Molecular Neurosurgery with Targeted Toxins
Samenvatting
Pioneers and leading researchers explain the theory and techniques of using targeted toxins experimentally. The highly successful use of the 192 IgG-saporin and ME20.4-saporin immunotoxins to lesion the cholinergic basal forebrain in order to model the behavior, anatomy, physiology, and pharmacology of Alzheimer's disease in animals is treated in detail to give a potential user the knowledge to comfortably use the techniques involved. The uses of important new lesioning agents such as anti-DBH-saporin immunotoxin to make remarkably selective lesions of catecholaminergic neurons, hypocretin-saporin that can produce narcoleptic animals, and other saporin conjugates, such as neuropeptide-saporin conjugates for pain research and cholera toxin B chain-saporin to produce a model of CNS demyelination are explained by experts in the field.
Specificaties
Inhoudsopgave
Ronald G. Wiley and Douglas A. Lappi
Ribosome-Inactivating Proteins
Fiorenzo Stirpe
Biochemical, Physiological, and Behavioral Characterizations of the Cholinergic Basal Forebrain Lesion Produced by 192 IgG-Saporin
Jerene J. Waite
Basal Forebrain Cholinergic Lesion by 192 IgG-Saporin: A Tool to Assess the Consequences of Cortical Cholinergic Dysfunction in Alzheimer´s Disease
Reinhard Schliebs
192 IgG-Saporin-Induced Partial Cortical Cholinergic Deafferentation as a Model for Determining the Interactions Between Brain Aging and Neurodevelopmental Defects in the Cortical Cholinergic Input System
Martin Sarter and John P. Bruno
Exploring the Role of Acetylcholine in Primate Cognition Using Me20.4 IgG-Saporin
Rosalind M. Ridley and Harry F. Baker
Cortical Cholinergic Deafferentation Induces Ab Deposition: Toward a Physiological Animal Model of Alzheimer's Disease
Thomas G. Beach, D. G. Walker, P. E. Potter, L. I. Sue, S. Scott, K. J. Layne, A. J. Newell, P. K. Rauschkolb, M. E. Poston, S. D. Webster, R. A. Durham, M. R. Emmerling, K. Sawada, W. G. Honer, A. Fisher, and A. E. Roher
Chemical Dissection of Brain Glucoregulatory Circuitry
Sue Ritter, Thu T. Dinh, Kishor Bugarith, and Dawna M. Salter
Cardiovascular Deficits After Lesions of C1 Adrenergic Neurons With a Saporin-Based Immunotoxin
Patrice G. Guyenet, Ruth L. Stornetta, and Ann M. Schreihofer
Saporin Conjugates and Pain
Ronald G. Wiley and Douglas A. Lappi
The Use of Saporin Conjugates to Dissect Neurons Responsible for Sleep and Wakefulness
Carlos Blanco-Centurion, Dmitry Gerashchenko, Eric Murillo-Rodriguez, Frank Desarnaud, and Priyattam J. Shiromani
Isolectin B4-Mediated Cytotoxic Targeting of Sensory Neurons
Lucy Vulchanova and Christopher N. Honda
B Fragment of Cholera ToxinConjugated to Saporin
Peter T. Ohara, Kanwarjit Kelley, and Luc Jasmin
Index