Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Chemical, Optical and Bioorthogonal Methods

<ol> <ol> <li>Enzyme- or light-triggered cyclopropenes for bioorthogonal ligation</li> <p>Ting Jiang and Scott T. Laughlin</p> <p> <li>Analysis of metabolically labeled inositol phosphate messengers by NMR</li> <p>Robert Puschmann, Robert K. Harmel and Dorothea Fiedler</p> <p> <li>Photo-releasable derivatives of inositol pyrophosphates</li> <p>Sebastian Hauke, Tamara Bittner, Henning J. Jessen and Carsten Schultz</p> <p> <li>IMPACT: Imaging phospholipase D activity with clickable alcohols via transphosphatidylation</li> <p>Timothy W. Bumpus, Dongjun Liang and Jeremy M. Baskin</p> <p> <li>Fluorescent bioorthogonal labeling of class B GPCRs in live cells</li> <p>Srikanth Kumar Gangam and Qing Lin</p> <p> <li>Near-infrared photoactivatable nitric oxide donors with photoacoustic readout </li> <p>Effie Y. Zhou, Hailey J. Knox, Christopher J. Reinhardt, Gina Partipilo and Jefferson Chan</p> <p> <li>H<SUB>2</SUB>S donors with optical responses</li> <p>Michael D. Pluth, Yu Zhao and Matthew M. Cerda</p> <p> <li>Visualization of oxidative stress-induced carbonylation in live mammalian cells</li> <p>Kamalika Mukherjee, Tak Ian Chio and Susan L. Bane</p> <p> <li>Receptor-based fluorescent sensors constructed from ribonucleopeptide</li> <p>Skun Nakano, Hiroaki Konishi and Takashi Morii</p> <p> <li>Conformationally restrained pentamethine cyanines and use in reductive single molecule localization microscopy</li> <p>Siddharth S. Matikonda, Ralph Götz, Ryan McLaughlin, Markus Sauer and Martin J. Schnermann</p> <p> <li>A near-infrared light-mediated cleavable linker strategy using the heptamethine cyanine chromophore</li> <p>Michael P. Luciano, Saghar Nourian, Alexander P. Gorka, Roger R. Nani, Tadanobu Nagaya, Hisataka Kobayashi and Martin J. Schnermann</p> <p> <li>Quantitative measurement of cytosolic penetration using the chloroalkane penetration assay</li> <p>Kirsten Deprey and Joshua A. Kritzer</p> <p> <li>Cell-penetrating and mitochondrion-targeting molecules</li> <p>George Appiah Kubi and Dehua Pei</p> <p> <li>A photocaged DNA nanocapsule for delivery and manipulation in cells</li> <p>Yihong Feng, Takeshi Tohgasaki, Yasuyuki Shitomi, Hiroshi Sugiyama and Masayuki Endo</p> <p> <li>A multicolor riboswitch-based platform for imaging of RNA in live mammalian cells</li> <p>Esther Braselmann and Amy E. Palmer</p> <p> <li>Enzymatic covalent labeling of RNA with RNA transglycosylation at guanosine (RNA-TAG)</li> <p>Kayla N. Busby and Neal K. Devaraj</p> <p> <li>Assaying RNA solvent accessibility in living cells with LASER</li> <p>Chao Feng and Robert C. Spitale</p> <p> <li>Split T7 RNA polymerase biosensors to study multiprotein interaction dynamics</li> <p>Jeffrey A. Dewey and Bryan C. Dickinson</p> <p> <li>Cross-linking cellular nucleic acids via a target-directing double click reagent</li> <p>Masayuki Tera and Nathan W. Luedtke</p> <p> <li>Click chemistry-based amplification and detection of endogenous RNA and DNA molecules in situ using clampFISH probes</li> <p>Sepideh Tavakoli, Yifang Liu, Jacob Potts and Sara H. Rouhanifard</p> <p> <li>Quantification of protein delivery in live cells using fluorescence correlation spectroscopy </li></ol></ol> <p>Susan L. Knox, Angela Steinauer, Garrett Alpha-Cobb, Adam Trexler, Elizabeth Rhoades and Alanna Schepartz</p>