RNAi Strategies for Pest Management

Methods and Protocols

Specificaties
Paperback, blz. | Engels
Springer US | e druk, 2022
ISBN13: 9781071616352
Rubricering
Springer US e druk, 2022 9781071616352
Onderdeel van serie Methods in Molecular Biology
Verwachte levertijd ongeveer 9 werkdagen

Samenvatting

This volume explores the latest techniques used for sustainable development of eco-friendly pest management systems. Chapters in this book look at the design of novel RNAi-mediated pest control strategies and related issues. Some of the topics covered in this book are identification and characterization of differentially expressed non-coding RNAs in insect genomes; functional small RNA design; analysis of endogenous RNAi pathways; RNAi screening; plant models of RNAi-mediated pest control; and dsRNA delivery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Cutting-edge and comprehensive, RNAi Strategies for Pest Management: Methods and Protocols is a valuable resource for any scientist or researcher interested in learning more about this exciting and developing field. 
Chapter 18 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Specificaties

ISBN13:9781071616352
Taal:Engels
Bindwijze:paperback
Uitgever:Springer US

Inhoudsopgave

<div>Genome-Wide Identification and Functional Characterization of Non-Coding RNAs (ncRNAs) Differentially Expressed during Insect Development.-&nbsp;Identification and Functional Characterization of Argonaute (Ago) Proteins in Insect Genomes.-&nbsp;Functional Characterization of Silkworm PIWI Proteins by Embryonic RNAi.-&nbsp;Guiding RNAi Design through Characterization of Endogenous Small RNA Pathways.-&nbsp;Silencing of Molecular Targets with Relevance to Insecticide Resistance in Colorado Potato Beetle using dsRNA.-&nbsp;Visualization of RNA Transcripts in Western Corn Rootworm (Diabrotica virgifera virgifera) and Plants by In Situ Hybridization.-&nbsp;In Silico Screening of Attractive Target Genes for RNAi-Based Pest Control.-&nbsp;RNAi Feeding Bioassay: A Protocol for dsRNA Screening against Asian Citrus Psyllid and Related Hemipteran Insects.-&nbsp;A Random-Screening Approach to Identify RNAi Targets for the Control of Western Corn Rootworm (Diabrotica. virgifera virgifera Le Conte).-&nbsp;Rapid Screening of Myzus persicae (Green Peach Aphid) RNAi Targets using Tobacco rattle virus.-&nbsp;Genome-Wide Identification and Validation of Target Genes Associated with Insecticide Treatment of the Green Peach Aphid, Myzus persicae.-&nbsp;Inhibition of Rhopalosiphum maidis (Corn Leaf Aphid) Growth on Maize by Virus-Induced Gene Silencing with Sugarcane mosaic virus.-&nbsp;RT-qPCR for Detection and Expression Monitoring of Core Genes Involved in the RNA Interference Pathways of Western Corn Rootworm (Diabrotica virgifera virgifera).-&nbsp;Examination of the Suitability of Attractive Target Genes for RNAi-Based Pest Control.-&nbsp;Functional Characterization of Target Genes Associated with Insecticide Resistance of the Green Peach Aphid, Myzus persicae.-&nbsp;Plant-Mediated RNA Interference Expressing dsRNA in Cytoplasm for RNAi-Based Pest Control.-&nbsp;RNAi Expression in Cotton for Control of Herbivorous Insects.-&nbsp;Transplastomic Tomato Plants Expressing Insect-Specific Double-Stranded RNAs: A Protocol Based on Biolistic Transformation.-&nbsp;Double-Strand RNA (dsRNA) Delivery Methods in Insects: Diaphorina citri.-&nbsp;Delivery of Double-Stranded RNAs (dsRNAs) Produced by Escherichia coli HT115 (DE3) for Non-Transgenic RNAi-Based Insect Pest-Management.-&nbsp;Symbiont-Mediated RNA Interference (SMR): Using Symbiotic Bacteria as Vectors for Delivering RNAi to Insects.-&nbsp;Application of Nanoparticle-Mediated RNAi for Efficient Gene Silencing and Pest Control on Soybean Aphids.-&nbsp;Methods for Delivery of dsRNAs for Agricultural Pest Control: The Case of Lepidopteran.-&nbsp;The CRISPR/Cas9-Mediated Genome Editing System in Insect Genomics and Pest Management.</div><div><br></div>

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        RNAi Strategies for Pest Management