Proteins

A. Methods of Polypeptide Purification and Characterization.- I. Chromatographic Methods of Polypeptide Purification.- Use of Short HPLC Column For The Concentration, Separation and Recovery of Subnanomole Amounts of Protein and Polypeptides For Microsequence Analysis.- Practical and Theoretical Aspects of Microbore HPLC of Proteins, Peptides and Amino Acid Derivatives.- Microisolation and Sequence Analysis of Human Epidermal Growth Factor.- Complete Amino Acid Sequence Determination of Rat Epidermal Growth Factor:Characterization of a Truncated Form With Full In Vitro Biological Activity.- Use of HPLC Comparative Peptide Mapping in Structure/ Function Studies.- Precolumn Derivatization of Peptides With Phenlisothiocynate (PITC).- High-Performance Liquid Chromatography as a Means of Characterizing Isoforms of Steroid Hormone Receptor Proteins.- Large Scale Purification of a Human Hepatoma Derived Endothelial Cell Growth Factor.- Very Rapid Microanalysis of IgG in Ascites Fluids By HPLC Using a Novel Anion-Exchange Column.- Application of Immunoaffinity Chromatography to the Purification of Polypeptides for Microsequence Analysis.- II. Electrophortic Methods of Polypeptide Purification.- A Novel Approach to Isolation of Proteins for Micro-sequences Analysis:Electroblotting onto Activated Glass.- Blotting of Protein-Detergent Complexes on Glass and Glass Coated with Polybases:A Comparative Study. Acid Hydrolysis and Gas-Phase Sequencing of Gel- Separated Proteins Immobilized on Polybrene Glass Fiber.- Two-Dimensional Gel Electrophoresis of Polypeptides.- III. Chemical Modification of Polypeptides.- Carboxymethylated Hemoglobin as a Structural Analogue For Carbamino Hemoglobin.- Gossypol:Interaction with Ribonuclease A.- Studies on Nonenzymic Glycosylation of Peptides in a Simple Model System.- Reaction of Glyceraldehyde (Aldotriose) with Proteins is a Prototype of Nonenzymic Glycation:Protein Cross-linking as a Consequence of In Vitro Non-Enzymic Glycation.- Reversible Dihydroxypropylation of Amino Groups in Proteins:Application in Primary Structural Studies of Streptococcal M-Proteins.- Enzymatic Carboxylmethylation and Non-Enzymatic Demethylation at L-Isoaspartyl Residues:Possible Applications in Peptide Chemistry.- IV. Amino Acid Analysis of Polypeptides.- A Practical Guide to the General Application of PTC-Amino Acid Analysis.- High Performance Liquid Chromatography of Phenylthiodantoin and Phenylthiocarbamyl Amino Acids.- Amino Acid Analysis Using Pre-Column Derivatization with Phenylisothiocyanate:Matrix Effects and Tryptophan Analysis.- An Improved Reverse Phase HPLC Separation and Post-Column Detection of Amino Acids.- HPLC Assay of Phosphoamino Acids by Fluorescence Detection.- Amino Acid Analysis by Reverse-Phase High Performance Liquid Chromatography:Separation of Phenylthiocarbamyl Amino Acids by Spherisorb Octadecylsilane Columns.- V. Mass Spectrometry of Polypeptides.- Fast Atom Bombardment Mass Spectrometry:Application to Peptide Structural Analysis.- Protein Sequence Determination and FAB Mass Spectrometry.- The Use of HPLC and Mass Spectrometric Techniques to Quantify Endogenous opioid peptides.- Structural Analysis of Proteins of the Nervous System.- Pancreatic Preprosomatostatin Processing:Isolation and Structure of Intermediates and Final Products of Processing.- VI. Microsequence Analysis of Polypeptides by Edman Degraduation.- Progress Toward Polybrene Purification and Utility in Micro-Peptide/Protein Sequencing.- Approaches for Microsequencing:Extraction from SDS gel, Composition, Terminal Analysis.- Manual Gas Phase Microsequencing of Filterchips for Simultaneous Edman Degradation of Multiple Peptide Samples.- Strategies For Sequencing Peptide Mixtures by Selective Blocking.- Amidatioti of Protein Carboxyl Groups.- A Method for Direct Amino Acid Sequence Analysis of the NH2-Terminal Regions of Fibrinogen.- VII. Analysis of PTH Amino Acids.- PTH Amino Acid Analysis.- An On-Line Isocratic HPLC System for the Analysis of PTH-Amino Acids on a Gas-Phase Sequencer.- Routine Analysis of Low-Picomole-Level Phenylthiohy-dantoins by HPLC Using a Diisopropylethylamine Acetate/THF Buffer and Acetonitrile Gradient.- Identification of Side-Chain Protected L -Phenylthiohy- dantoins on Cyano HPLC Columns:An Application to Gas-Phase Microsequencing of Peptides Synthesized on Solid-Phase Supports.