Post-translational Modifications That Modulate Enzyme Activity

<p>1. Crosstalk between cellular metabolism and histone acetylation<br>Sophie Trefely, Mary T. Doan and Nathaniel W. Snyder<br>2. Purification and enzymatic assay of class I histone deacetylase enzymes<br>Mark K. Adams, Charles A.S. Banks, Sayem Miah, Maxime Killer and Michael P. Washburn<br>3. Multiplexed quantitative phosphoproteomics of cell line and tissue samples<br>Johannes Kreuzer, Amanda Edwards and Wilhelm Haas<br>4. Differentiation of peptide isomers and epimers by radical-directed dissociation<br>Tyler R. Lambeth and Ryan R. Julian<br>5. Biochemical analysis of protein arginylation<br>Junling Wang, John R. Yates and Anna Kashina<br>6. Site-specific determination of lysine acetylation stoichiometries on the proteome-scale<br>Yue Chen and Yunan Li<br>7. RNA modifications and the link to human disease<br>Amber Yanas and Kathy Fange Liu<br>8. Isolation and characterization of glycosylated neuropeptides<br>Yang Liu, Qinjingwen Cao and Lingjun Li<br>9. Utilizing intein trans-splicing for in vivo generation of site-specifically modified proteins<br>Igor Maksimovic, Devin Ray, Qingfei Zheng and Yael David<br>10. Systematic and site-specific analysis of N-glycoproteins on the cell surface by integrating bioorthogonal chemistry and MS-based proteomics<br>Fangxu Sun and Ronghu Wu<br>11. Silencing glycosaminoglycan functions in mouse embryonic stem cells with small molecule antagonists<br>Sourav Chatterjee, Tesia N. Stephenson, Austen L. Michalak, Kamil Godula and Mia L. Huang<br>12. Biochemical and structural analysis of N-terminal acetyltransferases<br>Leah Gottlieb and Ronen Marmorstein<br>13. Site-specific analysis of the Asp- and Glu-ADP-ribosylated proteome by quantitative mass spectrometry<br>Peng Li, Yuanli Zhen and Yonghao Yu<br>14. Analysis of the topology of ubiquitin chains<br>Lucia Geis-Asteggiante, Amanda E. Lee and Catherine Fenselau<br>15. Analysis of cardiac troponin proteoforms by top-down mass spectrometry<br>Timothy N. Tiambeng, Trisha Tucholski, Zhijie Wu, Yanlong Zhu, Stanford D. Mitchell, David S. Roberts, Yutong Jin and Ying Ge<br>16. Assays for tyrosine phosphorylation in human cells<br>Monica Kruk, Naomi Widstrom, Sampreeti Jena, Nicole L. Wolter, John F. Blankenhorn, Ibrahim Abdalla, Tzu-Yi Yang and Laurie L. Parker<br>17. Metabolomics analysis of lipid metabolizing enzyme activity<br>Timothy B. Ware, Myungsun Shin and Ku-Lung Hsu<br>18. Simplified high yield TAILS terminomics using a new HPG-ALD 800K-2000 polymer with precipitation<br>Nestor Solis, Anilkumar Parambath, Srinivas Abbina, Jayachandran Kizhakkedathu and Christopher M. Overall<br>19. Methods for the expression, purification, and crystallization of histone deacetylase 6–inhibitor complexes<br>Jeremy D. Osko and David W. Christianson<br>20. Quantitative analysis of global protein lysine methylation by mass spectrometry<br>Peder J. Lund, Stephanie M. Lehman and Benjamin A. Garcia<br>21. The roles of S-nitrosylation and S-glutathionylation in Alzheimer’s disease<br>Ryan R. Dyer, Katarena I. Ford and Renã A. S. Robinson<br>22. Expression of authentic post-translationally modified proteins in organisms with expanded genetic codes<br>Kyle Mohler and Jesse Rinehart<br>23. Preparation of a new construct of human histone deacetylase 8 for the crystallization of enzyme-inhibitor complexes<br>Nicholas J. Porter and David W. Christianson<br>24. Methods for characterizing protein acetylation during viral infection<br>Laura A. Murray, Ashton N. Combs, Pranav Rekapalli and Ileana M. Cristea</p>