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Advances in Protein Molecular and Structural Biology Methods

Specificaties
Paperback, blz. | Engels
Elsevier Science | e druk, 2022
ISBN13: 9780323902649
Rubricering
Elsevier Science e druk, 2022 9780323902649
€ 199,00
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Advances in Protein Molecular and Structural Biology Methods offers a complete overview of the latest tools and methods applicable to the study of proteins at the molecular and structural level. The book begins with sections exploring tools to optimize recombinant protein expression and biophysical techniques such as fluorescence spectroscopy, NMR, mass spectrometry, cryo-electron microscopy, and X-ray crystallography. It then moves towards computational approaches, considering structural bioinformatics, molecular dynamics simulations, and deep machine learning technologies. The book also covers methods applied to intrinsically disordered proteins (IDPs)followed by chapters on protein interaction networks, protein function, and protein design and engineering.

It provides researchers with an extensive toolkit of methods and techniques to draw from when conducting their own experimental work, taking them from foundational concepts to practical application.

Specificaties

ISBN13:9780323902649
Taal:Engels
Bindwijze:Paperback

Inhoudsopgave

<p>Section I: Techniques to optimize recombinant protein expression in bacteria, yeast, insect, and mammalian cells<br>1. Strategies to Improve the Expression and Solubility of Recombinant Proteins in Bacteria<br>Niharika Nag, Heena Khan, and Timir Tripathi<br>2. Advances in Heterologous Protein Expression Strategies in Yeast and Insect Systems<br>Meenakshi Singh, Smita Gupta, Arun Kumar Rawat, Sudhir Kumar Singh<br>3. Methods for Transient Expression and Purification of Monoclonal Antibodies in Mammalian Cells<br>Suchitra Kamle, Dawei Li, Chun Geun Lee, Jack A. Elias<br>4. Methods for Recombinant Production and Purification of Intrinsically Disordered Proteins<br>Steffen P. Graether</p> <p>Section 2: Biophysical techniques including, NMR, X-ray crystallography, cryo-EM, etc.<br>5. Methods to Determine the Oligomeric Structure of Proteins<br>Purna Bahadur Chetri, Heena Khan, and Timir Tripathi<br>6. Multimodal Methods to Study Protein Aggregation and Fibrillation<br>Maria Georgina Herrera, Marco Giampà, Nicolo Tonali and Veronica Isabel Dodero<br>7. Experimental Methods to Study the Thermodynamics of Protein-Protein Interactions<br>Santanu Sasidharan, Niharika Nag, Timir Tripathi, and Prakash Saudagar<br>8. Experimental Methods to Study the Kinetics of Protein-Protein Interactions<br>Abhay Narayan Singh, Kristijan Ramadan, Shalini Singh<br>9. Computational Techniques to Study Protein-Protein Interactions <br>Khattab Al-Khafaji, Tugba Taskin-Tok<br>10. Experimental Methods to Study Protein-Nucleic Acid Interactions<br>Roberto Giambruno, Jakob Rupert, and Elsa Zacco<br>11. Advanced Computational Tools for Quantitative Analysis of Protein-nucleic acid Interfaces<br>Sunandan Mukherjee and Chandran Nithin<br>12. Experimental Techniques to Study Protein Dynamics and Conformations<br>Akshita Gupta, Anamika Singh, Nabeel Ahmad, Tej P. Singh, Sujata Sharma, Pradeep Sharma<br>13. Computational Techniques to Study Protein Dynamics and Conformations <br>Laura Orellana, Anil Mhashal, and Agusti Emperador<br>14. Application of Circular Dichroism Spectroscopy in Studying Protein Folding, Stability, and Interaction<br>Md. Anzarul Haque, Punit Kaur, Asimul Islam, and Md. Imtaiyaz Hassan<br>15. Studying Protein Folding Dynamics using Single Molecule Fluorescence Methods<br>Narattam Mandal, Krishnananda Chattopadhyay, Achinta Sannigrahi<br>16. Advances in Liquid-state NMR Spectroscopy to Study the Structure, Function, and Dynamics of