Luis A. Cañas,
José Pío Beltrán
Springer New York
e druk, 2019
9781493993482
Functional Genomics in Medicago truncatula
Methods and Protocols
Specificaties
Paperback, blz.
|
Engels
Springer New York |
e druk, 2019
ISBN13: 9781493993482
Rubricering
Onderdeel van serie
Methods in Molecular Biology
Levertijd ongeveer 8 werkdagen
Samenvatting
This volume discusses popular methods to achieve different types of mutagenesis and forward/reverse genetics in Medicago truncatula. Several studies on genetic control of developmental and metabolic processes in this model legume are also described. The chapters in this book cover topics such as Targeting Induced Local Lesions IN Genomes (TILLING), Fast Neutron Bombardment (FNB), Tnt1 insertional mutagenesis, Virus-Induced Gene Silencing (VIGS), stable inactivation of microRNAs in roots, gene editing by CRISPR-Cas9, etc. This book also contains reviews on the specific use of these techniques in functional studies on the genetic control of seed, leaf, root, nodule, floral and fruit development in M. truncatula. Written for the highly successful Methods in Molecular Biology series format, chapters contain the kind of detailed description and implementation advice needed to promote success in the lab.
Cutting-edge and thorough, Functional Genomics in Medicago truncatula: Methods and Protocols is a valuable resource for anyone interested in learning more about this developing field.
Specificaties
ISBN13:9781493993482
Taal:Engels
Bindwijze:paperback
Uitgever:Springer New York
Inhoudsopgave
<p>Chapter 1. Introduction </p>
<p>1.1. Legumes and agriculture sustainability</p>
<p>1.2. Grain and forage legumes: model systems used in developmental studies</p>
<p>1.3. Functional genomics in M. truncatula: applications in developmental studies</p>
<p>References</p>
<p> </p>
Chapter 2. M. truncatula genome sequencing: genomic data availability<p></p>
<p>Abstract</p>
<p>1. Introduction</p>
<p>2. The M. truncatula genome</p>
<p>3. Genomic data availability in M. truncatula</p>
<p>References</p>
<p></p>
<p>Chapter 3. Chemical mutagenesis in M. truncatula: ethyl methanesulphonate (EMS) and ethyl-nitrosourea (ENU)</p>
<p>Abstract</p>
<p>1. Introduction</p>
<p>2. Materials</p>
<p>3. Methods</p>
<p>4. Notes</p>
References<p></p>
<p> </p>
<p>Chapter 4. Physical mutagenesis in M. truncatula: g-rays and Fast Neutron Bombardment (FNB)</p>
<p>Abstract</p>
<p>1. Introduction</p>
<p>2. Materials</p>
<p>3. Methods</p>
<p>4. Notes</p>
<p>References</p>
<p> </p>
<p>Chapter 5. T-DNA insertional mutagenesis and activation tagging in M. truncatula</p>
<p>Abstract</p>
<p>1. Introduction</p>
<p>2. Materials</p>
<p>3. Methods</p>
<p>4. Notes</p>
<p>References</p>
<p> </p>
<p>Chapter 6. Tnt1 insertional mutagenesis in M. truncatula</p>
<p>Abstract</p>
<p>1. Introduction</p>
2. Materials<p></p>
<p>3. Methods</p>
<p>4. Notes</p>
<p>References</p>
<p> </p>
<p>Chapter 7. Target Induced Local Lesions IN Genomes (TILLING) in M. truncatula</p>
<p>Abstract</p>
1. Introduction<p></p>
<p>2. Materials</p>
<p>3. Methods</p>
<p>4. Notes</p>
<p>References</p>
<p> </p>
<p>Chapter 8. Transient post-transcriptional gene silencing in M. truncatula: Virus-Induced Gene Silencing (VIGS) </p>
Abstract<p></p>
<p>1. Introduction</p>
<p>2. Materials</p>
<p>3. Methods</p>
<p>4. Notes</p>
<p>References</p>
<p> </p>
<p>Chapter 9. Stable post-transcriptional gene silencing in M. truncatula: RNAi </p>
<p>Abstract</p>
<p>1. Introduction</p>
<p>2. Materials</p>
<p>3. Methods</p>
<p>4. Notes</p>
<p>References</p>
<p> </p>
Chapter 10. Gene silencing quantification in M. truncatula: PCR-based methods (semi- and quantitative RT-PCR)<p></p>
<p>Abstract</p>
