José Luis Riechmann,
Frank Wellmer
Springer New York
e druk, 2016
9781493943401
Flower Development
Methods and Protocols
Specificaties
Paperback, blz.
|
Engels
Springer New York |
e druk, 2016
ISBN13: 9781493943401
Rubricering
Onderdeel van serie
Methods in Molecular Biology
Verwachte levertijd ongeveer 9 werkdagen
Samenvatting
In Flower Development: Methods and Protocols, researchers in the field detail protocols for experimental approaches that are currently used to study the formation of flowers, from genetic methods and phenotypic analyses, to genome-wide experiments, modeling, and system-wide approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls
Authoritative and practical, Flower Development: Methods and Protocols is an essential guide for plant developmental biologists, from the novice to the experienced researcher, and for those considering venturing into the field.
Specificaties
ISBN13:9781493943401
Taal:Engels
Bindwijze:paperback
Uitgever:Springer New York
Inhoudsopgave
<p>Part I: Review and Overview Chapters</p><p>1. Flower Development in Arabidopsis - There is More to it than Learning your ABCs</p><p>Nathanael Prunet and Thomas Jack</p><p> </p><p>2. Flower Development in the Asterid Lineage</p><p>Barry Causier and Brendan Davies</p><p> </p><p>3. Grass Flower Development</p><p>Hiro-Yuki Hirano, Wakana Tanaka, and Taiyo Toriba</p><p> </p><p>4. Flower Diversity and Angiosperm Diversification</p><p>Pamela S. Soltis and Douglas E. Soltis</p><p> </p><p>5. Flower Development - Open Questions and Future Directions</p><p>Frank Wellmer, John L. Bowman, Brendan Davies, Cristina Ferrándiz, Jennifer C. Fletcher, Robert G. Franks, Emmanuelle Graciet, Veronica Gregis, Toshiro Ito, Thomas P. Jack, Yuling Jiao, Martin M. Kater, Hong Ma, Elliot M. Meyerowitz, Nathanael Prunet, José Luis Riechmann<sup></sup></p><p> </p><p>PART II: Genetic and Phenotypic Analyses</p><p>6. Genetic Screens for Floral Mutants in Arabidopsis thaliana: Enhancers and Suppressors</p><p>Thanh Theresa Dinh, Elizabeth Luscher, Shaofang Li, Xigang Liu, So Youn Won, and Xuemei Chen<sup></sup></p><p> </p><p>7. Genetic and Phenotypic Analysis of Shoot Apical and Floral Meristem Development</p><p>Mona M. Monfared and Jennifer C. Fletcher</p><p> </p><p>8. Genetic and Phenotypic Analyses of Petal Development in Arabidopsis</p><p>Judit Szécsi, Barbara Wippermann and Mohammed Bendahmane </p><p> </p><p> </p><p>9. Cell Biological Analyses of Anther Morphogenesis and Pollen Viability in Arabidopsisand Rice</p><p>Fang Chang, Zaibao Zhang, Yue Jin, and Hong Ma<sup> </sup></p><p> </p><p>10. Molecular Cell Biology of Male Meiotic Chromosomes and Isolation of Male Meiocytes in Arabidopsis thaliana</p><p>Yingxiang Wang, Zhihao Cheng, Pingli Lu, Ljudmilla Timofejeva, Hong Ma</p><p> </p><p>11. Genetic and Phenotypic Analyses of Carpel Development in Arabidopsis </p><p>Vicente Balanzà, Patricia Ballester, Monica Colombo, Chloé Fourquin, Irene Martínez-Fernández, and Cristina Ferrándiz</p><p> </p><p> </p><p> </p><p>Part III: Microscopy and Histology</p><p>12. Microscopic Analysis of Arabidopsis Ovules</p><p>Balaji Enugutti and Kay Schneitz</p><p> </p><p>13. Scanning Electron Microscopy Analysis of Floral Development</p><p>Robert G. Franks </p><p> </p><p>14. Detection of mRNA Expression Patterns by Non-radioactive in situ Hybridization on Histological Sections of Floral Tissue</p><p>Anna Medzihradszky, Kay Schneitz, and Jan U. Lohmann</p><p> </p><p>15. The GUS Reporter System in Flower Development Studies</p><p>Janaki S. Mudunkothge and Beth A. Krizek</p><p> </p><p> </p><p>Part IV: Experimental Systems</p><p>16. A Floral Induction System for the Study of Early Arabidopsis Flower Development</p><p>Diarmuid Ó’Maoiléidigh and Frank Wellmer</p><p> </p><p>17. Fluorescence Activated Cell Sorting of Shoot Apical Meristem Cell Types</p><p>G. Venugopala Reddy</p><p> </p><p>18. Translating Ribosome Affinity Purification (TRAP) for Cell-specific Translation Profiling in Developing Flowers </p><p>Ying Wang and Yuling Jiao</p><p> </p><p>19. Laser-assisted Microdissection Applied to Floral Tissues</p><p>Samuel E. Wuest and Ueli Grossniklaus</p><p> </p><p>PartV: Molecular Biology, Genomics, and Systems Biology</p><p> </p><p>20. Identification of Arabidopsis Knock-out Lines for Genes of Interest</p><p>José Tomás Matus, Thilia Ferrier, and José Luis Riechmann</p><p> </p><p>21. Gene Expression Analysis by Quantitative Real-time PCR for Floral Tissues</p><p>Mariana Bustamante, Jian Jin, Oriol Casagran, Tania Nolan, and José Luis Riechmann<sup></sup></p><p> </p><p>22. Misexpression Approaches for the Manipulation of Flower Development </p><p>Yifeng Xu, Eng-Seng Gan, and Toshiro Ito<sup></sup></p><p> </p><p>23. Next Generation Sequencing Applied to Flower Development: RNA-Seq</p><p>Jun He and Yuling Jiao</p><p> </p><p>24. Next-Generation Sequencing Applied to Flower Development: ChIP-Seq</p><p>Emmanuelle Graciet, Diarmuid Seosamh Ó'Maoiléidigh, and Frank Wellmer</p> 25. Live-Imaging of the Arabidopsis Inflorescence MeristemMarcus G. Heisler<sup> </sup>and Carolyn Ohno<p> </p><p>26. Gene Regulatory Network Models for Floral Organ Determination</p><p>Eugenio Azpeitia, José Davila-Velderrain, Carlos Villarreal, and Elena R. Alvarez-Buylla</p><p> </p>