Ezio Rosato
Humana Press
0e druk, 2010
9781617375538
Circadian Rhythms
Methods and Protocols
Specificaties
Paperback, 578 blz.
|
Engels
Humana Press |
0e druk, 2010
ISBN13: 9781617375538
Rubricering
Onderdeel van serie
Methods in Molecular Biology
Verwachte levertijd ongeveer 8 werkdagen
Samenvatting
This is an in-depth examination of circadian biology, presented by leading researchers in the field. Methods for analysis of rhythmic readouts in select model organisms are included. This cutting-edge collection of protocols is adaptable for research at every level, and represents the huge strides that chronobiologists have made over the past two decades. Circadian biologists at all research levels will realize tremendous benefit from this extraordinary collection.
Specificaties
ISBN13:9781617375538
Taal:Engels
Bindwijze:paperback
Aantal pagina's:578
Uitgever:Humana Press
Druk:0
Inhoudsopgave
Preface
Contributors
I. Overviews.
1. Light, photoreceptors and circadian clocks
Russell G. Foster, Mark W. Hankins and Stuart N. Peirson
2. Statistical analysis of biological rhythm data
Harold B. Dowse
II. Rhythmic Readouts.
3. Rhythmic conidiation in Neurospora crassa.
Cas Kramer
4. Monitoring and analyzing Drosophila circadian locomotor activity.
Mauro A. Zordan, Clara Benna and Gabriella Mazzotta.
