Plant Genome Editing with CRISPR Systems

<div>Preface…</div><div>Table of Contents…</div><div>Contributing Authors…</div><div><br></div><div>Part I Review on Plant DNA Repair and Genome Editing</div><div>1. Plant DNA Repair Pathways and their Applications in Genome Editing</div><div>Qiudeng Que, Zhongying Chen, Tim Kelliher, David Skibbe, Shujie Dong, and Mary-Dell Chilton&nbsp;</div><div><br></div><div>Part II CRISPR Design and Mutation Analysis</div><div><br></div><div>2. Rapid Screening of CRISPR/Cas9-Induced Mutants using the ACT-PCR Method</div><div>Chun Wang and Kejian Wang</div><div><br></div><div>3. Decoding Sanger-Sequencing Chromatograms from CRISPR-Induced Mutations</div><div>Xianrong Xie, Xingliang Ma, and Yao-Guang Liu</div><div><br></div><div>Part III Multiplexed CRISPR-Cas9 Systems&nbsp;</div><div><br></div><div>4. Creating Large Chromosomal Deletions in Rice using CRISPR/Cas9</div><div>Riqing Li, Si Nian Char, and Bing Yang</div><div><br></div><div>5. A Multiplexed CRISPR-Cas9 Editing System Based on the Endogenous tRNA Processing</div><div>Kabin Xie and Yinong Yang</div><div><br></div><div>6. A Single Transcript CRISPR-Cas9 System for Multiplex Genome Editing in Plants</div><div>Xu Tang, Zhaohui Zhong, Qiurong Ren, Binglin Liu, and Yong Zhang</div><div><br></div><div>7. CRISPR-Act2.0: An Improved Multiplexed System for Plant Transcriptional Activation</div><div>Aimee Malzahn, Yong Zhang, and Yiping Qi</div><div><br></div><div>Part IV CRISPR-Cas9 Editing in Monocots</div><div><br></div><div>8. Generating Photoperiod-Sensitive Genic Male Sterile Rice Lines with CRISPR-Cas9</div><div>Weihang Gu, Dabing Zhang, Yiping Qi, and Zheng Yuan</div><div><br></div><div>9. Knocking Out MicroRNA Genes in Rice with CRISPR/Cas9</div><div>Jianping Zhou, Zhaohui Zhong, Hongqiao Chen, Mengqiao Yang, Qian Li, Xuelian Zheng, Yiping Qi, and Yong Zhang</div><div><br></div><div>10. An Agrobacterium-Mediated CRISPR-Cas9 Platform for Genome Editing in Maize</div><div>Keunsub Lee, Huilan Zhu, Bing Yang, and Kan Wang</div><div><br></div><div>Part V CRISPR-Cas9 Editing in Dicots</div><div><br></div><div>11. Fluorescence Marker-Assisted Isolation of Cas9-Free and CRISPR-Edited Arabidopsis Plants</div><div>Hanchuanzhi Yu and Yunde Zhao</div><br><div>12. Creating Targeted Gene Knock-Outs in Brassica oleracea using CRISPR/Cas9</div><div>Tom Lawrenson, Penny Hundleby, and Wendy Harwood</div><div><br></div><div>13. Application of CRISPR/Cas9-Mediated Gene Editing in Tomato</div><div>Nathan T. Reem and Joyce Van Eck</div><div><br></div><div>14. Genome Editing in Potato with CRISPR/Cas9</div><div>Satya Swathi Nadakuduti, Colby G. Starker, Daniel F. Voytas, C. Robin Buell, and David S. Douches</div><div><br></div><div>15. Visual Assay for Gene Editing using a CRISPR/Cas9 System in Carrot Cells</div><div>Magdalena Klimek-Chodacka, Tomasz Oleszkiewicz, and Rafal Baranski</div><br><div>16. Genome Editing in Soybean with CRISPR/Cas9</div><div>Junqi Liu, Samatha Gunapati, Nicole T. Mihelich, Adrian O. Stec, Jean-Michel Michno, and Robert M. Stupar</div><div><br></div><div>17. Genome Editing in Citrus Tree with CRISPR/Cas9</div><div>Hongge Jia, Xiuping Zou, Vladimir Orbovic, and Nian Wang</div><br><div>Part VI CRISPR-Cas12a Editing Systems</div><div><br></div><div>18. Plant Gene Knockout and Knockdown by CRISPR-Cpf1 (Cas12a) Systems</div><div>Yingxiao Zhang, Yong Zhang, and Yiping Qi</div><div><br></div><div>19. Editing a Stomatal Developmental Gene in Rice with CRISPR/Cpf1</div><div>Abhishek Anand, Xiaojia Yin, Paul Quick, and Anindya Bandyopadhyay</div><div><br></div><div>20. Targeted Mutagenesis using FnCpf1 in Tobacco</div><div>Akira Endo and Seiichi Toki</div><div><br></div><div>Part VII Precise Gene Editing</div><div><br></div><div>21. Gene Replacement by Intron Targeting with CRISPR-Cas9</div><div>Jun Li, Xiangbing Meng, Jiayang Li, and Caixia Gao</div><div><br></div><div>22. Targeted Base Editing with CRISPR-Deaminase in Tomato</div><div>Zenpei Shimatani, Tohru Ariizumi, Ushio Fujikura, Akihiko Kondo, Hiroshi Ezura, and Keiji Nishida</div><div><br></div><div>Part VIII Non-Agrobacterium Based CRISPR Delivery Systems</div><div><br></div><div>23. Virus-Mediated Genome Editing in Plants using the CRISPR/Cas9 System</div><div>Ahmed Mahas, Zahir Ali, Manal Tashkandi, and Magdy M. Mahfouz</div><div><br></div><div>24. Biolistic Delivery of CRISPR/Cas9 with Ribonucleoprotein Complex in Wheat</div><div>Zhen Liang, Kunling Chen, and Caixia Gao</div><div><br></div><div>25. DNA-Free Genome Editing via Ribonucleoprotein (RNP) Delivery of CRISPR/Cas in Lettuce</div><div>Jongjin Park, Aiden Y. Park, Slki Park, Jiyoung Yoon, Sunmee Choi, and Sunghwa Choe</div><div><br></div><div>26. An Automated Protoplast Transformation System</div>Scott C. Lenaghan and C. Neal Stewart, Jr.