Nathaniel Shank
Springer New York
e druk, 2020
9781493992188
Non-Natural Nucleic Acids
Methods and Protocols
Specificaties
Paperback, blz.
|
Engels
Springer New York |
e druk, 2020
ISBN13: 9781493992188
Rubricering
Onderdeel van serie
Methods in Molecular Biology
Levertijd ongeveer 8 werkdagen
Samenvatting
This volume provides relevant synthetic strategies, incorporation, and applications of non-natural nucleic acids. Chapters detail monomer synthesis, oligomer synthesis/construction, and applications allowing researchers to explore and determine which methodology or methodologies are relevant to their needs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Non-Natural Nucleic Acids: Methods and Protocols aims to serve as a guide for researchers exploring their own inquiries and to provide a springboard for new endeavors.
Specificaties
ISBN13:9781493992188
Taal:Engels
Bindwijze:paperback
Uitgever:Springer New York
Inhoudsopgave
<p>1. Synthesis and Enzymatic Characterization of Sugar-Modified Nucleoside Triphosphate Analogs</p>
<p> Stella Diafa, Damien Evéquoz, Christian J. Leumann, and Marcel Hollenstein</p>
<p> </p>
<p>2. Synthesis of Site-Specific Crown Ether Adducts to DNA Abasic Sites, 8-Oxo-7,8-Dihydro-2’-Deoxyguanosine and 2’-Deoxycytidine</p>
<p> Na An, Aaron M. Fleming, Nicole C. Rosecrans, Yi Liao, and Cynthia J. Burrows</p>
<p> </p>
<p>3. Synthesis of a Fluorescent Cytidine TNA Triphosphate Analogue</p>
<p> Hui Mei and John Chaput</p>
<p> </p>
<p>4. Synthesis of Base-Modified dNTPs through Cross-Coupling Reactions and Their Polymerase Incorporation to DNA</p>
<p> Petra Ménová, Hana Cahová, Milan Vrábel, and Michal Hocek</p>
<p> </p>
<p>5. 2ʹ-C,4ʹ-C-Ethyleneoxy-Bridged 2ʹ-Deoxyribonucleic Acids (EoDNAs) with Thymine Nucleobases: Synthesis, Duplex-Forming Ability, and Enzymatic Stability</p>
<p> Takashi Osawa, Satoshi Obika, and Yoshiyuki Hari</p>
<p> </p>
<p>6. Synthesis Protocols for Simple Uncharged Glycol Carbamate Nucleic Acids</p>
<p> Tanaya Bose and Vaijayanti A. Kumar</p>
<p> </p>
<p>7. Synthesis of Nucleobase-Functionalized Morpholino Monomers</p>
<p> Bappaditya Nandi, Sankha Pattanayak, Sibasish Paul, Jayanta Kundu, and Surajit Sinha</p>
<p> </p>
<p>8. Synthesis and Application of <sup>L</sup>KγT Peptide Nucleic Acids</p>
<p> Nathaniel Shank, Kara M. George Rosenker, Ethan A. Englund, Andrew V. Dix, Elizabeth E. Rastede, and Daniel H. Appella</p>
<p> </p>
<p>9. Aminoglycoside Functionalization as a Tool for Targeting Nucleic Acids</p>
<p> Derrick Watkins, Krishnagopal Maiti, and Dev P. Arya</p>
<p></p>
<p>10. Preparation and Purification of Oligodeoxynucleotide Duplexes Containing a Site-Specific, Reduced, Chemically-Stable Covalent Interstrand Cross-Link between a Guanine Residue and an Abasic Site</p>
<p> Maryam Imani Nejad, Xu Guo, Kurt Housh, Christopher Nel, Zhiyu Yang, Nathan E. Price, Yinsheng Wang, and Kent S. Gates</p>
<p> </p>
<p>11. Copper-Catalyzed Alkyne-Azide Cycloaddition on the Solid Phase for the Preparation of Fully Click-Modified Nucleic Acids</p>
<p> Malte Rosenthal, Franziska Pfeiffer, and Günter Mayer</p>
<p> </p>
<p>12. Labeling Peptide Nucleic Acids with Indium-111</p>
<p> Igor G. Panyutin</p>
<p> </p>
<p>13. Site-Specific Labeling of DNA via PCR with an Expanded Genetic Alphabet</p>
<p> Michael P. Ledbetter, Denis A. Malyshev, and Floyd E. Romesberg</p>
<p> </p>
<p>14. Flexible Nucleic Acids (FNAs) As Informational Molecules: Enzymatic Polymerization of fNTPs on DNA Templates and Nonenzymatic Oligomerization of RNA on FNA Templates</p>
<p> Maryline Chemama and Christopher Switzer</p>
<p> </p>
<p>15. Artificial Nucleosides as Diagnostic Probes to Measure Translesion DNA Synthesis</p>
<p> Jung-Suk Choi and Anthony Berdis</p>
<p></p>
<p>16. FRET Assay for Ligands Targeting the Bacterial A-Site RNA</p>
<p> Renatus W. Sinkeldam and Yitzhak Tor</p>
<p> </p>
