Terpene Synthases
Specificaties
Gebonden, blz.
|
Engels
Elsevier Science |
e druk, 2024
ISBN13: 9780443238086
Rubricering
Onderdeel van serie
Methods in Enzymology
Verwachte levertijd ongeveer 9 werkdagen
Samenvatting
Terpene Synthases, Volume 700 in the Methods in Enzymology series, continues the legacy of this highly respected laboratory standard with its first dedicated collection on this important family of enzymes. Terpene synthases are a diverse set of enzymes that use exquisite mechanisms to form complex (poly)cyclic hydrocarbon skeletons. Chapters in this new volume include Structural analysis by X-ray crystallography and cryo-EM, Understanding mechanisms using stable isotopes, substrate analogs, or computational tools, Engineering fusion enzymes, Ancestral terpene cyclases, as well as the Sequence, structure, and function of non-canonical terpene synthases.
Specificaties
ISBN13:9780443238086
Taal:Engels
Bindwijze:Gebonden
Uitgever:Elsevier Science
Serie:Methods in Enzymology
Inhoudsopgave
1. Methods for the Expression, Purification, and Structural Analysis of a Bifunctional Class I Diterpene Synthase, Fusicoccadiene Synthase<br>2. Methods for the Expression, Purification, and Structural Analysis of a Bifunctional Class II Diterpene Synthase, Copalyl Diphosphate Synthase<br>3. X-ray Crystallography of Terpene Synthases<br>4. Isotopic Labellings for Mechanistic Studies<br>5. Understanding Mechanisms of Terpene Synthases Using Substrate Analogs<br>6. Docking Carbocations into Terpene Synthase Active Sites using Chemically Meaningful Constraints - The Terdockin Approach<br>7. A Practical Tutorial/Guide to EnzyDock<br>8. Bioinformatics of Terpene Synthases<br>9. Ancestral Terpene Cyclases: From Fundamental Science to Applications in Biosynthesis<br>10. Coral Terpene Synthases<br>11. Deciphering Magnesium Binding Site and Structure-Function Insights in Class II Sesquiterpene Cyclase<br>12. Functional Characterization of the UbiA-Family of Terpene Synthases<br>13. Functional Analysis of Transmembrane Terpene Cyclases Involved in Fungal Meroterpenoid Biosynthesis<br>14. Vanadium Haloperoxidases as Noncanonical Terpene Synthases<br>15. Structure, Function, and Engineering of Large Terpene Synthases<br>16. Coral Terpene Synthases (and perhaps some genomics and genome mining)<br>17. Engineering Terpene Synthases via Substrate Analogs<br>18. Engineering Terpene Synthase Fusions Enzymes