Apobec Enzymes

Section 1: Assays to characterize DNA or RNA editing activity<br>1. Fluorescent shift assay for APOBECs RNA editing<br>Xiaojiang S. Chen<br>2. Low Error Sequencing Methods to Tetect APOBEC-mediated RNA editing: Circular RNAseq and Safe-Sequencing System<br>Xiaojiang S. Chen<br>3. "Safe-Barcode" RNAseq assay for APOBECs RNA editing<br>Xiaojiang S. Chen<br>4. DT40 cell system to characterize somatic hypermutation<br>Silvestro Conticello<br>5. CH12 cell system to assay AID activity on class switch recombination<br>Silvestro Conticello<br>6. Purification of Enzymatically Active APOBEC Proteins from an Insect Cell Expression System<br>Linda Chelico<br>7. In vitro deamination assay to measure the activity and processivity of AID/APOBEC deaminases<br>Linda Chelico<br>8. Defining genome-wide mutagenic impact of APOBEC3 enzymes<br>Abby M. Green and Dávid Szüts<br>9. APOBEC-induced mutational assay in yeast<br>Steven Andrew Roberts<br><br>Section 2: Assays for APOBEC drug discovery<br>10. Biochemical assay for the identification of APOBECs inhibitors<br>Mani Larijani<br><br>Section 3: monitoring RNA or DNA editing<br>11. An In Vitro Cytidine Deaminase Assay to Monitor APOBEC activity on DNA<br>Rémi Buisson<br>12. Profiling rare C-to-U editing events via direct RNA sequencing<br>Ernesto Picardi and Adriano Fonzino<br>13. Global quantification of off-target activity by base editors<br>Erez Levanon and Michelle Eidelman<br><br>Section 4: Deaminases as tools for programmable RNA or DNA base editing<br>14. Programmable C to U RNA editing based on the APOBEC1 deaminase<br>Toshifumi Tsukahara<br><br>Section 5: Deaminases as tools for molecules tracking<br>15. Harnessing APOBEC editing for detecting RBP-RNA interactions<br>Gene W. Yeo and Tao Yu<br>16. Programmable C to U editing to track endogenous proteins<br>Tao Liu