,

Brain Tumor Stem Cells

Methods and Protocols

Specificaties
Paperback, blz. | Engels
Springer New York | e druk, 2019
ISBN13: 9781493993949
Rubricering
Springer New York e druk, 2019 9781493993949
Onderdeel van serie Methods in Molecular Biology
€ 183,99
Levertijd ongeveer 8 werkdagen

Samenvatting

This detailed volume compiles the best methodologies and experimental techniques to profile and extract maximal data from brain tumor stem cells (BTSCs), the experimental paradigm for brain cancer research that offers insights into cancer stem cell populations that may drive not only tumor initiation but tumor recurrence and patient relapse. The BTSC model recapitulates scientific observations made in brain cancer patients, and these chapters provide the reader with a comprehensive understanding of the skills and techniques that will unlock data from this most informative subset of cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Brain Tumor Stem Cells: Methods and Protocolsserves as an ideal guide for researchers seeking to better understand the complexities of brain cancer.

Specificaties

ISBN13:9781493993949
Taal:Engels
Bindwijze:paperback
Uitgever:Springer New York

Inhoudsopgave

<p>Introduction to Brain Tumor Stem Cells.-&nbsp;Isolation and Culture of Glioblastoma Brain Tumor Stem Cells.-&nbsp;Establishment and Culture of Patient-Derived Primary Medulloblastoma Cell Lines.- Bioinformatic Strategies for the Genomic and Epigenomic Characterization of Brain Tumors.- Detecting Stem Cell Marker Expression Using the NanoString nCounter System.-&nbsp;Flow Cytometric Analysis of Brain Tumor Stem Cells.- In Vitro Self-Renewal Assays for Brain Tumor Stem Cells.- Differentiation of Brain Tumor Initiating Cells.-&nbsp;The Study of Brain Tumor Stem Cell Migration.- The Study of Brain Tumor Stem Cell Invasion.- Cell Cycle Dynamics in Glioma Cancer Stem Cells.- Embryonic Stem Cell Models of Human Brain Tumors.- Chromatin Immunoprecipitation (ChIP) Protocols for the Cancer and Developmental Biology Laboratory.- EPH Profiling of BTIC Populations in Glioblastoma Multiforme Using CyTOF.- Pooled Lentiviral CRISPR-Cas9 Screens for Functional Genomics in Mammalian Cells.-&nbsp;In Vitro Assays for Screening Small Molecules.- Drug Delivery in an Orthotopic Tumor Stem Cell-Based Model of Human Glioblastoma.- Engineering Inducible Knock-In Mice to Model Oncogenic Brain Tumor Mutations from Endogenous Loci.- In Vivo Murine Models of Brain Metastasis.- Cellular Magnetic Resonance Imaging for Tracking Metastatic Cancer Cells in the Brain.</p>
€ 183,99
Levertijd ongeveer 8 werkdagen

Rubrieken

    Personen

      Trefwoorden

        Brain Tumor Stem Cells