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Plant Chemical Genomics

Methods and Protocols

Specificaties
Gebonden, 269 blz. | Engels
Humana Press | 2014e druk, 2013
ISBN13: 9781627035910
Rubricering
Humana Press 2014e druk, 2013 9781627035910
Onderdeel van serie Methods in Molecular Biology
Verwachte levertijd ongeveer 9 werkdagen

Samenvatting

Plant Chemical Genomics: Methods and Protocols , expert researchers in the field detail many of the techniques used for identification of bioactive compounds from a large selection of fields in plant biology including plant pathogenesis, immune responses, small RNA processing, endomembrane trafficking, lipids, plant hormone signaling and cell wall. The presentation of these and other examples as well as synthetic chemistry, computation and target identification provides a comprehensive overview of the practical aspects of chemical biology that are possible in plant systems. Small molecules and natural products that produce a phenotype in plants have been known for decades. The agrichemical industry has utilized small molecules based on known natural molecules such as auxins, cytokinins, abscissic acid and other growth regulators. In recent years, the screening and characterization of novel small molecules has enhanced our understanding of protein function, metabolic, signaling and endomembrane pathways, and their interactions in basic plant research.

Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Specificaties

ISBN13:9781627035910
Taal:Engels
Bindwijze:gebonden
Aantal pagina's:269
Uitgever:Humana Press
Druk:2014

Inhoudsopgave

<p>Section I: Automation and Imaging</p><p>1. Fully-automated Compound Screening in Arabidopsis thaliana Seedlings</p><p>Dominique Audenaert, Long Nguyen, Bert De Rybel, and Tom Beeckman</p><p> </p><p>2. Time-profiling Fluorescent Reporters in the Arabidopsis Root</p><p>Antoine P. Larrieu, Andrew P. French, Tony P. Pridmore, Malcolm J. Bennett and Darren M. Wells</p><p> </p><p>3. Screening for Bioactive Small Molecules by in vivo Monitoring of Luciferase-based Reporter Gene Expression in Arabidopsis thaliana </p><p>Christian Meesters and Erich Kombrink </p><p> </p><p> </p><p>Section II: Chemical Screening</p><p>4. Application of Yeast-two Hybrid Assay to Chemical Genomic Screens: A High-throughput System to Identify Novel Molecules Modulating Plant Hormone Receptor Complexes</p><p>Andrea Chini</p><p> </p><p>5. High-throughput Screening of Small Molecule Libraries for Inducers of Plant Defense Responses</p><p>Colleen Knoth and Thomas Eulgem<sup></sup></p><p> </p><p>6. Using a Reverse Genetics Approach to Investigate Small Molecule Activity</p><p>Siamsa M. Doyle and  Stéphanie Robert</p><p> </p><p>7. Investigating the Phytohormone Ethylene Response Pathway by Chemical Genetics </p><p>Lee-Chung, Lin, Chiao-Mei Chueh, and Long-Chi Wang</p><p> </p><p>8. Screening for Inhibitors of Chloroplast Galactolipid Synthesis Acting in membrano and in Planta</p><p>Laurence Boudière and Eric Maréchal</p><p> </p><p>9. Forward Chemical Screening of Small RNA Pathways</p><p>Yifan Lii and Hailing Jin</p><p> </p><p>10. Identification and Use of Fluorescent Dyes for Plant Cell Wall Imaging Using High-Throughput Screening</p><p>Charles T. Anderson and Andrew Carroll</p><p> </p><p>11. High-throughput Identification of Chemical Endomembrane Cycling Disruptors Utilizing Tobacco Pollen</p><p>Michelle Q. Brown, Nolan Ung, Natasha V. Raikhel, and Glenn R. Hicks</p><p> </p><p>12. Plant Chemical Genomics: Gravity Sensing and Response</p><p>Marci Surpin</p> 13. Screening Chemical Libraries for Compounds that Affect Protein Sorting to the Yeast Vacuole<p>Jan Zouhar</p><p> </p><p>Section III: Cheminformatics</p><p>14. The use of Multi-drug Approach to Uncover New Players of the Endomembrane System Trafficking Machinery</p><p>Urbina D, Pérez-Henríquez P,  and Norambuena L</p> 15. Cheminformatic Analysis of High-Throughput Compound Screens<p>Tyler W. H. Backman and Thomas Girke</p><p> </p><p>16. Endomembrane Dissection Using Chemically-Induced Bioactive Clusters</p><p>Natasha Worden, Thomas Girke, and Georgia Drakakaki<sup></sup></p> 17. Statistical Molecular Design: A Tool to Follow Up Hits From Small Molecule ScreeningAnders E. G. Lindgren, Andreas Larsson, Anna Linusson, and Mikael Elofsson<p> </p><p> </p><p>Section IV: Target Identification</p><p>18. Early Stage Hit Triage for Plant Chemical Genetic Screens and Target Site Identification</p><p>Terence A. Walsh</p><p> </p><p>19. Screening for Gene Function using the FOX (Full-length cDNA OvereXpressor gene) Hunting System</p><p>Mieko Higuchi, Minami Matsui</p><p> </p><p> </p><p> </p><p>Section V: Hormone Transport and Metabolite Profiling</p><p>20. Quantification of Stable Isotope Label in Metabolites via Mass Spectrometry</p><p>Jan Huege, Jan Goetze, Frederik Dethloff, Bjoern Junker, and Joachim Kopka<sup></sup></p><p> </p><p>21. <sup>1</sup>H NMR Based Metabolomics Methods for Chemical Genomics Experiments</p><p>Daniel J. Orr, Gregory A. Barding, Jr., Christiana E. Merrywell, Glenn R. Hicks, Natasha V. Raikhel, and Cynthia K. Larive</p><p> </p><p>22. Determination of Auxin Transport Parameters on the Cellular Level</p><p>Jan Petrášek, Martina Laňková, and Eva Zažímalová</p><p> </p><p>23. Analyzing the in vivo Status of Exogenously Applied Auxins: A HPLC-based Method to Characterize the Intracellularly Localized Auxin Transporters</p><p>Sibu Simon, Petr Skůpa, Petre I. Dobrev, Jan Petrášek, Eva Zažímalová, and Jiří Friml<sup></sup></p>

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        Plant Chemical Genomics