Biomacromolecules<br>Priyanka Aggarwal, Pooja Kumari and Neel Sarovar Bhavesh<br>17. In-Cell NMR Spectroscopy: A Tool for Cellular Structure Biology<br>Vijay Kumar<br>18. Current Trends in Membrane Protein Crystallography<br>Koomity V. Nageswar, Mansi Sharma, Dipak N. Patil, Santoshi Nayak, Anwesha Roy, Appu K. Singh<br>19. Advances in Sample Preparation and Data Processing for Single-particle Cryo-Electron Microscopy<br>Anshul Assaiya, Suparna Bhar and Janesh Kumar<br>20. Advanced Mass Spectrometry-Based Approaches for Molecular-Structural Biologists <br>Joanna Bons, Jacob Rose, Amy O’Broin, and Birgit Schilling<br>21. Developments, Advancements, and Contributions of Mass Spectrometry in Omics Technologies<br>Saravanan Kumar<br>22. Role of Structural Biology Methods in Drug Discovery<br>Fouzia Nasim and Insaf Ahmed Qureshi</p> <p>Section 3: Computational methods including MD simulation, coupling MD and deep machine learning, etc<br>23. Prediction, Validation, and Analysis of Protein Structures: A Beginner’s Guide<br>Santanu Sasidharan and Prakash Saudagar<br>24. Advances in Structure-Based Virtual Screening for Drug Discovery<br>Olujide O. Olubiyi, Suman Samantray and Alexander-Maurice Illig<br>25. Methods and Applications of Machine Learning in Structure-Based Drug Discovery<br>Madhumathi Sanjeevi, Prajna N Hebbar, Natarajan Aiswarya, Rashmi S., Chandrasekar Narayanan Rahul, Ajitha Mohan, Jeyaraman Jeyakanthan and Kanagaraj Sekar<br>26. Molecular Dynamics Simulations: Principles, Methods, and Applications in Protein Conformational Dynamics <br>Aditya K. Padhi, Matej Janezic, Kam Y. J. Zhang<br>27. Applications of Molecular Dynamics Simulations in Drug Discovery<br>Xubo Lin<br>28. Envisaging the Conformational Space of Proteins by Coupling Machine Learning and Molecular Dynamics<br>Murali Aarthy, Sanjeev Kumar Singh<br>29. Immunoinformatics and Reverse Vaccinology Methods to Design Peptide-based Vaccines<br>Vinita Sharma, Satyendra Singh, Tadi Sai Ratnakar, Vijay Kumar Prajapati</p> <p>Section 4: Tools and techniques to study IDPs<br>30. Computational Methods to Study Intrinsically Disordered Proteins<br>Prateek Kumar, Aparna Bhardwaj, Vladimir N. Uversky, Timir Tripathi, and Rajanish Giri<br>31. Experimental Methods to Study Intrinsically Disordered Proteins<br>Niharika Nag, Purna B Chetri, Vladimir N. Uversky, Rajanish Giri, and Timir Tripathi<br>32. Analysis of Structure and Dynamics of Intrinsically Disordered Regions in Proteins using Solution NMR Methods<br>Nikita V Saibo, Snigdha Maiti, Bidisha Acharya, and Soumya De<br>33. Methods to Study the Effect of Solution Variables on the Conformational Dynamics of IDPs<br>Hakan Alıcı, Orkun Hasekioglu, Vladimir N. Uversky, and Orkid Coskuner-Weber<br>34. Molecular Simulations to Study IDP-IDP Interactions and Their Complexes<br>Kota Kasahara</p> <p>Section 5: Protein interaction networks, signaling, engineering, and function, etc.<br>35. Exploring Large-Scale Protein Function using Systematic Mutant Analysis<br>Amrita Arpita Padhy, Subhashree Sahoo, Shivani Kumari, Varsha Kumari, Parul Mishra<br>36. Approaches and Methods to Study Cell Signaling: Linguistics of Cellular Communication<br>Siddharth Neog and Vishal Trivedi<br>37. Methods to Study Systems Biology of Signaling Networks: A Case Study of NSCLC<br>Nikhil H. Samarth and Shailza Singh<br>38. Advancements in the Analysis of Protein Post-Translational Modifications<br>Sandip Mukherjee, Ritesh Kumar, Arianne L. Theiss, and K. Venuprasad<br>39. Protein Engineering: Methods and Applications<br>Saurabh Bansal and Bishwajit Kundu<br>40. Designer 3D-DNA Nanodevices: Structures, Functions and Cellular Applications<br>Kahkashan Bansal, Anjali Rajwar, and Dhiraj Bhatia</p>
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        Advances in Protein Molecular and Structural Biology Methods