Chemical and Synthetic Biology Approaches to Understand Cellular Functions - Part A

<p>1. Targeted m<SUP>6</SUP>A reader proteins to study the epitranscriptome<br>Simone Rauch and Bryan C. Dickinson<br>2. Synthetic gene expression circuits regulating sexual reproduction<br>Nobuo Fukuda and Shinya Honda<br>3. Nucleotide resolution sequencing of N4-acetylcytidine in RNA<br>Justin M. Thomas, Keri M. Bryson and Jordan L. Meier<br>4. A guide for drug inducible genome editing with HIT systems<br>Chen Zhao, Shixian Wei and Yu Wang<br>5. A guide for drug inducible transcriptional activation with HIT systems<br>Chen Zhao, Shixian Wei and Yu Wang<br>6. Direct cloning and heterologous expression of natural product biosynthetic gene clusters by transformation-associated recombination<br>Jia Jia Zhang, Kazuya Yamanaka, Xiaoyu Tang and Bradley S. Moore<br>7. Salt-sensitive intein for large-scale polypeptide production<br>Yi-Zong Lee and Shih-Che Sue<br>8. Methods for the recombinant expression of active tyrosine kinase domains: Guidelines and pitfalls<br>M. Escarlet Díaz Galicia, Abdullah Aldehaiman, SeungBeom Hong, Stefan T. Arold and Raik Grünberg<br>9. Design, cloning and characterization of transcription factor-based inducible gene expression systems<br>Erik K.R. Hanko, Nigel P. Minton and Naglis Malys<br>10. Design, construction, and validation of optogenetic proteins<br>Colin P. O'Banion, Anwesha Goswami and David S. Lawrence<br>11. Overcoming component limitations in synthetic biology through transposon-mediated protein engineering<br>Joshua T. Atkinson, Bingyan Wu, Laura Segatori and Jonathan J. Silberg<br>12. Chemical biology of glycoproteins: From chemical synthesis to biological impact<br>Yaohao Li, Amy H. Tran, Samuel J. Danishefsky and Zhongping Tan<br>13. In bulla functional channel expression systems that mimic bacterial synthetic membranes<br>Masayuki Iwamoto and Shigetoshi Oiki<br>14. A mass spectrometry-based isotope-coded mass tag method to map thiol accessibility in biological systems<br>John E. Gadbery and Nicole S. Sampson<br>15. Quick-soaking of crystals reveals unprecedented insights into the catalytic mechanism of glycosyltransferases<br>David Albesa-Jové, Javier O. Cifuente, Beatriz Trastoy and Marcelo E. Guerin<br>16. Cysteine-ethylation of tissue-extracted membrane proteins as a tool to detect conformational states by solid-state NMR spectroscopy<br>Daniel K. Weber, Taysir Bader, Erik K. Larsen, Songlin Wang, Tata Gopinath, Mark Distefano and Gianluigi Veglia<br>17. Improved sensitivity and resolution of in-cell NMR spectra<br>David S. Burz, Leonard Breindel and Alexander Shekhtman<br>18. High-throughput methods in aptamer discovery and analysis<br>Kyle H. Cole and Andrej Lupták<br>19. Drop-in-well chamber for droplet interface bilayer with built-in electrodes<br>Kazuhiro Urakubo, Masayuki Iwamoto and Shigetoshi Oiki</p>