<p>17. The Use of Serinol Nucleic Acids as Ultrasensitive Molecular Beacons</p>
<p> Keiji Murayama, Hiromu Kashida, and Hiroyuki Asanuma</p>
<p> </p>
<p>18. Oligonucleotide Primers with G<sup>8AE</sup>-Clamp Modifications for RT-qPCR Detection of the Low-Copy dsRNA</p>
<p> Timofei S. Zatsepin, Anna M. Varizhuk, Vladimir G. Dedkov, German A. Shipulin, and Andrey V. Aralov</p>
<p> </p>
<p>19. Determining Steady-State Kinetics of DNA Polymerase Nucleotide Incorporation</p>
<p> Hailey L. Gahlon and Shana J. Sturla</p>
<p> Stella Diafa, Damien Evéquoz, Christian J. Leumann, and Marcel Hollenstein</p>
<p> </p>
<p>2. Synthesis of Site-Specific Crown Ether Adducts to DNA Abasic Sites, 8-Oxo-7,8-Dihydro-2’-Deoxyguanosine and 2’-Deoxycytidine</p>
<p> Na An, Aaron M. Fleming, Nicole C. Rosecrans, Yi Liao, and Cynthia J. Burrows</p>
<p> </p>
<p>3. Synthesis of a Fluorescent Cytidine TNA Triphosphate Analogue</p>
<p> Hui Mei and John Chaput</p>
<p> </p>
<p>4. Synthesis of Base-Modified dNTPs through Cross-Coupling Reactions and Their Polymerase Incorporation to DNA</p>
<p> Petra Ménová, Hana Cahová, Milan Vrábel, and Michal Hocek</p>
<p> </p>
<p>5. 2ʹ-C,4ʹ-C-Ethyleneoxy-Bridged 2ʹ-Deoxyribonucleic Acids (EoDNAs) with Thymine Nucleobases: Synthesis, Duplex-Forming Ability, and Enzymatic Stability</p>
<p> Takashi Osawa, Satoshi Obika, and Yoshiyuki Hari</p>
<p> </p>
<p>6. Synthesis Protocols for Simple Uncharged Glycol Carbamate Nucleic Acids</p>
<p> Tanaya Bose and Vaijayanti A. Kumar</p>
<p> </p>
<p>7. Synthesis of Nucleobase-Functionalized Morpholino Monomers</p>
<p> Bappaditya Nandi, Sankha Pattanayak, Sibasish Paul, Jayanta Kundu, and Surajit Sinha</p>
<p> </p>
<p>8. Synthesis and Application of <sup>L</sup>KγT Peptide Nucleic Acids</p>
<p> Nathaniel Shank, Kara M. George Rosenker, Ethan A. Englund, Andrew V. Dix, Elizabeth E. Rastede, and Daniel H. Appella</p>
<p> </p>
<p>9. Aminoglycoside Functionalization as a Tool for Targeting Nucleic Acids</p>
<p> Derrick Watkins, Krishnagopal Maiti, and Dev P. Arya</p>
<p></p>
<p>10. Preparation and Purification of Oligodeoxynucleotide Duplexes Containing a Site-Specific, Reduced, Chemically-Stable Covalent Interstrand Cross-Link between a Guanine Residue and an Abasic Site</p>
<p> Maryam Imani Nejad, Xu Guo, Kurt Housh, Christopher Nel, Zhiyu Yang, Nathan E. Price, Yinsheng Wang, and Kent S. Gates</p>
<p> </p>
<p>11. Copper-Catalyzed Alkyne-Azide Cycloaddition on the Solid Phase for the Preparation of Fully Click-Modified Nucleic Acids</p>
<p> Malte Rosenthal, Franziska Pfeiffer, and Günter Mayer</p>
<p> </p>
<p>12. Labeling Peptide Nucleic Acids with Indium-111</p>
<p> Igor G. Panyutin</p>
<p> </p>
<p>13. Site-Specific Labeling of DNA via PCR with an Expanded Genetic Alphabet</p>
<p> Michael P. Ledbetter, Denis A. Malyshev, and Floyd E. Romesberg</p>
<p> </p>
<p>14. Flexible Nucleic Acids (FNAs) As Informational Molecules: Enzymatic Polymerization of fNTPs on DNA Templates and Nonenzymatic Oligomerization of RNA on FNA Templates</p>
<p> Maryline Chemama and Christopher Switzer</p>
<p> </p>
<p>15. Artificial Nucleosides as Diagnostic Probes to Measure Translesion DNA Synthesis</p>
<p> Jung-Suk Choi and Anthony Berdis</p>
<p></p>
<p>16. FRET Assay for Ligands Targeting the Bacterial A-Site RNA</p>
<p> Renatus W. Sinkeldam and Yitzhak Tor</p>
<p> </p>
<p>17. The Use of Serinol Nucleic Acids as Ultrasensitive Molecular Beacons</p>
<p> Keiji Murayama, Hiromu Kashida, and Hiroyuki Asanuma</p>
<p> </p>
<p>18. Oligonucleotide Primers with G<sup>8AE</sup>-Clamp Modifications for RT-qPCR Detection of the Low-Copy dsRNA</p>
<p> Timofei S. Zatsepin, Anna M. Varizhuk, Vladimir G. Dedkov, German A. Shipulin, and Andrey V. Aralov</p>
<p> </p>
<p>19. Determining Steady-State Kinetics of DNA Polymerase Nucleotide Incorporation</p>
<p> Hailey L. Gahlon and Shana J. Sturla</p>