Monitoring Vesicular Trafficking in Cellular Responses to Stress - Part B

Specificaties
Gebonden, blz. | Engels
Elsevier Science | e druk, 2021
ISBN13: 9780128244876
Rubricering
Elsevier Science e druk, 2021 9780128244876
Onderdeel van serie Methods in Cell Biology
Verwachte levertijd ongeveer 8 werkdagen

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Monitoring Vesicular Trafficking in Cellular Responses to Stress - Part B, Volume 165 in the Methods in Cell Biology series, highlights new advances in the field, with this new release presenting coverage of different topics, including A novel tool for detecting lysosomal membrane permeabilization by high-throughput fluorescence microscopy, Exploring selective autophagy in Drosophila, Assessment of EGFP-Q74 degradation for the measurement of autophagic flux, Multimodal assessment of autophagy in mammalian cells with a novel, LC3-based tandem reporter, Multiplex quantification of autophagic flux by imaging flow cytometry, Monitoring autophagic flux in Caenorhabditis elegans using p62/SQST-1 reporters, High throughput screening for autophagy, and much more.

Specificaties

ISBN13:9780128244876
Taal:Engels
Bindwijze:Gebonden

Inhoudsopgave

<p>1. A novel tool for detecting lysosomal membrane permeabilization by high-throughput fluorescence microscopy<br>Karla Alvarez-Valadez, Allan Sauvat, Helene Fohrer-Ting, Christophe Klein, Oliver Kepp, Guido Kroemer, Mojgan Djavaheri-Mergny<br>2. Exploring selective autophagy in Drosophila<br>Stavroula Petridi, Anne-Claire Jacomin, Zambarlal Bhujabal, Terje Johansen and Ioannis P. Nezis<br>3. Assessment of EGFP-Q74 degradation for the measurement of autophagic fluxYan Wang, Jiao Meng, Qi Wu, Guo Chen, Wei Xie, Zhen Zhang, Guido Kroemer and Oliver Kepp<br>4. Multimodal assessment of autophagy in mammalian cells with a novel, LC3-based tandem reporter<br>Dan F. Lazar, Amani A. Gillette, Samantha R. Lewis, and Braeden L. Butler<br>5. Multiplex quantification of autophagic flux by imaging flow cytometry<br>Léa Montegut, Hui Chen, Gerasimos Anagnostopoulos, Sabrina Spaggiari, Oliver Kepp, Maria Chiara Maiuri, Guido Kroemer, and Isabelle Martins<br>6. Monitoring autophagic flux in Caenorhabditis elegans using p62/SQST-1 reporters<br>Christina Ploumi, Aggeliki Sotiriou and Nektarios Tavernarakis<br>7. High throughput screening for autophagy<br>Sabrina Forveille, Marion Leduc, Allan Sauvat, Giulia Cerrato, Guido Kroemer and Oliver Kepp<br>8. Determination of mitophagy by electron microscope<br>Zhiyu Li, Qi Wu, Le Liu, Si Sun, Shengrong Sun,Zhong Wang, Juanjuan Li<br>9. Quantification of intracellular ACBP/DBI levels<br>Valentina Sica, Isabelle Martins, Federico Pietrocola and José Manuel Bravo-San Pedro<br>10. Immunoblot-based assays for assessing autophagy in the turquoise killifish Nothobranchius furzeri<br>Gerasimos Anagnostopoulos, Leìa Montegut, Hui Chen, Vincent Carbonnier, Isabelle Martins, Khady Mangane, Abdelkrim Mannioui, Alex Bois, Elisa Elena Baracco, Maria Chiara Maiuri, and Guido Kroemer<br>11. Monitoring autophagy using super-resolution structured illumination and direct stochastic optical reconstruction microscopy<br>Dumisile Lumkwana, Lize Engelbrecht and Ben Loos<br>12. Monitoring selective autophagy of mitochondria using super-resolution microscopy<br>Ziyue Li and Nicholas T. Ktistakis<br>13. Monitoring autophagy-dependent ferroptosis<br>Jingbo Li, Rui Kang, and Daolin Tang<br>14. Quantitative analysis of autophagy-related protein LC3B by quantum-dot-based molecular imaging<br>Xin Yu, Juan Wu, Qi Wu, Shengrong Sun<br>15. Isolation of microRNA from exosomes<br>Sheila Spada<br>16. Simultaneous determination of intraluminal lysosomal calcium and pH by dextran-conjugated fluorescence dyes<br>Philippe Pihán, Paula Nunes-Hasler, Nicolas Demaurex and Claudio Hetz</p>

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        Monitoring Vesicular Trafficking in Cellular Responses to Stress - Part B