Forensic DNA Analysis

Methods and Protocols

Specificaties
Gebonden, blz. | Engels
Springer US | e druk, 2023
ISBN13: 9781071632949
Rubricering
Springer US e druk, 2023 9781071632949
Onderdeel van serie Methods in Molecular Biology
€ 276,99
Levertijd ongeveer 8 werkdagen

Samenvatting

This volume focuses on the latest techniques used in forensic DNA analysis. The chapters include a comprehensive collection of extraction, quantification, STR amplification, and detection methods for routine forensic samples, including manual, semi-automated, and automated procedures using both home-brew and commercial products. The chapters also discuss probabilistic modeling software and specialized start-to-finish procedures for mitochondrial DNA analysis, archived latent fingerprints, latent DNA, rapid DNA profiling, and next-generation sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Forensic DNA Analysis: Methods and Protocols is a valuable resource for researchers interested in learning more about forensic DNA analysis procedures.

Specificaties

ISBN13:9781071632949
Taal:Engels
Bindwijze:gebonden
Uitgever:Springer US

Inhoudsopgave

<div>Forensic DNA Analysis: An Overview of the Laboratory Process.-&nbsp;Organic Extraction of Nucleic Acids using Ethanol Precipitation or Microcon® Centrifugal Filter Purification Methods.-&nbsp;Manual Silica-Based DNA Extractions.-&nbsp;Applied Biosystems™ PrepFiler™ Forensic DNA Extraction Kit (Manual and Semi-Automated via AutoMate Express™).-&nbsp;Robotic DNA Extraction Utilizing Qiagen BioSprint® 96 Workstation.-&nbsp;DNA Extraction of Bone through Demineralization.-&nbsp;Differential Extraction with Purification via Organic/Microcon® and Promega DNA IQ™ Methods.-&nbsp;DNA Purification from Bloodstains and Buccal Cells/Saliva on FTA® Cards.-&nbsp;Yield Gel via Quantitative Gel Electrophoresis.-&nbsp;Quantitative PCR of Alu Repeats using PowerUp™ SYBR® Green Master Mix.-&nbsp;Quantitation of DNA using the Applied Biosystems Quantifiler® Trio DNA Quantification Kit.-&nbsp;QIAGEN's Investigator® Quantiplex Pro Kit.-&nbsp;DNA Amplification using Promega's PowerPlex® Fusion Systems (5C and 6C).-&nbsp;Amplification of Extracted DNA and Direct Amplification with the PowerPlex® Y23 System.-&nbsp;Applied Biosystems' GlobalFiler™ PCR Amplification Kit.-&nbsp;QIAGEN’s Investigator® 24plex QS and GO! PCR Amplification.-&nbsp;Low Volume STR Amplification Options: Coupling with Standard or Fast PCR, Traditional or Normalized DNA Extraction, and/or Traditional or Alternative Capillary Electrophoresis.-&nbsp;Capillary Electrophoresis with Applied Biosystems' 3500 Genetic Analyzer.-&nbsp;Likelihood Ratio Calculation using LRmix Studio.-&nbsp;Mitochondrial DNA Analysis.-&nbsp;An Optimized Forensic DNA Analysis Workflow for Obtaining STR Results from Archived Latent Fingerprints.-&nbsp;Detection of Latent DNA using a DNA Binding Dye.-&nbsp;Rapid DNA Profile Development with Applied Biosystems RapidHIT™ ID System.-&nbsp;Next-Generation Sequencing: ForenSeq™ DNA Signature Prep Kit with the Illumina MiSeq FGx.</div><br><div><br></div>
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        Forensic DNA Analysis