Gap Junction Protocols
Samenvatting
Presenting
state-of-the-art protocols to study gap junctions, this detailed book first
focuses on the use of methods and tools to investigate the different aspects of
connexin expression and gap junction regulation. The second part of the volume
describes several methods to probe gap junction functionality as such. Written
in the highly successful Methods in Molecular Biology series format, chapters
include introductions to their respective topics, lists of the necessary
materials and reagents, step-by-step and readily reproducible laboratory
protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative
and practical, Gap Junction Protocols is intended for basic and applied
researchers in the area of biomedical and life sciences, both in academic and
industrial settings.
Specificaties
Inhoudsopgave
Using Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction.- DNA Methylation Analysis of Human
Tissue-Specific Connexin Genes.- Detection of Connexins in Liver Cells Using
Sodiumdodecylsulfate Polyacrylamide Gel Electrophoresis and Immunoblot Analysis.- Immunohisto- and
Cytochemistry Analysis of Connexins.- Small Interfering RNA-Mediated
Connexin Gene Knockdown in Vascular Endothelial and Smooth Muscle Cells.- Generation and Use of Trophoblast
Stem Cells and Uterine Myocytes to Study the Role of Connexins for Pregnancy
and Labor.- Identification of Connexin43
Phosphorylation and S-Nitrosylation
in Cultured Primary Vascular Cells.- Preparation of Gap Junctions in
Membrane Microdomains for Immunoprecipitation and Mass Spectrometry Interactome
Analysis.- Scrape Loading/Dye Transfer Assay.- Microinjection Technique for Assessment of Gap
Junction Function.- Electroporation Loading and Dye
Transfer: A Safe and Robust Method to Probe Gap Junctional Coupling.- Using Fluorescence Recovery After Photobleaching to Study Gap Junctional
Communication In Vitro.- Tracking Dynamic Gap Junctional Coupling in Live Cells by Local
Photo-Activation and Fluorescence Imaging.- A Functional Assay to Assess Connexin43-Mediated Cell-to-Cell Communication of
Second Messengers in Cultured Bone Cells.- Calcium Wave Propagation Triggered by Local Mechanical Stimulation as a Method
for Studying Gap Junctions and Hemichannels.- Establishment of the Dual Whole Cell Recording Patch Clamp Configuration for
the Measurement of Gap Junction Conductance.</p>