William C. Heiser
Humana Press
0e druk, 2010
9781617373497
Gene Delivery to Mammalian Cells
Volume 1: Nonviral Gene Transfer Techniques
Specificaties
Paperback, 301 blz.
|
Engels
Humana Press |
0e druk, 2010
ISBN13: 9781617373497
Rubricering
Onderdeel van serie
Methods in Molecular Biology
Verwachte levertijd ongeveer 9 werkdagen
Samenvatting
Highly experienced researchers describe in step-by-step detail methods that have proven most useful in delivering genes to mammalian cells. Volume 1: Nonviral Gene Transfer Techniques focuses on gene delivery by a variety of chemical and physical methods, including ultrasound, biolistics, peptides, PNA clamps, liposomes, microinjection, electroporation, particle bombardment, dendrimers, and hydrodynamics. An accompanying volume, Volume 2: Viral Gene Transfer Techniques, details procedures for delivering genes to cells in vitro and in vivo, including the use of lentiviral vectors.
Specificaties
ISBN13:9781617373497
Taal:Engels
Bindwijze:paperback
Aantal pagina's:301
Uitgever:Humana Press
Druk:0
Inhoudsopgave
Delivery Using Chemical Methods.- Chemical Methods for DNA Delivery.- Gene Transfer into Mammalian Cells Using Calcium Phosphate and DEAE-Dextran.- DNA Delivery to Cells in Culture Using Peptides.- DNA Delivery to Cells in Culture Using PNA Clamps.- Dendrimer-Mediated Cell Transfection in Vitro.- DNA Delivery to Cells in Culture Using Cationic Liposomes.- Formulation of Synthetic Gene Delivery Vectors for Transduction of the Airway Epithelium.- Cationic Liposome-Mediated DNA Delivery to the Lung Endothelium.- Delivery of DNA to Tumor Cells Using Cationic Liposomes.- Delivery of Transposon DNA to Lungs of Mice Using Polyethyleneimine-DNA Complexes.- Delivery Using Physical Methods.- Gene Delivery Using Physical Methods.- Gene Delivery to Mammalian Cells by Microinjection.- Delivery of DNA to Cells in Culture Using Particle Bombardment.- Delivery of DNA to Skin by Particle Bombardment.- Biolistic Transfection of Cultured Organotypic Brain Slices.- Efficient Electroporation of Mammalian Cells in Culture.- Delivery of DNA to Skin by Electroporation.- In Vivo DNA Electransfer in Skeletal Muscle.- Electrically Mediated Plasmid DNA Delivery to Solid Tumors In Vivo.- Hydrodynamic Delivery of DNA.- Naked DNA Gene Transfer in Mammalian Cells.- Microparticle Delivery of Plasmid DNA to Mammalian Cells.- DNA Delivery to Cells in Culture Using Ultrasound.- DNA Delivery to Cells In Vivo by Ultrasound.