Plant Stress Tolerance
Methods and Protocols
Samenvatting
A number of abiotic factors such as drought, salinity, extreme temperatures, low or high light intensity, and deficiency or toxic levels of nutrients have huge impacts on crop productivity, and a furthering of our understanding of the molecular, biochemical, and physiological basis of stress tolerance has been widely recognized as critical. In Plant Stress Tolerance: Methods and Protocols, expert researchers cover the most important widely-used techniques, including cutting-edge strategies, in a manner that ensures effective results. Beginning with reviews on dehydration, salinity, and cold tolerance as well as on oxidative stress, the volume then continues with methods involving topics such as describing the identification of stress-regulated genes, proteins, and microRNAs using diverse approaches, measurement of osmotic adjustment, proline levels, enzymes involved in proline metabolism, and sugars as well as determination of ROS levels, lipid peroxidation, ion leakage, and the enzymes involved in ROS detoxification. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and up-to-date, Plant Stress Tolerance: Methods and Protocols provides a wide range of easy-to-follow protocols catering to the needs of plant physiologists, biochemists, and molecular biologists interested in probing this vital area of study.
Specificaties
Inhoudsopgave
1. Dehydration Tolerance in Plants
Melvin J. Oliver, John C. Cushman, and Karen L. Koster
2. Approaches to Identifying Genes for Salinity Tolerance, and the Importance of Time Scale
Rana Munns
3. Gene Regulation During Cold Stress Acclimation in Plants
Viswanathan Chinnusamy, Jian-Kang Zhu, and Ramanjulu Sunkar
4. Redox-Dependent Regulation, Redox Control, and Oxidative Damage in Plant Cells Subjected to Abiotic Stress
Karl-Josef Dietz
5. Array Platforms and Bioinformatics Tools for the Analysis of Plant Transcriptome in Response to Abiotic Stress
Nese Sreenivasulu, Ramanjulu Sunkar, Ulrich Wobus, and Marc Strickert
6. Metabolic Engineering of Glyoxalase Pathway for Enhancing Stress Tolerance in Plants
Ananda Mustafiz, Khirod K. Sahoo, Sneh L. Singla-Pareek, and Sudhir K. Sopory
Part II: Genome-Wide Approaches for Identification of Stress Regulated Genes, Proteins, and Small RNAs
7. Genetic Screens to Identify Plant Stress Genes
Csaba Papdi, Jeffrey Leung, Mary Prathiba Joseph, Imma Perez Salamó, and László Szabados
8. Arabidopsis Tiling Array Analysis to Identify the Stress-Responsive Genes
Akihiro Matsui, Junko Ishida, Taeko Morosawa, Masanori Okamoto, Jong-Myong Kim, Yukio Kurihara, Makiko Kawashima, Maho Tanaka, Taiko Kim To, Kentaro Nakaminami, Eli Kaminuma, Takaho A. Endo, Yoshiki Mochizuki, Shuji Kawaguchi, Norio Kobayashi, Kazuo Shinozaki, Tetsuro Toyoda, and Motoaki Seki
9. Identification of Stress Responsive Genes in Plants Using Suppression Subtraction Hybridization: Ozone Stress as an Example
Lila Peal, Michael Puckette, and Ramamurthy Mahalingam
10. Identification of DNA-Binding Proteins and Protein-Protein-Interactions by Yeast One Hybrid and Yeast Two HybridScreening
Peter Klein and Karl-Josef Dietz
11. Functional Characterization of Water Deficit Stress-Induced Genes Using RNAi
Muthappa Senthil-Kumar, Makarla Udayakumar, and Kirankumar S. Mysore
12. Difference Gel Electrophoresis as a Tool to Discover Stress-Regulated Proteins
Jenny Renaut
13. Thiol-Disulfide Redox Proteomics in Plant Research
Meenakumari Muthuramalingam, Karl-Josef Dietz, and Elke Ströher
14. Cloning of Stress-Responsive MicroRNAs and other Small RNAs from Plants
Jose Luis Reyes, Catalina Arenas-Huertero, and Ramanjulu Sunkar
15. An Array Platform for Identification of Stress-Responsive MicroRNAs in Plants
Xiaoyun Jia, Venugopal Mendu, and Guiliang Tang
Part III: Antioxidant Enzymes and Metabolites
16. Spectrophotometric Assays for Antioxidant Enzymes in Plants
Sathya Elavarthi and Bjorn Martin
17. Affinity Purification and Determination of Enzymatic Activity of Recombinantly Expressed Aldehyde Dehydrogenases
Hans-Hubert Kirch and Horst Röhrig
18. Determination and Detection of Reactive Oxygen Species (ROS), Lipid Peroxidation, and Electrolyte Leakage in Plants
Niranjani Jambunathan
Part IV: Osmotic Adjustment and Ion Measurements
19. Quantification of Water Stress-Induced Osmotic Adjustment and Proline Accumulation for Arabidopsis thaliana Molecular Genetic Studies
Paul E. Verslues
20. Methods for Determination of Proline in Plants
Edit Ábrahám, Cecile Hourton-Cabassa, László Erdei, and László Szabados
21. A New Method for Accurately Measuring Delta1-Pyrroline-5-Carboxylate Synthetase Activity
Elodie Parre, Jacques de Virville, Françoise Cochet, Anne-Sophie Leprince, Luc Richard, Delphine Lefebvre-De Vos, Mohamed Ali Ghars, Marianne