Contemporary Approaches to the Study of Pain

From Molecules to Neural Networks

Specificaties
Paperback, blz. | Engels
Springer US | e druk, 2023
ISBN13: 9781071620410
Rubricering
Springer US e druk, 2023 9781071620410
Onderdeel van serie Neuromethods
€ 244,99
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Samenvatting

This volume contains experimental approaches that are currently revolutionizing our understanding of the neurobiology of pain. The chapters cover many cutting-edge methods including the identification of gene expression profiles, transcriptomes or translatomes, from individual cells or defined groups of cells in rodents and primates;  the electrophysiological investigation of human tissues, such as human dorsal root ganglion neurons; ways to assess modality response profiles of neurons using calcium imaging in vitro and in vivo; and somatosensory behaviors in rodents using high-speed videography and machine learning.  In the Neuromethods series style, the chapters include detailed advice from specialists to obtain successful results in your laboratory.

Cutting-edge and comprehensive, Contemporary Approaches to the Study of Pain: From Molecules to Neural Networks is a valuable resource for scientists and researchers interested in making impactful contributions to our understanding of pain.

Specificaties

ISBN13:9781071620410
Taal:Engels
Bindwijze:paperback
Uitgever:Springer US

Inhoudsopgave

Single-Cell RNA-Sequencing of Somatosensory Neurons.-&nbsp;Preparation of Human and Rodent Spinal Cord Nuclei for Single-Nucleus Transcriptomic Analysis.-&nbsp;Multiplex In Situ Hybridization of the Primate and Rodent DRG and Spinal Cord.-&nbsp;Using Translating Ribosome Affinity Purification (TRAP) to Understand Cell-Specific Translatomes in Pain States.-&nbsp;Ex Vivo Skin-Teased Fiber Recordings from Tibial Nerve.-&nbsp;Single Unit Electrophysiological Recordings of Primary Muscle Sensory Neurons using a Novel Ex Vivo Preparation.-&nbsp;Electrophysiological Recording Techniques from Human Dorsal Root Ganglion.-&nbsp;Human Pluripotent Stem Cell-Derived Sensory Neurons: A New Translational Approach to Study Mechanisms of Sensitization?.-&nbsp;Intraspinal Transplantation of Precursors of Cortical GABAergic Interneurons to Treat Neuropathic Pain.-&nbsp;A Co-Culture System for Studying Dorsal Spinal Cord Synaptogenesis.-&nbsp;Visualizing Synaptic Connectivity using Confocal and Electron Microscopy: Neuroanatomical Approaches to Define Spinal Circuits.-&nbsp;Using Viral Vectors to Visualize Pain-Related Neural Circuits in Mice.-&nbsp;Recording Pain-Related Brain Activity in Behaving Animals using Calcium Imaging and Miniature Microscopes.-&nbsp;Optical Imaging of the Spinal Cord for the Study of Pain: From Molecules to Neural Networks.-&nbsp;In Vivo Calcium Imaging of Peripheral Ganglia.-&nbsp;Optogenetic Modulation of the Visceromotor Response to Reveal Visceral Pain Mechanisms.-&nbsp;Use of Optogenetics for the Study of Skin-Nerve Communication.-&nbsp;Channelrhodopsin-2 Assisted Circuit Mapping in the Spinal Cord Dorsal Horn.-&nbsp;Production of AAVs and Injection into the Spinal Cord.-&nbsp;The Use of Intraspinally Injected Chemogenetic Receptor, PSAM-GlyR, to Probe the Functional Role of Spinal Dorsal Horn Neurons.-&nbsp;Measuring Mouse Somatosensory Reflexive Behaviors with High-Speed Videography, Statistical Modeling, and Machine Learning.<div><br></div>
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        Contemporary Approaches to the Study of Pain