5. Automated video image analysis of larval zebrafish locomotor rhythms.
Gregory M. Cahill
6. Locomotor activity in rodents.
Gianluca Tosini
7. Analysis of circadian leaf movement rhythms in Arabidopsis thaliana.
Kieron D. Edwards and Andrew J. Millar
8. Detection of rhythmic bioluminescence from luciferase reporters in cyanobacteria.
Shannon R. Canales, Jayna L. Ditty, Eugenia M. Clerico and Susan S. Golden
9. Analysis of rhythmic gene expression in adult Drosophila using the firefly-luciferase reporter gene.
Ralf Stanewsky
10. Monitoring circadian rhythms in Arabidopsis thaliana using luciferase reporter genes.
Anthony Hall and Paul Brown
III. Identification of Clock Genes.
11. Specialized techniques for site directed mutagenesis in cyanobacteria
Eugenia M. Clerico, Jayna L. Ditty and Susan S. Golden
12. Novel strategies for the identification of clock genes in Neurospora with insertional mutagenesis
Kruno Sveric, Moyra Mason, Till Roenneberg and Martha Merrow
13. Mutagenesis with Drosophila
Patrick Emery
14. Mutagenesis in Arabidopsis.
Jodi Maple and Simon G. Møller
15. Yeast-two-hybrid screening.
Jodi Maple and Simon G. Møller
16. Microarrays: quality control and hybridization protocol.
Ken-ichiro Uno and Hiroki R. Ueda
17. Microarrays:statistical methods for circadian rhythms.
Rikuhiro Yamada and Hiroki R. Ueda
18. Identification of clock genes using difference gel electrophoresis (DIGE).
Natasha A. Karp and Kathryn S. Lilley
IV. Gene Expression: RNA.
19. Isolation of total RNA from Neurospora mycelium.
Cas Kramer
20. RNA extraction from Drosophila heads.
Patrick Emery
21. Extraction of plant RNA.
Michael G. Salter and Helen E. Conlon
22. RNA Extraction from mammalian tissues.
Stuart N. Peirson and Jason N. Butler
23. Northern analysis of sense and antisense frequency RNA in Neurospora crassa.
Cas Kramer
24. RNase protection assay.
Patrick Emery
25. Quantitative PCR.
Stuart N. Peirson and Jason N. Butler
V. Gene Expression: Proteins.
26. Protein extraction, fractionation and purification from cyanobacteria.
Natalia B. Ivleva and Susan S. Golden
27. Protein extraction from Drosophila heads.
Patrick Emery
28. Plant protein extraction.
Helen E. Conlon and Michael G. Salter
29. Protein extraction from mammalian tissues.
Choogon Lee
30. Western blotting.
Choogon Lee
31. Co-immunoprecipitation (coIP) assay.
Choogon Lee
32. In vitro phosphorylation and kinase assays in Neurospora crassa.
Lisa Franchi, Giuseppe Macino
VI. In vitro Systems.
33. Basic protocols for Drosophila S2 cell line: maintenance and transfection
M. Fernanda Ceriani
34. Co-immunoprecipitation on Drosophila cells in culture
M. Fernanda Ceriani
35. Basic protocols for zebrafish cell lines: maintenance and transfection.
Daniela Vallone, Cristina Santoriello, Srinivas Babu Gondi and Nicholas S. Foulkes
36. Manipulation of mammalian cell lines for circadian studies.
Filippo Tamani
Contributors
I. Overviews.
1. Light, photoreceptors and circadian clocks
Russell G. Foster, Mark W. Hankins and Stuart N. Peirson
2. Statistical analysis of biological rhythm data
Harold B. Dowse
II. Rhythmic Readouts.
3. Rhythmic conidiation in Neurospora crassa.
Cas Kramer
4. Monitoring and analyzing Drosophila circadian locomotor activity.
Mauro A. Zordan, Clara Benna and Gabriella Mazzotta.
5. Automated video image analysis of larval zebrafish locomotor rhythms.
Gregory M. Cahill
6. Locomotor activity in rodents.
Gianluca Tosini
7. Analysis of circadian leaf movement rhythms in Arabidopsis thaliana.
Kieron D. Edwards and Andrew J. Millar
8. Detection of rhythmic bioluminescence from luciferase reporters in cyanobacteria.
Shannon R. Canales, Jayna L. Ditty, Eugenia M. Clerico and Susan S. Golden
9. Analysis of rhythmic gene expression in adult Drosophila using the firefly-luciferase reporter gene.
Ralf Stanewsky
10. Monitoring circadian rhythms in Arabidopsis thaliana using luciferase reporter genes.
Anthony Hall and Paul Brown
III. Identification of Clock Genes.
11. Specialized techniques for site directed mutagenesis in cyanobacteria
Eugenia M. Clerico, Jayna L. Ditty and Susan S. Golden
12. Novel strategies for the identification of clock genes in Neurospora with insertional mutagenesis
Kruno Sveric, Moyra Mason, Till Roenneberg and Martha Merrow
13. Mutagenesis with Drosophila
Patrick Emery
14. Mutagenesis in Arabidopsis.
Jodi Maple and Simon G. Møller
15. Yeast-two-hybrid screening.
Jodi Maple and Simon G. Møller
16. Microarrays: quality control and hybridization protocol.
Ken-ichiro Uno and Hiroki R. Ueda
17. Microarrays:statistical methods for circadian rhythms.
Rikuhiro Yamada and Hiroki R. Ueda
18. Identification of clock genes using difference gel electrophoresis (DIGE).
Natasha A. Karp and Kathryn S. Lilley
IV. Gene Expression: RNA.
19. Isolation of total RNA from Neurospora mycelium.
Cas Kramer
20. RNA extraction from Drosophila heads.
Patrick Emery
21. Extraction of plant RNA.
Michael G. Salter and Helen E. Conlon
22. RNA Extraction from mammalian tissues.
Stuart N. Peirson and Jason N. Butler
23. Northern analysis of sense and antisense frequency RNA in Neurospora crassa.
Cas Kramer
24. RNase protection assay.
Patrick Emery
25. Quantitative PCR.
Stuart N. Peirson and Jason N. Butler
V. Gene Expression: Proteins.
26. Protein extraction, fractionation and purification from cyanobacteria.
Natalia B. Ivleva and Susan S. Golden
27. Protein extraction from Drosophila heads.
Patrick Emery
28. Plant protein extraction.
Helen E. Conlon and Michael G. Salter
29. Protein extraction from mammalian tissues.
Choogon Lee
30. Western blotting.
Choogon Lee
31. Co-immunoprecipitation (coIP) assay.
Choogon Lee
32. In vitro phosphorylation and kinase assays in Neurospora crassa.
Lisa Franchi, Giuseppe Macino
VI. In vitro Systems.
33. Basic protocols for Drosophila S2 cell line: maintenance and transfection
M. Fernanda Ceriani
34. Co-immunoprecipitation on Drosophila cells in culture
M. Fernanda Ceriani
35. Basic protocols for zebrafish cell lines: maintenance and transfection.
Daniela Vallone, Cristina Santoriello, Srinivas Babu Gondi and Nicholas S. Foulkes
36. Manipulation of mammalian cell lines for circadian studies.
Filippo Tamani