Mass Spectrometry in Metabolomics
Methods and Protocols
Samenvatting
Metabolomics is a fast growing field in systems biology and offers a powerful and promising approach for a large range of applications. Metabolomics focuses on deriving the concentrations and fluxes of low molecular weight metabolites in bio-fluids, cells or tissue, plants, foods and related samples and this information provides enormous detail on biological systems and their current status. Mass Spectrometry in Metabolomics: Methods and Protocols presents a broad coverage of the major mass spectrometry (MS)-based metabolomics methods and applications. MS is one of most powerful and commonly used analytical methods in metabolomics; because so many different MS systems are used in metabolomics, this volume includes a wide variety such as triple quads, time of flight, Fourier transform ion cyclotron resonance and even simple quadrupole systems. A wide range of studies are described, with samples ranging from blood and urine to tissue and even plants. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Mass Spectrometry in Metabolomics: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further the dynamic field of metabolomics.
Specificaties
Inhoudsopgave
1. Overview: of Mass Spectrometry Based Metabolomics: Opportunities and Challenges
G. A. Nagana Gowda and Danijel Djukovic
Part II. Global LC-MS Based Metabolic Profiling
2. Global Metabolic Profiling using Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry
Yunpeng Qi, Yunlong Song, Haiwei Gu, Guorong Fan, and Yifeng Chai
3. LC-MS Profiling to Link Metabolic and Phenotypic Diversity in Plant Mapping Populations
Camilla B. Hill, Antony Bacic, and Ute Roessner
4. Mitochondrial Metabolomics Using High-Resolution Fourier-Transform Mass Spectrometry
Young-Mi Go, Karan Uppal, Douglas I. Walker, ViLinh Tran, Lauriane Dury, Frederick H. Strobel, Hélène Baubichon-Cortay, Kurt D. Pennell, James R. Roede, and Dean P. Jones
5. Sample Preparation Methods for LC-MS Based Global Aqueous Metabolite Profiling
Antoni Beltran, Sara Samino, and Oscar Yanes
Part III. Global GC-MS and CE-MS Based Metabolic Profiling
6. Methods of Discovery-Based and Targeted Metabolite Analysis by Comprehensive Two-Dimensional Gas Chromatography with Time-of-Flight Mass Spectrometry Detection
Luke C. Marney, Dr. Jamin C. Hoggard, Kristen J. Skogerboe, and Robert E. Synovec
7. Analysis of Mouse Liver Metabolites by GC×GC–TOF MS
Xue Shi, Xinmin Yin, and Xiang Zhang
8. Metabolite Fingerprinting by Capillary Electrophoresis-Mass Spectrometry
Antonia Garcia, Shama Naz, and Coral Barbas
Part IV. Isotope Labeling Methods
9. Quantitative Metabolomic Profiling using Dansylation Isotope Labeling and Liquid Chromatography Mass Spectrometry
Ruokun Zhou and Liang Li
10. Quantitative Analysis of Amino and Organic Acids by Methyl Chloroformate Derivatization and GC-MS/MS Analysis
Hans Fredrik Nyvold Kvitvang, Kåre A. Kristiansen, Stina K. Lien, and Per Bruheim
11. Stable Isotope Labeled Tracers for Metabolic Pathway Elucidation by GC-MS and FT-MS
Richard M. Higashi, Teresa W-M. Fan, Pawel K. Lorkiewicz, Hunter N.B. Moseley, and Andrew N. Lane
Part V. Targeted Aqueous Metabolite Profiling
12. Multiplexed, Quantitative and Targeted Metabolite Profiling by LC-MS/MRM
Ru Wei, Guodong Li, and Albert B. Seymour
Part VI. Lipid Profiling Methods
13. Multi-Dimensional Mass Spectrometry-Based Shotgun Lipidomics
Miao Wang and Xianlin Han
14. Comprehensive Quantitative Determination of PUFA-Related Bioactive Lipids for Functional Lipidomics using High Resolution Mass Spectrometry
Mojgan Masoodi and Dietrich A. Volmer
15. Ultra-Performance Liquid Chromatography Mass Spectrometry Targeted Profiling of Bile Acids: Application to Serum, Liver Tissue and Cultured Cells of Different Species
Juan Carlos García-Cañaveras, María Teresa Donato, and Agustín Lahoz
Part VII. Profiling Volatile Organic Compounds
16. Analysis of Volatile Organic Compounds in Exhaled Breath by Gas Chromatography-Mass Spectrometry combined with Chemometric Analysis
Jan W. Dallinga, Agnieszka Smolinska, and Frederik-Jan van Schooten
17. Headspace SPME-GC-MS Metabolomics Analysis of Urinary Volatile Organic Compounds (VOCs)
Shucha Zhang and Daniel Raftery
Part VIII. Profiling and Imaging using Atmospheric Sampling Methods
18. Metabolite Profiling by Direct Analysis in Real Time Mass Spectrometry
Christina M. Jones, María Eugenia Monge, and Facundo M. Fernández
19. Analysis of Dried Blood Spots using DESI Mass Spectrometry
Justin M. Wiseman and Joseph H. Kennedy
20. DESI-MS Imaging of Lipids and Metabolites from Biological Samples
Livia S. Eberlin
21. Metabolic Imaging using Nanostructure-Initiator Mass Spectrometry (NIMS)
Katherine B. Louie and Trent R. Northen
Part IX. Statistical Analysis Methods
22. Statistical Analysis and Modeling of Mass Spectrometry-Based Metabolomics Data
Bowei Xi, Haiwei Gu, Hamid Baniasadi, and Daniel Raftery
