Immobilization of Enzymes and Cells

Specificaties
Paperback, blz. | Engels
Humana Press | 3e druk, 2016
ISBN13: 9781493959884
Rubricering
Humana Press 3e druk, 2016 9781493959884
Onderdeel van serie Methods in Molecular Biology
€ 132,99
Levertijd ongeveer 8 werkdagen

Samenvatting

For most of industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols,  in order to be re-used for very long periods of time. From this point of view, immobilization, simplicity and stabilization have to be strongly related concepts. The third edition of Immobilization of Enzymes and Cells expands upon and updates the previous editions with current, detailed protocols for immobilization. With new chapters on protocols for immobilization of enzymes and cells which may be useful to greatly improve the functional properties of enzymes and cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Immobilization of Enzymes and Cells, Third Edition demonstrates simple and efficient protocols for the preparation, characterization, and utilization of immobilized enzymes and cells.

Specificaties

ISBN13:9781493959884
Taal:Engels
Bindwijze:paperback
Uitgever:Humana Press
Druk:3

Inhoudsopgave

<p>1. New Opportunities for Immobilization of Enzymes </p><p>Jose M. Guisan</p><p> </p>2. Immobilization of Enzymes: A Literature Survey Beatriz Brena, Paula González-Pombo, and Francisco Batista-Viera<p> </p><p>3. Glutaraldehyde Mediated Protein Immobilization</p><p>Fernando López-Gallego, Jose M. Guisán,and  Lorena Betancor<sup></sup></p><p> </p><p>4. Immobilization of Enzymes on Mono-and Hetero-Functional Epoxy-Activated Supports</p><p>Cesar Mateo, Valeria Grazu, and Jose M. Guisan<sup></sup></p><p> </p><p>5. Stabilization of Enzymes by Multipoint Covalent Immobilization on Supports Activated with Glyoxyl Groups</p><p>Fernando López-Gallego,  Gloria Fernandez-Lorente, Javier Rocha-Martin, Juan M. Bolivar, Cesar Mateo and  Jose M. Guisan</p><p> </p><p>6. Oriented Covalent Immobilization of Enzymes on Heterofunctional-Glyoxyl Supports</p><p>Cesar Mateo, Gloria Fernandez-Lorente, Rocha-Martin, J., Bolivar, Juan, M., Jose M. Guisan</p><p> </p><p>7. Reversible Covalent Immobilization of Enzymes Via Disulfide Bonds</p><p>Karen Ovsejevi , Carmen Manta, and  Francisco Batista-Viera</p><p> </p><p>8. Immobilization of Candida rugosa Lipase on Superparamagnetic Fe<sub>3</sub>O<sub>4</sub> Nanoparticles for Biocatalysis in Low Water Media</p><p>Joyeeta Mukherjee, Kusum Solanki, and Munishwar Nath Gupta<sup></sup></p><p> </p><p>9. Immobilization of Enzymes by Bioaffinity Layering</p><p>Veena Singh, Meryam Sardar, and  Munishwar Nath Gupta<sup></sup></p><p> </p><p>10. Immobilization of Enzymes on Magnetic Beads through Affinity Interactions</p><p>Audrey Sassolas, Akhtar Hayat and Jean-Louis Marty</p><p> </p><p>11. Tips for the Functionalization of  Nanoparticles with Antibodies</p><p>Ester Polo, Sara Puertas, María Moros, Pilar Batalla, José M. Guisán, Jesús M. de la Fuente, Valeria Grazú</p><p> </p><p>12. Design and Characterization of Functional Nanoparticles for Enhanced Bio-Performance</p><p>Pablo del Pino, Scott G. Mitchell &amp; Beatriz Pelaz</p><p> </p><p>13. Immobilization of Enzymes on Ethynyl-modified Electrodes Via Click Chemistry</p><p>Akhtar Hayat, Audrey Sassolas, Amina Rhouati, Jean-Louis Marty</p><p>14. Modification of Carbon Nanotube Electrodes with 1-pyrenebutanoic Acid, Succinimidyl Ester for Enhanced Bioelectrocatalysis </p><p>Guinevere Strack, Robert Nichols, Plamen Atanassov, Heather R. Luckarift, and Glenn R. Johnson</p><p> </p><p>15. Enzyme Immobilization by Entrapment within a Gel Network</p><p>Audrey Sassolas, Akhtar Hayat and Jean-Louis Marty</p><p> </p><p>16. Practical Protocols for Lipase Immobilization via Sol-Gel Techniques</p><p>Manfred T. Reetz</p><p> </p><p>17. Improving Lipase Activity by Immobilization and Post-Immobilization Strategies</p><p>Jose M. Palomo, Marco Filice, Oscar Romero and Jose M. Guisan</p><p> </p><p>18. High Activity Preparations of Lipases and Proteases for Catalysis in Low Water Containing Organic Solvents and Ionic Liquids</p><p>Ipsita Roy, Joyeeta Mukherjee<sub>, </sub><sup> </sup>and Munishwar Nath Gupta</p><p> </p><p>19. Biomedical Applications of Immobilized Enzymes: An Update</p><p>Marta Pastor, Amaia Esquisabel, and José Luis Pedraz </p> 20.  Immobilization of Whole Cells by Chemical Vapor Deposition of Silica <p>Susan R. Sizemore, Robert Nichols, Randi Tatum, Plamen Atanassov, Glenn R. Johnson, and Heather R. Luckarift</p><p> </p><p>21. Encapsulation of Cells in Alginate Gels</p><p>Pello Sánchez, Rosa María Hernández, José Luis Pedraz, and Gorka Orive </p><p> </p><p>22. Microalgal Immobilization Methods</p><p>Ignacio Moreno-Garrido</p><p> </p><p>23. Therapeutic Applications of Encapsulated Cells</p><p>Argia Acarregui, Gorka Orive, José Luis Pedraz, and Rosa María Hernández</p><p> </p><p>24. Whole Cell Entrapment Techniques </p><p>Jorge A. Trelles and Cintia W. Rivero</p><p> </p>
€ 132,99
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        Immobilization of Enzymes and Cells