Mucins

<p>Mucin Methods: Genes Encoding Mucins And Their Genetic Variation With A Focus On Gel Forming Mucins.- Gel-forming and Cell-associated Mucins – preparation for Structural and Functional Studies.- Detecting, Visualising and Quantifying Mucins.- Mass Spectrometric Analysis of Mucin Core Proteins.- O-Glycoprotein Biosynthesis:  Site Localization by Edman Degradation and Site Prediction Based on Random Peptide Substrates.- Analysis of Assembly of Secreted Mucins.- MUC1 Membrane Trafficking: Protocols for Assessing Biosynthetic Delivery, Endocytosis, Recycling and Release Through Exosomes.- Glycomic Work-flow for Analysis of Mucin O-linked Oligosaccharides.- Glycomic Work-flow for Analysis of Mucin O-linked Oligosaccharides.- O-Glycomics: Profiling and Structural Analysis of Mucin-type O-linked Glycans.- O-Glycoproteomics: Site-specific O-glycoprotein Analysis by CID/ETD Electrospray Ionization Tandem Mass Spectrometry and Top-Down Glycoprotein Sequencing by In-Source Decay MALDI Mass Spectrometry.- Analysing Mucin Degradation.- Assessment of Mucus Thickness and Production In Situ.- Preservation of Mucus in Histological Sections, Immunostaining of Mucins in Fixed Tissue and Localization of Bacteria with FISH.- In vitro Measurements of Mucus Secretion by Colon Explants.- Establishment of Respiratory Air-Liquid Interface Cultures and their Use in Studying Mucin Production, Secretion, and Function.- Studying Mucin Secretion from Human Bronchial Epithelial Cell Primary Cultures.- Assessment of Intracellular Mucin Content In Vivo.- Techniques for Assessment of Interactions of Mucins with Microbes and Parasites In Vitroand In Vivo.- Assessing Mucin Expression and Function in Human Ocular Surface Epithelia In Vivo and In Vitro.</p>