- Separation of Amino Acid Phenylthiohydantoin Derivatives by High Pressure Liquid Chromatography.- VIII. Computer Analysis of Protein Sequence Data.- Hydrophobic Moments as Tools for Analysis of Protein Sequences and Structures.- Identification of Protein Surfaces and Interaction Sites by Hydrophilicity Analysis.- The Protein Identification Resource (PIR):An On-Line Computer System for the Characterization of Proteins Based on Comparisons with Previously Characterized Protein Sequences.- Computer Analysis of Protein Sequencing Data.- IX. Miscellaneous Methods of Polypeptide Characterization.- Simultaneous Multiple Peptide Synthesis:The Rapid Preparation of Large Numbers of Peptides.- Mapping Functional Domains of Human Platelet Thrombo-spondin with Electroblotting and High Sensitivity Sequencing.- Immunoprotection - A Novel Approach for Mapping Epitopes on an Antigen.- Micro Method for the Detection of Heparin-Binding Proteins and Peptides.- Rapid and Sensitive Determination of Protein Disulfide Bonds.- Simulation of Protein Hydrodynamic Changes Observed by Urea Gradient Gel Electrophoresis.- B. Analysis of Polypeptide Structure and Function.- X. Site-Directected Mutagenesis.- Site-Specific Mutations in Dihydrofolate Reductase at the Dihydrofolate Binding Site.- Site Saturation Mutagenesis of Active Site Residues of ß-Lactamase.- Enzyme Thermostability and its Enhancement by Genetic Engineering.- Expression of Human C5a in E. coli.- XI. Active Site Studies.- Use of Trinitrobenzene Sulfonate to Determine the pKa Values of Two Active-Site Lysines of Ribulose-bisphosphate Carboxylase/Oxygenase..- Advances in Affinity Labeling of Purine Nucleotide Sites in Dehydrogenases.- Escherichia Coli ADPglucose Synthetase Subtrate Inhibitor Binding Sites Studied by Site(s) Directed Chemical Modification and Mutant Enzyme Characerization.- Active Site and Other Sequence Data from Torpedo Californica Acetylcholinesterase.- Acetylcholine Receptor a-Bungarotoxin Interactions Studied by Chemical Modification, Enzyme Digestion and HPLC.- Imidoesters and the Mechanism of Liver Alcohol Dehydrogenase.- Investigation of Gramicidin Channel Function by Single Amino Acid Replacement Using Non-Genetic Code Amino Acids.- XII. Domain and Topographical Studies.- Identification of Functional Domains of the Inhibitor Protein of cAMP-Dependent Protein Kinase.- Atrial Natriuretic Factor Receptor in Adrenal Plamsa Membrane:Identification by Photo-Affinity Labeling.- Structual Analysis of a 29/38kDa Heparin-Binding Domain of Fibronectin:Evidence That Two Different Subunits of Human Plamsa Fibronectin Arise by Alternative mRNA Splicing.- Structural and Functional Studies on the Purified Insulin Receptor.- Localization and Interaction of Functional Sites on Antithrombin III. Use of an Anti-Hapten Antibody as a Structural Probe.- XIII. Characterization of Proteases.- Properties of the Hydrolase that Catalyzes Removal of the Blocked NH2-Terminal Amino Acid Residues From Polypeptides.- Kinetic Product Analysis of Aspartyl Proteinases Utilizing New Synthetic Substrates and Reversed Phase HPLC.- The Esterase-Like Activity of Covalently Bound C3.- XIV. Identification of Sites of Post-Translateral Modification.- Structural Analysis of Carcinoembryonic Antigen (CEA) and a Related Tumor-Associated Antigen (TEX).- Determination of the Location of NG,NGDimethylarginine in a Glycine-Rich Region of Nucleolar Protein C23.- Structural Characterization of a Murine Lymphocyte Homing Receptor Suggests a Ubiquitinated Branched-Chain Glycoprotein.- XV. New Sequences.- Human C3b/C4b Receptor (CR1):Isolation, Protein Sequence Analysis, and Cloning of a Partial cDNA From Human Tonsil.- HTLV-III/LAV Particle-Associated Proteins:I. Virus Purification, Inactivation and Basic Characterization.- Structure and Function of Fish Antifreeze Polypeptides.- Sequence of a Glycine-Rich Protein From Lizard Claw:Unusual Dilute Acid and Heptafluorobutyric Acid Cleavages.- Primary Structure Studies on Serine Hydroxymethyltransferase.- The Proteins in Avian Eggs:A Test of Protein Separation Methods Based on HPLC.- Amino Terminal Sequence of 7B2:A Novel, Human Pituitary/Brain Polypeptide Present in Gonadotrophs and Released by LHRH.- C. Summary of Symposium Workshop.- The Syposium Test Peptide (STP):Synthesis and Characterization of a Model Peptide.- Workshop in Microanalytical Methods - Experiences With an Unknown Synthetic Peptide.- Contributors.