Biochemical Pathways and Environmental Responses in Plants: Part A

1. Structure, function, and engineering of plant polyketide synthases <br>Ikuro Abe<br>2. A sensitive LC-MS/MS assay for enzymatic characterization of methylthioalkylmalate　synthase involved in glucosinolate side-chain elongation<br>N Bisht<br>3. Assaying formate-tetrahydrofolate ligase with monoglutamylated and polyglutamylated substrates using a fluorescence-HPLC based assay <br>Sanja Roje<br>4. An Approach to Nearest Neighbor Analysis of Pigmented Protein Complexes by Using Chemical Crosslinking in Combination with Mass Spectrometry <br>Haijun Liu<br>5. Biochemical characterization of plant aromatic aminotransferases <br>Hiroshi Maeda<br>6. Functional Analysis of Phosphoethanolamine N-methyltransferase (PMT) in Plants and Parasites <br>Soon Goo Lee<br>7. A structure-guided computational screening approach for predicting plant enzyme-metabolite interactions <br>Cynthia Holland<br>8. Plant metacaspase: an example of microcrystal structure determination and analysis <br>Qun Liu<br>9. Biocatalytic system for comparative assessment of functional association of cytochrome P450 monooxygenases with their redox partners <br>Chang-Jun Liu<br>10. Dirigent Protein Family Function and Structure <br>Norman G. Lewis<br>11. Lignin/Lignan Biosynthesis Structure/Function <br>Norman G. Lewis<br>12. Discovery and characterization of BAHD acylsugar acyltransferases <br>Robert L. Last<br>13. Near-real time determination of BAHD acyl-coenzyme A transferase reaction rates and kinetic parameters using Ellman's reagent <br>Michael Sullivan<br>14. Quantification of N-terminal protein acetyltransferase activity in vitro and in planta <br>Markus Wirtz <br>15. Computational and biochemical methods to measure the activity of carboxysomes and protein organelles in vivo<br>Jeffrey Cameron<br>16. Analysis of Plant Alkaloid-Protein Interactions Using Differential Scanning Fluorimetry and Isothermal Titration Calorimetry <br>Peter Facchini<br>17. A metabolomics strategy for plant specialized metabolites using liquid chromatography-mass spectrometry <br>Kazuki Saito and Tetsuya Mori<br>18. Isoprene measurements to assess the methylerythritol pathway <br>Thomas D. Sharkey<br>19. Analysis of isoprenoid alcohol phosphates by liquid chromatorgraphy-mass spectrometry <br>Peter Dörmann and Katharina Gutbrod<br>20. Using targeted metabolomics to elucidate the indole auxin network in plants <br>Jerry D. Cohen and Adrian D. Hegeman<br>21. Metabolite fingerprinting: A powerful metabolomics approach for marker identification and functional gene annotation <br>Ivo Feussner<br>22. Ex vivo metabolomics, an hypothesis-free approach to identify native substrate(s) of orphan enzymes <br>Kirstin Feussner<br>23. Using stable isotopes and LC-MS for precursor of origin determination in plants <br>Clint Chapple <br>24. Measurement of flux through sulfate assimilation using [35S]sulfate <br>Stanislav Kopriva<br>25. Assessment of the activity of sulfate uptake and assimilation in plants <br>Rüdiger Hell<br>26. Comparison of TLC, HPLC, and direct infusion mass spectrometry methods for the identification and quantification of diacylglycerol molecular species <br>Philip Bates<br>27. Plant sphingolipid analysis <br>Jennifer E. Markham<br>28. Strategies to study the metabolic origins of plant specialized metabolites: the 1,4-naphthoquinones in Lithospermum erythrorhizon <br>Joshua R. Widhalm<br>29. Controlled environments for highly reproducible cannabis ‘omics’ studies <br>Bernd Markus Lange <br>30. Multi-platform approaches for the comprehensive analysis of aroma volatiles in cannabis <br>Bernd Markus Lange <br>31. Systems and strategies for plant protein expression <br>Thomas D. Niehaus <br>32. Programmable Chemical Actuator Control of Soluble and Membrane-Bound Enzymatic Catalysis <br>Katrina Cornish<br>33. CRISPR-Cas mediated genome engineering of cyanobacteria <br>Himadri B. Pakrasi <br>34. Plant Seed Proteome Analysis <br>Hari B. Krishnan<br>35. Proteomics and phosphoproteomics of C3 to CAM transition in the common ice plant <br>Sixue Chen<br>36. Identification of MPK4 interactome using TurboID proteomics <br>Sixue Chen<br>37. A Three-in-One Method for High Throughput Plant Multiomics <br>Sixue Chen <br>38. Assessing simultaneous reversible cysteine oxidation and global abundance in plant tissues <br>Leslie M. Hicks<br>39. Analysis of plant flooding response <br>Simon Gilroy<br>40. Imaging systemic calcium response and its molecular dissection using virus-induced gene silencing <br>Simon Gilroy<br>41. Studying the dynamics of transcriptional complex formation as a function of temperature using small angle x-ray scattering and in vitro assays <br>Chloe Zubieta<br>42. Isolation of novel chemical components and their target plant proteins in metal stress conditions <br>Ryoung Shin<br>43. Inhibition of insect Na+/K+　ATPases by plant-derived cardiac glycosides <br>Georg Petschenka Jander <br>44. High-throughput phenotyping and the use of computer vision to unravel novel phenotypes associated with plant nutrition <br>David Mendoza-Cozatl<br>45. Detection of protein persulfidation in plants by the dimedone switch method <br>Luis C. Romero and Cecilia Gotor<br>46. Using Hyper as a Molecular Probe to Visualize Hydrogen Peroxide in Living Plant Cells: An Updated Method <br>Luis Cárdenas<br>47. Genetically encoded sensors for monitoring the spatiotemporal dynamics of glucose and sucrose <br>Wolf B. Frommer and Nora Zoellner<br>48. Acetylene Reduction Assay for Nitrogenase Activity <br>Senthil Subramanian<br>49. Monitoring lipid-protein interactions　in planta　using　Förster resonance energy transfer <br>Xuemin (Sam) Wang<br>50. Modification of G-protein biochemistry and its effect on plant/environment interaction. <br>Sona Pandey<br>51. RNA-seq analysis of alternative pre-mRNA splicing mediated by photoreceptors in　Physcomitrium patens <br>Shih-Long Tu