DNA Sensors and Inflammasomes

<p>1. Intercellular communication in the innate immune system through the cGAS-STING pathway<br>Ramya Nandakumar, Samuel Joseph Windross and Søren R. Paludan<br>2. Analysis of human cGAS activity and structure<br>Wen Zhou, Aaron T. Whiteley and Philip J. Kranzusch<br>3. Chemical synthesis, purification, and characterization of 3′-5′-linked canonical cyclic dinucleotides (CDNs)<br>Changhao Wang, Min Hao, Qianqian Qi, Yashao Chen and Jörg S. Hartig<br>4. An SPR-based analysis of cGAS substrate K<SUB>D</SUB> and steady-state K<SUB>M</SUB> values<br>Erik C. Ralph and Justin Hall<br>5. A pyrophosphatase-coupled assay to monitor the NTase activity of cGAS<br>Richard Hooy and Jungsan Sohn<br>6. Tracking the polymerization of DNA sensors and inflammasomes using FRET<br>Zachary Mazanek and Jungsan Sohn<br>7. Monitoring gasdermin pore formation in vitro<br>Shiyu Xia, Jianbin Ruan and Hao Wu<br>8. Measuring TREX1 and TREX2 exonuclease activities<br>Wayne O. Hemphill and Fred W. Perrino<br>9. Kinetics of DNA: Protein association and dissociation by stopped-flow spectroscopy<br>Johannes Rudolph and Karolin Luger<br>10. Characterization of DNA bound cyclic GMP-AMP synthase using atomic force microscopy imaging<br>Alexander Lushnikov, Richard Hooy, Jungsan Sohn and Alexey Krasnoslobodtsev<br>11. Preparation of filamentous proteins for electron microscopy visualization and reconstruction<br>Mariusz Matyszewski and Jungsan Sohn<br>12. Cryo-EM studies of NAIP–NLRC4 inflammasomes<br>Nicole Haloupek, Patricia Grob, Jeannette Tenthorey, Russell E. Vance and Eva Nogales<br>13. An integrative protocol for the structure determination of the mouse ASC-PYD filament<br>Adam Mazur, Petr Broz and Sebastian Hiller<br>14. Protein interactions of the inflammasome adapter ASC by solution NMR<br>Pedro Diaz-Parga and Eva de Alba<br>15. Tracking DNA sensor and inflammasome complexes in cells using confocal microscopy<br>Brendan Antiochos<br>16. Assessment of inflammasome and type I IFN responses to DNA viruses and DNA PAMPS<br>Guido Massaccesi and Michael A. Chattergoon<br>17. Biochemical and microscopic analysis of inflammasome complex formation<br>Patrycja Swacha, Nelson Ong’ondo Gekara and Saskia F. Erttmann<br>18. Comet and micronucleus assays for analyzing DNA damage and genome integrity<br>Hui Jiang and Nelson Ong'ondo Gekara<br>19. Workflows and considerations for investigating protein interactions of viral DNA sensors<br>Timothy R. Howard, Bokai Song and Ileana M. Cristea<br>20. TUBE and UbiCRest assays for elucidating polyubiquitin modifications in protein complexes<br>Swarupa Panda and Nelson Ong'ondo Gekara</p>