1. TOM-TIM23 supercomplex formation<br>Peter Rehling, Naintara Jain and Ridhima Gomkale<br>2. Analysis of inner membrane lateral sorting at the preseqeunce translocase<br>Hyun Kim<br>3. Monitoring alpha-helical membrane protein insertion into the outer mitochondrial membrane of yeast cells<br>Thomas Becker<br>4. Monitoring alpha-helical membrane protein insertion into the outer mitochondrial membrane of human cells<br>Rebecca Voorhees<br>5. Mass spectrometry based analysis of mitochondrial protein translocation mutants and interactome studies<br>Bettina Warscheid<br>6. Analysis of protein trafficking between mitochondria and the endoplasmic reticulum by microscopy<br>Toshiya Endo<br>7. Monitoring retro-translocation of mitochondrial intermembrane space proteins<br>Piotr Bragoszewski and Magda Krakowczyk<br>8. Analysis of the evolutionary conservation of mitochondrial biogenesis pathways<br>Jeremy Grant Wideman and Kamawela P. Leka<br>9. Analysis of protein-protein interaction of the mitochondrial translocase at work by using technically effective BPA photo-crosslinking method<br>Takuya Shiota and Edward Germany<br>10. Analysis of mitochondrial protein translocation by disulfide bond formation and cysteine specific crosslinking<br>Nils Wiedemann and Laura F. Fielden<br>11. Analysis of the mitochondrial distribution of mitochondrial translocase complexes<br>Stefan Jacobs<br>12. ANALYSIS OF MITOCHONDRIAL TRANSLOCASES TOM AND TIM BY THE PATCH-CLAMPING TECHNIQUE<br>Maria Luisa Campo<br>13. Large-scale purification of mitochondrial protein complexes in yeast expression system for structural analyses<br>Yuhei 荒磯裕平(金沢大), Nanako Kobayashi, Yuta Imai, Shintaro Kita and Shiho Kawai<br>14. Generation of TIM chaperone substrate complexes<br>Paul Schanda, Undina Guillerm and Iva Sučec<br>15. Purification of functional recombinant human mitochondrial Hsp60<br>Abdussalam Azem and Celeste Weiss- Katz<br>16. Analysis of mitochondrial protein complex assembly by blue native PAGE in knock out cell lines<br>David A. Stroud, David Robinson and Liana Semcesen<br>17. Analysis of mitochondrial protein aggregation and disaggregation<br>Wolfgang Voos, Anna Wilkening, Robin Ostermann, Michael Bruderek, Witold Jaworek and Laura Ruland<br>18. Monitoring mitochondrial protein translocation regulation by phosphorylation<br>Chris Meisinger<br>19. Analysis of mitochondrial biogenesis regulation by oxidative stress<br>Naresh Babu V. Sepuri<br>20. Methods to analyze the mitochondrial unfolded protein response<br>Cole Haynes<br>21. Analysis of quality control pathways for the translocase of the outer mitochondrial membrane<br>Fabian den Brave and Lara Calvo Santos<br>22. Methods to analyze mitochondria associated degradation<br>Liza Pon<br>23. Monitoring and modulation of mitochondrial redox states<br>Jan Riemer<br>24. Seahorse assay for the mitochondrial respiration using Saccharomyces cerevisiae as a model system<br>Patrick D'silva<br>25. Screen for temperature-sensitive mutants of non-essential yeast genes<br>Nils Wiedemann, Nikolaus Pfanner and Iniyan Ganesan