Plant Genomics
Methods and Protocols
Samenvatting
Plant genomics is a growing and constantly evolving field of study, one which has gained much ground in past years through the development of advanced research and data management tools. In Plant Genomics: Methods and Protocols, expert researchers explore the current issues and methodologies of this expanding field, specifically addressing areas of gene discovery and the functional analysis of genes with a focus on the primary tools and sub-disciplines of genetic mapping, mRNA, protein and metabolite profiling. Chapters employ exciting new methods to investigate molecular plant breeding technology and gene functional analysis via transformation, mutation, protein function, and gene expression. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Comprehensive and innovative, Plant Genomics: Methods and Protocols is an essential guide for all plant scientists who are interested in further studies in the area of genomics research.
Specificaties
Inhoudsopgave
Daryl J. Somers, Peter Langridge, and J. P. Gustafson, eds.
Table of Contents
Preface
Contributors
1. Role of Model Plant Species
Richard Flavell
2. New Technologies for Ultra-High Throughput Genotyping in Plants
Nikki Appleby; David Edwards; Jacqueline Batley
3. Genetic Maps and the Use of Synteny
Chris Duran; David Edwards; Jacqueline Batley
4. A Simple TAE-based Method to Generate Large Insert BAC Libraries from Plant Species
Bu-Jun Shi; J. Perry Gustafson; Peter Langridge
5. Transcript Profiling and Expression Level Mapping
Elena Potokina; Arnis Druka; Michael J. Kearsey
6. Methods for Functional Proteomic Analyses
Christof Rampitsch; Natalia V. Bykova
7. Stable Transformation of Plants
Huw D. Jones; Caroline A. Sparks
8. Transient Transformation of Plants
Huw D. Jones; Angela Doherty; Caroline A. Sparks
9. Bridging the Gene-to-Function Knowledge Gap Through Functional Genomics
Stephen J. Robinson; Isobel A. P. Parkin
10. Heterologous and Cell Free Protein Expression Systems
Naser Farrokhi; Maria Hrmova; Rachel A. Burton; Geoffrey B. Fincher
11. Functional Genomics and Structural Biology in the Definition of Gene Function
Maria Hrmova; Geoffrey B. Fincher
12. In situ Analysis of Gene Expression in Plants
Sinéad Drea; Paul Derbyshire; Rachil Koumproglou; Liam Dolan; John H. Doonan; Peter Shaw
13. Plant and Crop Databases
David E. Matthews; Gerard R. Lazo; Olin D. Anderson
14. Plant Genome Annotation Methods
Shu Ouyang; Françoise Thibaud-Nissen; Kevin L. Childs; Wei Zhu; C. Robin Buell
15. Molecular Plant Breeding:Methodology and Achievements
Rajeev K. Varshney; Dave A. Hoisington; Spurthi N. Nayak; Andreas Graner
16. Practical Delivery of Genes to the Marketplace
David A. Fischhoff; Molly N. Cline
17. Ecological Genomics of Natural Plant Populations: The Israeli Perspective
Eviatar Nevo
18. Genome Sequencing Approaches and Successes
Michael Imelfort; Jacqueline Batley; Sean Grimmond; David Edwards