Tumor Immunology and Immunotherapy – Molecular Methods

<p>1. Detection of circulating tumor DNA (ctDNA) by digital droplet polymerase chain reaction (dd-PCR) in liquid biopsies</p> <p>Sara Valpione and Luca Campana</p> <p>2. Detection and quantification of cytosolic DNA</p> <p>Sheila Spada, Takahiro Yamazaki and Claire Vanpouille-Box</p> <p>3. Methods to detect endogenous dsRNA induction and recognition</p> <p>Ilias Ettayebi, Helen Loo Yau and Daniel D. De Carvalho</p> <p>4. Quantification of eIF2alpha phosphorylation during immunogenic cell death</p> <p>Lucillia Bezu, Alejandra Wu Chuang, Juliette Humeau, Guido Kroemer and Oliver Kepp</p> <p>5. Assessment of annexin A1 release during immunogenic cell death</p> <p>Elisa Elena Baracco, Adriana Petrazzuolo and Guido Kroemer</p> <p>6. Luciferase-assisted detection of extracellular ATP and ATP metabolites during immunogenic death of cancer cells</p> <p>George R. Dubyak</p> <p>7. Quinacrine-mediated detection of intracellular ATP</p> <p>Sabrina Forveille, Juliette Humeau, Allan Sauvat, Lucillia Bezu, Guido Kroemer and Oliver Kepp</p> <p>8. Structure, function and techniques of investigation of the P2X7 receptor (P2X7R) in mammalian cells</p> <p>Francesco Di Virgilio, Lin-Hua Jiang, Sèbastien Roger, Simonetta Falzoni, Alba Clara Sarti, Valentina Vultaggio-Poma, Paola Chiozzi and Elena Adinolfi</p> <p>9. LEGENDplex™: Bead-assisted multiplex cytokine profiling by flow cytometry</p> <p>Jason S. Lehmann, Priyanka Rughwani, Melissa Kolenovic, Shaoquan Ji and Binggang Sun</p> <p>10. Methods for measuring HMGB1 release during immunogenic cell death</p> <p>Liwei Zhao, Peng Liu, Oliver Kepp and Guido Kroemer</p> <p>11. Different measures of HMGB1 location in cancer immunology</p> <p>Carolina Mendonça Gorgulho, Pranav Murthy, Lance Liotta, Virginia Espina and Michael T. Lotze</p> <p>12. Reliable detection of indoleamine 2,3 dioxygenase-1 in murine cells and tissues</p> <p>Sunil Thomas, Lisa Laury-Kleintop and George C. Prendergast</p> <p>13. Quantification of IDO1 enzyme activity in normal and malignant tissues</p> <p>Lijie Zhai, Erik Ladomersky, April Bell, Corey Dussold, Krislyn Cardoza, Jun Qian, Kristen L. Lauing and Derek A. Wainwright</p> <p>14. Enzyme activity of circulating CD73 in human serum</p> <p>Silvana Morello, Roberta Turiello, Gabriele Madonna, Aldo Pinto, Paolo A. Ascierto and<br>Mariaelena Capone</p> <p>15. Measurement of CD73 enzymatic activity using luminescence-based and colorimetric assays</p> <p>Bertrand Allard, Isabelle Cousineau, Kathleen Spring and John Stagg</p> <p>16. Distinguishing perforin-mediated lysis and granzyme-dependent apoptosis</p> <p>Jesse A. Rudd-Schmidt, Joseph A. Trapani and Ilia Voskoboinik</p> <p>17. Elucidating the role of an immunomodulatory protein in cancer: From protein expression to functional characterization</p> <p>Leepakshi Khurana, Mei ElGindi, Pathricia V. Tilstam and Georgios Pantouris</p> <p>18. In vitro assay for the development of small molecule inhibitors targeting PD-1/PD-L1</p> <p>Wenjie Zhai, Xiuman Zhou, Jiangfeng Du and Yanfeng Gao</p> <p>19. Crystallographic approaches to study the interaction modes of PD-1- and CTLA-4-blocking antibodies</p> <p>Norimichi Nomura, Yayoi Nomura, Yumi Sato and So Iwata</p> <p>20. High-throughput discovery of cancer-targeting TCRs</p> <p>Lukas Bunse, Edward W. Green and Michael Platten</p> <p>21. TCR validation toward gene therapy for cancer</p> <p>Edward W. Green, Lukas Bunse, Matthias Bozza, Khwab Sanghvi and Michael Platten</p> <p>22. Analysis of TCR β CDR3 sequencing data for tracking anti-tumor immunity</p> <p>Jiajia Zhang, Zhicheng Ji and Kellie N. Smith</p> <p>23. Quantitative analysis of the T cell receptor repertoire</p> <p>Imran Uddin, Annemarie Woolston, Thomas Peacock, Kroopa Joshi, Mazlina Ismail, Tahel Ronel, Connor Husovsky and Benny Chain</p> <p>24. CRATES: A one-step assembly method for Class 2 CRISPR arrays</p> <p>Chunyu Liao, Rebecca A. Slotkowski and Chase L. Beisel</p>