RNA Nanotechnology and Therapeutics

<p>Overview of Methods in RNA Nanotechnology - Synthesis, Purification and Characterization of RNA Nanoparticles.- Multiple Approaches for the Investigation of Bacterial Small Regulatory RNAs Self-Assembly.- Measuring the Elasticity of Ribonucleotide(s)-Containing DNA Molecules Using AFM.- Silver Nano clusters for RNA Nano technology: Steps Towards Visualization and Tracking of RNA Nanoparticle Assemblies.- Purification of RNA Nanoparticles by Ultracentrifugation.- HPLC Purification of RNA Aptamers up to 59 Nucleotides with Single Nucleotide Resolution.- RNA Nanoparticles with Thermostable Motifs and Fluorogenic Modules for Real-Time Detection of RNA Folding and Turnover In Vivo.- Fluorescence Labeling of Short RNA by Oxidation at the 3’-End.- Methods and Assays for Specific Targeting and Delivery of RNA Nanoparticles to Cancer Metastases.- Functional Assays for Specific Targeting and Delivery of RNA Nanoparticles to Brain Tumor.- Aptamer-Mediated Nanoparticle Interactions: From Oligonucleotide-Protein Complexes to SELEX Screens.- Methods for Assembling B-Cell Lymphoma Specific and Internalizing Aptamer-siRNA Nanoparticles Via the Sticky Bridge.- A High Throughput Screening Assay for the Functional Delivery of Splice-Switching Oligonucleotides in Human Melanoma Cells.- Design, Assembly, and Evaluation of RNA-Protein Nanostructures.- Mapping RNA Interactions to Proteins in Virions Using CLIP-Seq.- Mapping Protein-RNA Interactions by RCAP, RNA-Crosslinking and Peptide Fingerprinting.</p>