<p>1. Introduction</p>
<p>2. Materials</p>
<p>3. Methods</p>
<p>4. Notes</p>
References<p></p>
<p> </p>
<p>Chapter 11. Gene silencing quantification in M. truncatula: fluorescence in situ<br> hibridization (FISH)</p>
<p>1. Introduction</p>
<p>2. Materials</p>
<p>3. Methods</p>
<p>4. Notes</p>
<p>References</p>
<p><br> Chapter 12. Functional genomics to study seed development in M. truncatula: an overview</p>
<p>Abstract</p>
<p>1. Introduction</p>
<p>2. Functional genomics and genetic control of seed development in M. truncatula</p>
<p>References</p>
<p></p>
<p>Chapter 13. Functional genomics to study plant architecture and branching in M. truncatula: an overview</p>
<p>Abstract</p>
<p>1. Introduction</p>
<p>2. Functional genomics and genetic control of plant architecture and branching in M. truncatula</p>
<p>References</p>
<p> </p>
<p>Chapter 14. Functional genomics to study compound leaf development in M. truncatula: an overview</p>
<p>Abstract</p>
<p>1. Introduction</p>
<p>2. Functional genomics and genetic control of compound leaf development in M. truncatula</p>
<p>References</p>
<p> </p>
Chapter 15. Functional genomics and M. truncatula root and nodule development: an overview<p></p>
<p>Abstract</p>
<p>1. Introduction</p>
<p>2. Functional genomics and genetic control of root and nodule development in M. truncatula</p>
<p>References</p>
<p> </p>
<p>Chapter 16. Functional genomics and M. truncatula flowering time: an overview</p>
<p>Abstract</p>
<p>1. Introduction</p>
<p>2. Functional genomics and genetic control of floral development in M. truncatula</p>
<p>References</p>
<p> </p>
<p>Chapter 17. Functional genomics and M. truncatula floral development: an overview</p>
<p>Abstract</p>
<p>1. Introduction</p>
<p>2. Functional genomics and genetic control of floral development in M. truncatula</p>
<p>References</p>
<p> </p>
<p>Chapter 18. Functional genomics and M. truncatula fruit development: an overview</p>
Abstract<p></p>
<p>1. Introduction</p>
<p>2. Functional genomics and genetic control of fruit development in M. truncatula</p>
<p>References</p>
<p> </p>
<p>Chapter 19. Emerging tools in functional genomics of M. truncatula: the CRISPR-Cas9 system</p>
<p>Abstract</p>
<p>1. Introduction</p>
<p>2. Editing the M. truncatula genome: targeted mutagenesis and gene editing using the CRISPR-Cas9 system</p>
<p>References</p>
<p> </p>
<p>Chapter 20. Future prospects in functional genomics of legumes</p>
1. Introduction<p></p>
<p>2. New legumes created through genome engineering versus transgenic ones carrying foreign DNA in their genomes: will be more acceptable for the consumers?</p>
<p>References</p>
<p>1.1. Legumes and agriculture sustainability</p>
<p>1.2. Grain and forage legumes: model systems used in developmental studies</p>
<p>1.3. Functional genomics in M. truncatula: applications in developmental studies</p>
<p>References</p>
<p> </p>
Chapter 2. M. truncatula genome sequencing: genomic data availability<p></p>
<p>Abstract</p>
<p>1. Introduction</p>
<p>2. The M. truncatula genome</p>
<p>3. Genomic data availability in M. truncatula</p>
<p>References</p>
<p></p>
<p>Chapter 3. Chemical mutagenesis in M. truncatula: ethyl methanesulphonate (EMS) and ethyl-nitrosourea (ENU)</p>
<p>Abstract</p>
<p>1. Introduction</p>
<p>2. Materials</p>
<p>3. Methods</p>
<p>4. Notes</p>
References<p></p>
<p> </p>
<p>Chapter 4. Physical mutagenesis in M. truncatula: g-rays and Fast Neutron Bombardment (FNB)</p>
<p>Abstract</p>
<p>1. Introduction</p>
<p>2. Materials</p>
<p>3. Methods</p>
<p>4. Notes</p>
<p>References</p>
<p> </p>
<p>Chapter 5. T-DNA insertional mutagenesis and activation tagging in M. truncatula</p>
<p>Abstract</p>
<p>1. Introduction</p>
