Intrinsically Disordered Proteins

<p>1. Phase Separation of Intrinsically Disordered Proteins<br>Ammon E. Posey, Alex S. Holehouse and Rohit V. Pappu<br>2. Methods and Strategies to Quantify Phase Separation of Disordered Proteins <br>Alfredo Vidal Ceballos, Charles J. McDonald and Shana Elbaum-Garfinkle<br>3. Visualization and Quantitation of Phase-Separated Droplet Formation by Human HP1α<br>Madeline M. Keenen, Adam G. Larson and Geeta J. Narlikar<br>4. Probing RNA Structure in Liquid–Liquid Phase Separation Using SHAPE-MaP<br>Erin M. Langdon and Amy S. Gladfelter<br>5. The Use of <SUP>13</SUP>C Direct-Detect NMR to Characterize Flexible and Disordered Proteins<br>Erik C. Cook, Grace A. Usher and Scott A. Showalter<br>6. Using NMR Chemical Shifts to Determine Residue-Specific Secondary Structure Populations for Intrinsically Disordered Proteins<br>Wade M. Borcherds and Gary W. Daughdrill<br>7. Experimental Characterization of Fuzzy Protein Assemblies: Interactions of Paramyxoviral N<SUB>TAIL</SUB> Domains With Their Functional Partners<br>Francesca Troilo, Christophe Bignon, Stefano Gianni, Monika Fuxreiter and Sonia Longhi<br>8. Characterization of Dynamic IDP Complexes by NMR Spectroscopy<br>Andreas Prestel, Katrine Bugge, Lasse Staby, Ruth Hendus-Altenburger and Birthe B. Kragelund<br>9. Spectroscopic Characterization of Structure–Function Relationships in the Intrinsically Disordered Protein Complexin<br>David Snead and David Eliezer<br>10. Distances, Distance Distributions, and Ensembles of Unfolded and Intrinsically Disordered Proteins From Single-Molecule FRET<br>Erik D. Holmstrom, Andrea Holla, Wenwei Zheng, Daniel Nettles, Robert B. Best and Benjamin Schuler<br>11. Probing Differential Binding Mechanisms of Phenylalanine-Glycine-Rich Nucleoporins by Single-Molecule FRET<br>Piau Siong Tan and Edward A. Lemke<br>12. Fluorescence Depolarization Kinetics to Study the Conformational Preference, Structural Plasticity, Binding, and Assembly of Intrinsically Disordered Proteins<br>Anupa Majumdar and Samrat Mukhopadhyay<br>13. Building, Characterization, and Applications of Cuvette-FCS in Denaturant-Induced Expansion of Globular and Disordered Proteins<br>Timir Baran Sil, Bankanidhi Sahoo and Kanchan Garai<br>14. Stopped-Flow Kinetic Techniques for Studying Binding Reactions of Intrinsically Disordered Proteins<br>Michael D. Crabtree and Sarah L. Shammas<br>15. The Use of Mass Spectrometry to Examine IDPs: Unique Insights and Caveats<br>Dale Stuchfield, Aidan France, Lukasz G. Migas, Anja Thalhammer, Anne Bremer, Bruno Bellina and Perdita E. Barran<br>16. Characterizing Time-of-Day Conformational Changes in the Intrinsically Disordered Proteins of the Circadian Clock<br>Jacqueline F. Pelham, Alexander E. Mosier and Jennifer M. Hurley<br>17. Assessing Allostery in Intrinsically Disordered Proteins With Ensemble Allosteric Model<br>Jing Li and Vincent J. Hilser<br>18. A Tethered Vesicle Assay for High-Throughput Quantification of Membrane Fission<br>Wilton T. Snead and Jeanne C. Stachowiak<br>19. Generating Novel Materials Using the Intrinsically Disordered Protein Ubx<br>Gabriela Geraldo Mendes, Rebecca M. Booth, Donna L. Pattison, Adrian J. Alvarez and Sarah E. Bondos<br>20. Challenges in the Structural–Functional Characterization of Multidomain, Partially Disordered Proteins CBP and p300: Preparing Native Proteins and Developing Nanobody Tools<br>Angela Bekesi, Sara Abdellaoui, Natalie Holroyd, Wouter Van Delm, Els Pardon, Jarne Pauwels, Kris Gevaert, Jan Steyaert, Stefaan Derveaux, Antoni Borysik and Peter Tompa<br>21. Characterization of the Binding of Small Molecules to Intrinsically Disordered Proteins<br>Veselin S. Dobrev, Lisette M. Fred, Kaitlyn P. Gerhart and Steven J. Metallo<br>22. Targeting the Intrinsically Disordered Proteome Using Small Molecule Ligands<br>Sławomir Wójcik, Melissa Birol, Elizabeth Rhoades, Andrew D. Miranker and Zachary A. Levine</p>