU)<p></p><p>Soujanya Kuntam and Ferhan Ayaydin</p><p> </p><p>19. Staining Methods for Programmed Cell Death</p><p>Owen S. D. Wally and Claudio Stasolla</p><p> </p><p>20. Laser Microdissection of Plant Tissues </p><p>Jenna L. Millar, Michael G. Becker, and Mark F. Belmonte</p><p> </p><p>21. RNA In situ Hybridization</p><p>Shalini Mukherjee, Claudio Stasolla, and Belay Ayele</p><p> </p><p>Part III Preparative Methods for the Study of Wood Anatomy</p><p> </p><p>22. Microscopic Examination of Wood: Sample Preparation and Techniques for Light Microscopy</p><p>Jacques</p> C. Tardif and France Conciatori<p></p><p> </p><p>23. Collecting and Processing Wood Microcores for Monitoring Xylogenesis</p>Annie Deslauriers, Sergio Rossi, and Eryuan Liang<p> </p><p>24. Three-dimensional Imaging of Cambium and Secondary Xylem Cells by Confocal Laser Scanning Microscopy</p><p>Satoshi Nakaba, Peter Kitin, Yusuke Yamagishi, Shahanara Begum, Kayo Kudo, Widyanto Dwi Nugroho, and Ryo Funada</p><p> </p><p>Part IV Botanical Techniques and Protocols for Archeology and Herbarium Collection</p><p> </p><p>25. Collection of Plant Remains from Archaeological Contexts</p><p>Alessandra Celant, Donatella Magri, and Francesca Romana Stasolla</p><p> </p><p>26. </p>Archaeological Wood Preparation <p></p><p>Alessandra Celant and Gemma Coccolini</p><p> </p><p>27. Archaeopalynological Preparation Techniques </p><p>Donatella Magri and Federico Di Rita</p><p> </p><p>28. Phytoliths: Preparation and Archaeological Extraction </p><p>Brian Kooyman</p><p> </p><p>29. Starch Granules: Preparation and Archaeological Extraction </p><p>Brian Kooyman </p><p> </p><p>30. Plant Collection, Identification, and Herbarium Procedures</p><p>Bonnie Smith and C. C. Chinnappa</p><p> </p><p> </p><p>Index </p><p> </p><p></p> </p>
