<p>1. Method for Assaying the Lipid Kinase Phosphatidylinositol-5-phosphate 4-kinase α in Quantitative High-Throughput Screening (qHTS) Bioluminescent Format</p><p>Mindy I. Davis, Atsuo T. Sasaki, and Anton Simeonov</p><p> </p><p>2. Assaying Ceramide Synthase Activity In Vitro and in Living Cells using Liquid Chromatography-Mass Spectrometry</p><p>Xin Ying Lim, Russell Pickford, and Anthony S Don</p><p> </p><p>3. Fluorescent Assays for Ceramide Synthase Activity</p><p>Timothy A Couttas and Anthony S Don</p><p> </p><p>4. Identification of the Interactome of a Palmitoylated Membrane Protein, Phosphatidylinositol 4-kinase type II Alpha</p><p>Avanti Gokhale, Pearl V. Ryder, Stephanie A. Zlatic, and Victor Faundez</p><p> </p><p>5. Measurement of Long Chain Fatty Acyl-CoA synthetase Activity</p><p>Joachim Füllekrug and Margarete Poppelreuther</p><p> </p><p>6. Qualitative and Quantitative in vitro Analysis of Phosphatidylinositol Phosphatase Substrate Specificity</p><p>Laura Ren Huey Ip and Christina Anja Gewinner</p><p> </p><p>7. Luciferase Reporter Assays to Assess Liver X Receptor Transcriptional Activity</p><p>Matthew C. Gage, Benoit Pourcet, and Inés Pineda-Torra</p><p> </p><p>8. Metabolically Biotinylated Reporters for Electron Microscopic Imaging of Cytoplasmic Membrane Microdomains</p><p>Kimberly J. Krager and John G. Koland</p><p> </p><p>9. Fluorescence Recovery after Photobleaching Analysis of the Diffusional Mobility of Plasma Membrane Proteins: HER3 Mobility in Breast Cancer Cell</p> Membranes<p></p><p>Mitul Sarkar and John G. Koland</p><p> </p><p>10. Isolation and Analysis of Detergent-Resistant Membrane Fractions</p><p>Massimo Aureli, Sara Grassi, Sandro Sonnino, and Alessandro Prinetti</p><p> </p><p>11. Detection of Isolated Mitochondria-Associated ER Membranes using the Sigma-1 Receptor</p><p>Abasha Lewis and Tsung-Ping Su</p><p> </p><p>12. Using Surface Plasmon Resonance to Quantitatively Assess Lipid-Protein Interactions</p><p>Kathryn Del Vecchio and Robert V. Stahelin</p><p> </p><p>13. Analyzing Protein-phosphoinositide Interactions with Liposome Flotation Assays</p><p>Ricarda A. Busse, Andreea Scacioc, Amanda M. Schalk, Roswitha Krick, Michael Thumm, and Karin Kühnel</p><p> </p><p>14. High-Throughput Flurometric Assay for Membrane-Protein Interaction</p><p>Wonhwa Cho, Hyunjin Kim, and Yusi Hu</p><p> </p><p>15. Guidelines for the use of Protein Domains in Acidic Phospholipid Imaging</p><p> Matthieu Pierre Platre and Yvon Jaillais</p><p> </p><p>16. Analysis of Sphingolipid Synthesis and Transport by Metabolic Labeling of Cultured Cells with [<sup>3</sup>H]serine </p><p>Neale D. Ridgway </p><p> </p><p>17. Determination and Characterization of Tetraspanin-Associated Phosphoinositide-4 Kinases in Primary and Neoplastic Liver Cells </p><p>Krista Rombouts and Vinicio Carloni</p><p> </p><p>18. Analysis of the Phosphoinositide Composition of Subcellular Membrane Fractions</p><p>Deborah A. Sarkes and Lucia E. Rameh </p><p> </p><p>19. Single-molecule</p> Imaging of Signal Transduction via GPI-anchored Receptors<p></p><p>Kenichi G. N. Suzuki<sup></sup></p><p> </p><p>20. Measuring Phosphatidylinositol Generation on Biological Membranes</p><p>Mark G. Waugh</p><p> </p><p>21. Assay for CDP-diacylglycerol Generation by CDS in Membrane Fractions</p><p>Mark G. Waugh</p><p> </p><p> </p>
