,

Molecular Diagnosis of Infectious Diseases

Specificaties
Gebonden, 464 blz. | Engels
Humana Press | 2e druk, 2003
ISBN13: 9781588292216
Rubricering
Humana Press 2e druk, 2003 9781588292216
Onderdeel van serie Methods in Molecular Medicine
Verwachte levertijd ongeveer 9 werkdagen

Samenvatting

Populations of the western world are now healthier and enjoying higher life expectancy than ever. They are beginning to benefit from an array of costly new therapies made possible through recent rapid advances in medical science and technology, and their demands on modern medicine are rising. Meanwhile, healthcare systems are struggling with their outdated legacy models of the m- th 20 century and are experiencing ever-increasing financial pressure from g- ernments and health insurance organizations. The equation is no longer in balance, and this predicament is forcing societies to explore new approaches to managing healthcare in the future. Since the first edition of Molecular Diagnosis of Infectious Diseases was published, we have witnessed the sequencing of the (almost) complete human genome and a shift in medical research from an emphasis on genetics to the advancement and useful application of proteomics. Bioinformatics has become the key tool for managing and analyzing the upsurge of data, and faster and more effective test methods and technologies have opened up new prospects for ind- try and academia. The tools of modern genomics and proteomics are now being utilized to specifically guide the discovery of drugs for the prevention, diagnosis, and treatment of human disease. They may also help us to find a way out of the current healthcare calamity.

Specificaties

ISBN13:9781588292216
Taal:Engels
Bindwijze:gebonden
Aantal pagina's:464
Uitgever:Humana Press
Druk:2

Inhoudsopgave

Protein-Based Identification Of Antigens.- Proteomic Approaches to Antigen Discovery.- Immunoproteomics.- Immunoprecipitation and Blotting.- DNA-Based Identification of Antigens.- Representational Difference Analysis of cDNA.- Microarray Data Analysis and Mining.- Expression Cloning.- Identification of Epitopes and Immunomodulatory Components.- Determination of Epitopes by Mass Spectrometry.- Identification of T-Cell Epitopes Using ELISpot and Peptide Pool Arrays.- Virus-like Particles.- Application of Single-Cell Cultures of Mouse Splenocytes as an Assay System to Analyze the Immunomodulatory Properties of Bacterial Components.- Expression of Recombinant Proteins.- High-Throughput Expression and Purification of 6xHis-Tagged Proteins in a 96-Well Format.- Production of Antigens in Chlamydomonas reinhardtii.- Codon-Optimized Genes that Enable Increased Heterologous Expression in Mammalian Cells and Elicit Efficient Immune Responses in Mice after Vaccination of Naked DNA.- Purification, Modification, and Renaturation of Recombinant Proteins.- Purification and Immunological Characterization of Recombinant Antigens Expressed in the Form of Insoluble Aggregates (Inclusion Bodies).- Purification of Recombinant Proteins with High Isoelectric Points.- Refolding of Inclusion Body Proteins.- Small-Molecule-Protein Conjugation Procedures.- Characterization of Recombinant Proteins.- Structural Characterization of Proteins and Peptides.- Determination of Kinetic Data Using Surface Plasmon Resonance Biosensors.- Affinity Measurements of Biological Molecules by a Quartz Crystal Microbalance (QCM) Biosensor.- Evaluation of Recombinant Proteins in Immunological Test Systems.- Solid Supports in Enzyme-Linked Immunosorbent Assay and Other Solid-Phase Immunoassays.- Design and Preparationof Recombinant Antigens as Diagnostic Reagents in Solid-Phase Immunosorbent Assays.- Basic Problems of Serological Laboratory Diagnosis.- Molecular Diagnostics Resources on the Internet.- Recombinant Receptor Molecules.- Cloning Single-Chain Antibody Fragments (scFv) from Hybridoma Cells.

Rubrieken

    Personen

      Trefwoorden

        Molecular Diagnosis of Infectious Diseases