<p>Part I. Microelectrode Techniques</p><p> </p><p>1. Patch-Clamp Protocols to Study Cell Ionic Homeostasis under Saline Conditions</p><p>Ana María Velarde-Buendía, René Alberto Enríquez-Figueroa, and Igor Pottosin</p><p> </p><p>2. Studying Plant Salt Tolerance with the Voltage Clamp Technique</p><p>Zhong-Hua Chen, Dezhi Wu, Cornelia Eisenach, Adrian Hills, Guoping Zhang, and Michael R. Blatt</p><p> </p><p>3. Using the Multifunctional Xylem Pprobe for In situ Studies of Plant Water and Ion Relations under Saline Conditions</p><p>Lars H. Wegner</p><p> </p><p>4. Measuring Intracellular Ion Concentrations with Multi-Barrelled Microelectrodes </p><p>Tony Miller and Susan Smith</p><p> </p><p>5. Single-Cell Sampling and Analysis (SiCSA)</p><p>Wieland Fricke</p><p> </p><p>6. Non-Invasive Flux Measurements Using Microsensors: Theory, Limitations and Systems</p><p>Ian Newman, Shao-Liang Chen, D. Marshall Porterfield, and Jian Sun</p><p> </p><p>7. Quantifying Kinetics of Net Ion Fluxes from Plant Tissues by Non-Invasive Microelectrode Measuring MIFE Technique</p><p>Sergey Shabala, Tracey Cuin, Lana Shabala and Ian Newman</p><p> </p><p>Part II. Imaging Techniques</p><p> </p><p>8. Quantitative Cryo-Analytical Scanning Electron Microscopy (CEDX): An Important Technique Useful for Cell-Specific Localization of Salt</p><p>Margaret McCully and Martin Canny</p><p> </p><p>9. Fluorescence Lifetime Imaging (FLIM) Measurements in Salinity Research</p><p>Olga Babourina and Zed Rengel</p><p> </p><p>10. Cytosolic Ca2+ Determinations in Studying Plant Responses to Salinity and Oxidative Stress</p><p>Anuphon Laohavisit, Renato Colaço, and Julia Davies</p><p> </p><p>11. Infrared Thermography in Plant Phenotyping for Salinity Tolerance</p><p>Richard A. James and Xavier R.R. Sirault</p><p> </p><p>12. In vivo Imaging of Nitric Oxide and Reactive Oxygen Species Using Laser Scanning Confocal Microscopy</p><p>Yan-Jie Xie and Wen-Biao Shen</p><p> </p><p>Part III. Biochemical Assays</p><p> </p><p>13. Metabolomics for Salinity Research</p><p>Ute Roessner and Diane M. Beckles</p><p> </p><p>14. Purification of Plant Plasma Membranes by Two-Phase Partitioning and Measurement of H+ Pumping</p><p>Anette Lund and Anja Thoe Fuglsang</p><p> </p><p>15. Determination of Reactive Oxygen Species in Salt-Stressed Plant Tissues</p><p>Andrés Alberto Rodríguez and Edith L. Taleisnik</p><p> </p><p>16. Quantification of the Antioxidant Activity in Salt-Stressed Tissues</p><p>Jelena J. Dragišić Maksimović and Branka D. Živanović</p><p> </p><p>17. Quantification of Abscisic Acid, Cytokinin and Auxin Content in Salt-Stressed Plant Tissues</p><p>Petre I. Dobrev and Radomira Vankova</p><p> </p><p> </p><p>Part IV. Molecular Techniques</p><p> </p><p>18. Fluorescence-Activated Cell Sorting for Analysis of Cell Type-Sspecific Responses to Salinity Stress in Arabidopsis and Rice</p><p>Aurelie Evrard, Bastiaan O. R. Bargmann, Kenneth D. Birnbaum, Mark Tester, Ute Baumann, and Alexander A. T. Johnson</p><p> </p><p>19. Transformation Using Controlled cDNA Overexpression System</p><p>Gábor Rigó, Csaba Papdi, and László Szabados</p><p> </p><p>20. Transcriptome Analysis of Membrane Transporters in Response to Salinity Stress</p><p>Prasad Senadheera1 and Frans J.M. Maathuis</p><p> </p><p>21. Marker-Assisted Selection in Plant Breeding for Salinity Tolerance</p><p>M. Ashraf, N. A. Akram, Mehboob-ur-Rahman, and M. R. Foolad</p><p> </p><p>22. Transcriptomics on Small Samples</p><p>Stuart J. Roy, Simon J. Conn, Gwenda M. Mayo, Asmini Athman, and Matthew Gilliham</p><p> </p><p>23. Plastid Transformation for Abiotic Stress Tolerance in Plants</p><p>K.C. Bansal, A. K. Singh, and S. H. Wani</p><p> </p><p>24. Manipulating Expression of Tonoplast Transporters</p><p>Zhigang Li, Man Zhou, Qian Hu, Shane Reighard, Shuangrong Yuan, Ning Yuan, Bekir San, Dayong Li, Haiyan Jia, and Hong Luo</p><p> </p><p>25. Using Heterologous Expression Systems to Characterize Potassium and Sodium Transport Activities</p><p>Alonso Rodríguez-Navarro1, Begoña Benito, and Olivier Cagnac</p><p> </p><p> </p><p>Part V. Other Methods</p><p> </p><p>26. Isotope Techniques to Study Kinetics of Na+ and K+ Transport Under Salinity Conditions</p><p>D.T. Britto and H.J. Kronzucker</p><p> </p><p>27. Trait Dissection of Salinity Tolerance with Plant Phenomics</p><p>Bettina Berger, Bas de Regt, and Mark Tester</p><p> </p><p>28. Measuring Soil Salinity</p><p>Marcus Hardie and Richard Doyle</p>
