Protein Termini Part B

1. Proteome analysis of puromycin-labeled nascent polypeptides<br>Koshi Imami<br>2. HUNTER-DIA: An updated protocol for enrichment and mass spectrometry-based identification of protein N-termini<br>Pitter F. Huesgen<br>3. TERMINER - Bioinformatic detection and annotation of proteolytic protein termini in shotgun proteomics data<br>Oliver Schilling<br>4. Degronopedia: A practical guide to identifying and targeting protein degrons<br>Wojciech Pokrzywa<br>5. Quantitative Insights into Protein Turnover and Ubiquitination with HiBiT and NanoBRET<br>Wojciech Pokrzywa<br>6. Development of a flow cytometric method to evaluate the impact of N-terminal sequences on protein stability<br>Aditya M. Kunjapur<br>7. Generation and characterization of engineered N-degrons of the N-degron pathway using the ubiquitin-reference technique<br>Chang Hoon Ji<br>8. Characterization of the autophagic N-degron pathway and monitoring its chemical modulation for therapeutic development<br>Yong Tae Kwon<br>9. Characterization of the E3 ligase KCMF1 as a ZZ/N-recognin of the autophagic Arg/N-degron pathway<br>Chang Hoon Ji<br>10. Identification of Ac/N-degron-recognition domain within the MARCHF6 E3 ubiquitin ligase<br>Cheol-Sang Hwang<br>11. Affinity purification-mass spectrometry to identify nuclear protein interactions of N-terminal acetyltransferase NAA40<br>Antonis Kirmizis<br>12. TurboID technique for proximity labelling of interacting proteins<br>Greta Jarck<br>13. Chemical proteomic approaches to investigate S-prenylation<br>Edward W. Tate<br>14. Quantitative analysis of C-terminal prenylated protein levels using tandem mass tagging<br>Mark Distefano<br>15. Optimizing purification and FP-based binding assays for the E3 ligase FEM1C<br>Rong Huang