Vascular Effects of Hydrogen Sulfide

Methods and Protocols

Specificaties
Paperback, blz. | Engels
Springer New York | e druk, 2020
ISBN13: 9781493995301
Rubricering
Springer New York e druk, 2020 9781493995301
Onderdeel van serie Methods in Molecular Biology
€ 61,99
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Samenvatting

This book explores techniques for exploring hydrogen sulfide (H2S) and its effects on the vascular system through numerous experimental animal models and vascular preparations. Alterations of vascular H2S generation/signaling may be involved in the pathogenesis of systemic and pulmonary arterial hypertension, ischemic heart disease, ischemic stroke, preeclampsia, and erectile dysfunction, and H2S also serves as an attractive target for pharmacotherapy of cardiovascular diseases, as well as possible effects on cancer, wound healing, and diabetic retinopathy, among other pathologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Vascular Effects of Hydrogen Sulfide: Methods and Protocols is an ideal aid for scientists working to extend our knowledge in this valuable and wide-ranging field of study.

Specificaties

ISBN13:9781493995301
Taal:Engels
Bindwijze:paperback
Uitgever:Springer New York

Inhoudsopgave

<p>Synthesis, Metabolism, and Signaling Mechanisms of Hydrogen Sulfide: An Overview.- Lanthionine&nbsp;and Other Relevant Sulfur Amino Acid Metabolites: Detection of&nbsp;Prospective Uremic Toxins&nbsp;in Serum&nbsp;by Multiple Reaction Monitoring Tandem Mass Spectrometry.- Analysis of Vascular Hydrogen Sulfide Biosynthesis.- Measurement of Protein Persulfidation: Improved Tag-Switch Method.- ProPerDP: A Protein Persulfide Detection Protocol.- Vascular Effects of H<sub>2</sub>S-Donors: Fluorimetric Detection of H<sub>2</sub>S Generation and Ion Channel Activation in Human Aortic Smooth Muscle Cells.- In Vitro Measurement of H<sub>2</sub>S-Mediated Vasoactive Responses.- In Vivo Measurement of H<sub>2</sub>S, Polysulfides, and ‘SSNO-mix’-Mediated Vasoactive Responses and Evaluation of 10 Hemodynamic Parameters from Rat Arterial Pulse Waveform.- Simultaneous Measurements of Tension and Free H<sub>2</sub>S in Mesenteric Arteries.- The Relaxant Mechanisms of Hydrogen Sulfide in Corpus Cavernosum.- Pharmacological Tools for the Study of H<sub>2</sub>S Contribution to Angiogenesis.- Central Administration of H<sub>2</sub>S Donors for Studying Cardiovascular Effects of H<sub>2</sub>S in Rats.- Colonic Delivery of H<sub>2</sub>S Donors for Studying Cardiovascular Effects of H<sub>2</sub>S in Rats.- Measurements for Sulfide-Mediated Inhibition of Myeloperoxidase Activity.- Vascular Myography to Examine Functional Responses of Isolated Blood Vessels.</p>
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        Vascular Effects of Hydrogen Sulfide