Bacterial Chromatin

Methods and Protocols

Specificaties
Paperback, blz. | Engels
Springer New York | e druk, 2019
ISBN13: 9781493993628
Rubricering
Springer New York e druk, 2019 9781493993628
Onderdeel van serie Methods in Molecular Biology
Verwachte levertijd ongeveer 8 werkdagen

Samenvatting

This volume brings together a wide range of methods to explore the structure and function of bacterial chromatin from molecular to the cellular scale. Chapters detail experimental protocols of in vivo and in vitro approaches, approaches to genome structure modeling, and data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Bacterial Chromatin: Methods and Protocols aims to be useful as an up-to-date reference work for scholars in the bacterial chromatin field, those entering the field from adjacent research fields, and scientists in the eukaryotic chromatin field.

Specificaties

ISBN13:9781493993628
Taal:Engels
Bindwijze:paperback
Uitgever:Springer New York

Inhoudsopgave

<p>1.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Determination of the 3D Genome Organization of Bacteria using Hi-C</p>

<p>Frédéric G. Crémazy, Fatema-Zahra M. Rashid, James R. Haycocks, Lisa E. Lamberte, David C. Grainger, and Remus T. Dame</p>

&nbsp;<p></p>

<p>2.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Overview of Processing and Analyzing Hi-C Data on Bacteria</p>

<p>Andreas Hofmann and Dieter W. Heermann </p>

&nbsp;<p></p>

<p>3.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; GeF-seq:A Simple Procedure for Base Pair Resolution ChIP-seq</p>

<p>Onuma Chumsakul,<sup> </sup>Kensuke Nakamura, Shu Ishikawa, and Taku Oshima</p>

<p>&nbsp;</p>

<p>4.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Genomic SELEX Screening of Regulatory Targets of Escherichia coli Transcription Factors</p>

<p>Tomohiro Shimada, Hiroshi Ogasawara, and Akira Ishihama</p>

<p>&nbsp;</p>

<p>5.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Modular assembly of Synthetic Secondary Chromosomes</p>

<p>Celine Zumkeller, Daniel Schindler, and Torsten Waldminghaus</p>

&nbsp;<p></p>

<p>6.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; High-resolution Characterization of DNA/Protein Complexes in Living Bacteria</p>

<p>Nicole A. Becker, Justin P. Peters, and L. James Maher, III</p>

<p>&nbsp;</p>

<p>7.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Imaging of Transcription and Replication in the Bacterial Chromosome with Multicolor Three-dimensional Superresolution Structured Illumination Microscopy </p>

<p>Carmen Mata Martin, Cedric Cagliero, Zhe Sun, and Ding Jun Jin </p>

<p>&nbsp;</p>

<p>8.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Genetic Approaches to Study the Interplay Between Transcription and Nucleoid-associated Proteins in Escherichia coli</p>

<p>Karin Schnetz</p>

<p>&nbsp;</p>

<p>&nbsp;</p>

<p>Part I: In vitro approaches</p>

<p>9.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Atomic Force Microscopy Imaging and Analysis of Prokaryotic Genome Organization</p>

<p>Ryosuke L. Ohniwa, Hugo Maruyama, Kazuya Morikawa, and Kunio Takeyasu</p>

<p>&nbsp;</p>

<p>10.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Dynamic Light Scattering of DNA-ligand Complexes</p>

Guangcan Yang and Yanwei Wang<p></p>

<p>&nbsp;</p>

<p>11.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Microscale Thermophoresis Analysis of Chromatin Interactions</p>

Ivan Corbeski, Velten Horn, Ramon A. van der Valk, Ulric le Paige, Remus T. Dame,<sup> </sup>and Hugo van Ingen<p></p>

<p>&nbsp;</p>

<p>12.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Quantitative Determination of DNA Bridging Efficiency of Chromatin Proteins</p>

<p>Ramon A. van der Valk, Qin Liang, Geri F. Moolenaar, <sup>&nbsp;</sup>and Remus T. Dame</p>

<p>&nbsp;</p>

<p>13.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Approaches for Determining DNA Persistence Length using Atomic Force Microscopy</p>

<p>Justin P. Peters and L. James Maher, III</p>

<p>&nbsp;</p>

<p>14.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Quantitation of DNA Binding Affinity using Tethered Particle Motion</p>

<p>Bram Henneman, Joost Heinsman, Julius Battjes, and Remus T. Dame</p>

<p>&nbsp;</p>

<p>15.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Observing Bacterial Chromatin Protein-DNA Interactions by Combining DNA Flow-stretching with Single-molecule Imaging</p>

<p>HyeongJun Kim and Joseph J. Loparo</p>

&nbsp;<p></p>

<p>16.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Unravelling the Biophysical Properties of Chromatin Proteins and DNA using Acoustic Force Spectroscopy<br> Szu-Ning Lin, Liang Qin, Gijs J. L. Wuite and Remus T. Dame</p>

<p>&nbsp;</p>

17.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Unravelling DNA organization with single-molecule Force Spectroscopy using Magnetic Tweezers<br> Thomas B. Brouwer, Artur Kaczmarczyk, Chi Pham, and John van Noort<p></p>

<p>&nbsp;</p>

<p>18.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; In Vitro Transcription Assay to Quantify Effects of H-NS Filaments on RNA Chain Elongation by RNA Polymerase<br> Beth A. Boudreau, Matthew V. Kotlajich, and Robert Landick</p>

<p>&nbsp;</p>

<p>&nbsp;</p>

<p>Part II: In Silico Approaches</p>

<p>&nbsp;</p>

<p>19.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Deciphering 3D Organization Of Chromosomes using Hi-C Data</p>

<p>Andreas Hofmann and Dieter W. Heermann </p>

<p>&nbsp;</p>

<p>20.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Molecular Dynamics Simulation of a Feather-boa Model of Bacterial Chromosome</p>

<p>Debasish Chaudhuri and Bela M. Mulder </p>

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