Small Non-Coding RNAs

Methods and Protocols

Specificaties
Paperback, blz. | Engels
Springer US | 2e druk, 2022
ISBN13: 9781071613887
Rubricering
Springer US 2e druk, 2022 9781071613887
Onderdeel van serie Methods in Molecular Biology
€ 138,99
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Samenvatting

This second edition provides new and updated chapters detailing methods tackling all aspects of small non-coding RNAs biology.  Chapters guide readers through customized dedicated protocols and technologies that will be of valuable help decipher the numerous functions of small non-coding RNAs. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on  troubleshooting and avoiding known pitfalls.

Instructive and practical, Small Non-Coding RNAs: Methods and Protocols, Second Edition aims to be a valuable help and of great interest to a large number of researchers working in the field of RNA or willing to develop RNA studies.

Specificaties

ISBN13:9781071613887
Taal:Engels
Bindwijze:paperback
Uitgever:Springer US
Druk:2

Inhoudsopgave

Regulatory Non-coding RNAs: An Overview.-&nbsp;RNA Precipitation.-&nbsp;Quantitative and Qualitative Assessment of Small RNA Preparations.-&nbsp;Non-coding RNA Silencing in Mammalian Cells by Antisense LNA GapmeRs Transfection.-&nbsp;Northern Blot Detection of Tiny RNAs.-&nbsp;Stem-loop qRT-PCR Based Quantification of miRNAs.-&nbsp;Detection and Labeling of Small Non-coding RNAs by Splinted Ligation.-&nbsp;Fluorescence In Situ Hybridization Of Small Non-coding RNAs.-&nbsp;Using native RIP, UV-CLIP or fCLIP to Address Protein-RNA Interactions In Vivo.-&nbsp;Purification of RiboNucleoProtein Particles by MS2-MBP Affinity Chromatography.-&nbsp;Study of genome-wide occupancy of long non-coding RNAs using Chromatin Isolation by RNA Purification (ChIRP).-&nbsp;Gene Reporter Assays to Study miRNA Function.-&nbsp;<strong>CRISPR/Cas9 System to Knock-Down microRNA </strong>In Vitro <strong>and </strong>In Vivo.-&nbsp;Microarray Analysis of Whole-Transcriptome RNAs including Non-Coding RNAs.-&nbsp;Isolation, Extraction, and Deep-Sequencing Analysis Of Extracellular RNAs (exRNAs) from Human Plasma.-&nbsp;Analysis of the RNA and protein complexome by Grad-seq.-&nbsp;µIVC-Seq: A Method for Ultrahigh-throughput Development and Functional Characterization of Small RNAs.-&nbsp;RNA Secondary Structure Study by Chemical Probing Methods using DMS and CMCT.-&nbsp;Application of NMR Spectroscopy to Determine the 3D structure of Small Non-coding RNAs.<p><br></p>
€ 138,99
Levertijd ongeveer 8 werkdagen

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        Small Non-Coding RNAs