1. An introduction to advanced technologies in synthetic biology<br>Vijai Singh, Gargi Bhattacharjee, Nisarg Gohil, Rupesh Maurya, Navya L. Lam and Khalid J. Alzahrani<br>2. Design and characterization of synthetic promoters<br>Boris Kirov and Slavil Peykov<br>3. Recent development in DNA synthesis technology<br>Indra Mani<br>4. Advances, challenges, and opportunities in DNA sequencing technology<br>Francisco J. Enguita and Ana Lucia Leitao<br>5. Recent developments in genome design and assembly tools<br>Carlos Barreiro and Carlos Garcıa-Estrada<br>6. Design, building, and challenges in synthetic genomics<br>Mansoor A. Siddiqui, Ashima Sharma and Syed Shams Yazdani<br>7. Synthetic minimal cells and their applications<br>Ali Yazdizadeh Kharrazi, Amir Zare, Nils Chapin, Seyedkhashayar Ghavami and Amir Pandi<br>8. Small RNA-based systems for sensing and therapeutic applications<br>Robert Penchovsky, Georgi Y. Miloshev, Nikolet Pavlova, Katya B. Popova, Aikaterini Valsamatzi-Panagiotou, Lozena A. Otcheva and Martina Traykovska<br>9. High-throughput navigation of the sequence space<br>Alaksh Choudhury<br>10. Advances in protein engineering and its application in synthetic biology<br>Rongming Liu, Liya Liang, Maria Priscila Lacerda, Emily F. Freed and Carrie A. Eckert<br>11. Computational tools for design of synthetic genetic circuits<br>Archit Devarajan, Dhwani Gupta, Kushika Mitra, Shalini S. Deb and Shamlan M.S. Reshamwala<br>12. Computational tools for synthetic gene optimization<br>Numaan Cheema, Georgios Papamichail and Dimitris Papamichail<br>13. De novo design and synthesis of biomolecules<br>Subha Sankar Paul, Debarun Dhali, Yazen Yaseen, Upasana Basu, Shilpa Pal, Nihal Mohammed and Heykel Trabelsi<br>14. A retrobiosynthetic approach for production, conversion, sensing, dynamic regulation and degradation of molecules<br>Pablo Carbonell<br>15. Advances in engineering of bacteriophages for therapeutic applications<br>Christian Møller-Olsen, Gurneet K. Dhanoa, Tamas Feher and Antonia P. Sagona<br>16. Design of synthetic biology for the detection of microorganisms<br>Dong-Dong Yang, Jake Flood, Rongming Liu and Susu He<br>17. Design of synthetic biological devices for detection and targeting human diseases<br>Nedim Haciosmanoglu, Sıla Kose, Julian Ostaku, Ilkay Cisil Koksaldi, Behide Saltepe and Urartu Ozgur Safak Seker<br>18. Engineering cell-based therapies<br>Juan Luis Blazquez-Roman, Mara Pisani and Velia Siciliano<br>19. Development of next-generation diagnostic tools using synthetic biology<br>Elizabeth J. Vojvoda, Logan R. Burrington and Javin P. Oza<br>20. Advances and application of CRISPR-Cas systems<br>Rongming Liu, Liya Liang, Margaret Habib, Emily F. Freed and Carrie A. Eckert<br>21. Synthetic biology for smart drug biosynthesis and delivery<br>Srinivasan Ganesh Kumar, Ashok Ganesan and Shashi Kumar<br>22. Design and applications of self-assembled soft living materials using synthetic biology<br>Gokce Ozkul, Merve Yavuz, Nedim Hacıosmanoglu, Busra Merve Kırpat and Urartu Ozgur Safak Seker<br>23. Synthetic gene circuits for higher-order information processing<br>Kathakali Sarkar and Sangram Bagh<br>24. Cell-free synthetic biology as an emerging biotechnology<br>Seok Hoon Hong and Ana Serratosa Fernandez-Baca<br>25. Recent development and applications of xeno nucleic acids<br>Rupesh Maurya, Nisarg Gohil, Gargi Bhattacharjee, Navya L. Lam, Khalid J. Alzahrani and Vijai Singh<br>26. Developments in the use of microfluidics in synthetic biology<br>Laila A. Damiati, Safa A. Damiati and Samar Damiati<br>27. Implications of synthetic biology research and development: a structured ethical analysis<br>Kevin Smith<br>