<p>2. Materials</p>
<p>3. Methods</p>
<p>4. Notes</p>
<p>References</p>
<p> </p>
<p>Chapter 6. Tnt1 insertional mutagenesis in M. truncatula</p>
<p>Abstract</p>
<p>1. Introduction</p>
2. Materials<p></p>
<p>3. Methods</p>
<p>4. Notes</p>
<p>References</p>
<p> </p>
<p>Chapter 7. Target Induced Local Lesions IN Genomes (TILLING) in M. truncatula</p>
<p>Abstract</p>
1. Introduction<p></p>
<p>2. Materials</p>
<p>3. Methods</p>
<p>4. Notes</p>
<p>References</p>
<p> </p>
<p>Chapter 8. Transient post-transcriptional gene silencing in M. truncatula: Virus-Induced Gene Silencing (VIGS) </p>
Abstract<p></p>
<p>1. Introduction</p>
<p>2. Materials</p>
<p>3. Methods</p>
<p>4. Notes</p>
<p>References</p>
<p> </p>
<p>Chapter 9. Stable post-transcriptional gene silencing in M. truncatula: RNAi </p>
<p>Abstract</p>
<p>1. Introduction</p>
<p>2. Materials</p>
<p>3. Methods</p>
<p>4. Notes</p>
<p>References</p>
<p> </p>
Chapter 10. Gene silencing quantification in M. truncatula: PCR-based methods (semi- and quantitative RT-PCR)<p></p>
<p>Abstract</p>
<p>1. Introduction</p>
<p>2. Materials</p>
<p>3. Methods</p>
<p>4. Notes</p>
References<p></p>
<p> </p>
<p>Chapter 11. Gene silencing quantification in M. truncatula: fluorescence in situ<br> hibridization (FISH)</p>
<p>1. Introduction</p>
<p>2. Materials</p>
<p>3. Methods</p>
<p>4. Notes</p>
<p>References</p>
<p><br> Chapter 12. Functional genomics to study seed development in M. truncatula: an overview</p>
<p>Abstract</p>
<p>1. Introduction</p>
<p>2. Functional genomics and genetic control of seed development in M. truncatula</p>
<p>References</p>
<p></p>
<p>Chapter 13. Functional genomics to study plant architecture and branching in M. truncatula: an overview</p>
<p>Abstract</p>
<p>1. Introduction</p>
<p>2. Functional genomics and genetic control of plant architecture and branching in M. truncatula</p>
<p>References</p>
<p> </p>
<p>Chapter 14. Functional genomics to study compound leaf development in M. truncatula: an overview</p>
<p>Abstract</p>
<p>1. Introduction</p>
<p>2. Functional genomics and genetic control of compound leaf development in M. truncatula</p>
<p>References</p>
<p> </p>
Chapter 15. Functional genomics and M. truncatula root and nodule development: an overview<p></p>
<p>Abstract</p>
<p>1. Introduction</p>
<p>2. Functional genomics and genetic control of root and nodule development in M. truncatula</p>
<p>References</p>
<p> </p>
<p>Chapter 16. Functional genomics and M. truncatula flowering time: an overview</p>
<p>Abstract</p>
<p>1. Introduction</p>
<p>2. Functional genomics and genetic control of floral development in M. truncatula</p>
<p>References</p>
<p> </p>
<p>Chapter 17. Functional genomics and M. truncatula floral development: an overview</p>
<p>Abstract</p>
<p>1. Introduction</p>
<p>2. Functional genomics and genetic control of floral development in M. truncatula</p>
<p>References</p>
<p> </p>
<p>Chapter 18. Functional genomics and M. truncatula fruit development: an overview</p>
Abstract<p></p>
<p>1. Introduction</p>
<p>2. Functional genomics and genetic control of fruit development in M. truncatula</p>
<p>References</p>
<p> </p>
<p>Chapter 19. Emerging tools in functional genomics of M. truncatula: the CRISPR-Cas9 system</p>
<p>Abstract</p>
<p>1. Introduction</p>
<p>2. Editing the M. truncatula genome: targeted mutagenesis and gene editing using the CRISPR-Cas9 system</p>
<p>References</p>
<p> </p>
<p>Chapter 20. Future prospects in functional genomics of legumes</p>
1. Introduction<p></p>
<p>2. New legumes created through genome engineering versus transgenic ones carrying foreign DNA in their genomes: will be more acceptable for the consumers?</p>
<p>References</p>