<p>Section 1: Methods for Studying Microorganisms in Space<br>1. Microbiology of the Built Environment in Spacecraft Used for Human Flight<br>Jiseon Yang, Starla G. Thornhill, Jennifer Barrila, Cheryl A. Nickerson, Charlie M. Ott and Robert J.C. McLean<br>2. The Study of Microbial Survival in Extraterrestrial Environments Using Low Earth Orbit and Ground-Based Experiments<br>Karen Olsson-Francis, Nisha K. Ramkissoon, Alex B. Price, David J. Slade, Michael Macey and Victoria K. Pearson<br>3. Persistence of Fungi in Atypical, Closed Environments: Cultivation to Omics<br>Adriana Blachowicz, Kasthuri Venkateswaran and Clay C.C. Wang</p> <p>Section 2: Molecular Methods<br>4. Molecular Methods for Studying Microorganisms From Atypical Environments<br>Hirak Ranjan Dash and Surajit Das<br>5. Measuring Microbial Metabolism in Atypical Environments<br>Wendy Stone and Gideon Wolfaardt<br>6. Rock-Inhabiting Fungi: Survival on and Alteration of Solid Air-Exposed Surfaces<br>Nicole Knabe and Anna A. Gorbushina<br>7. Taxonomy of Oral Bacteria<br>Samantha J. Byrne, Catherine A. Butler, Eric C. Reynolds and Stuart G. Dashper<br>8. Aqueous Methods for Extraction/Recovery of Macromolecules From Microorganisms of Atypical Environments: A Focus on Three Phase Partitioning<br>Mohammed Gagaoua<br>9. Microbial Community Composition and Predicted Functional Attributes of Antarctic Lithobionts Using Targeted Next-Generation Sequencing and of Bioinformatics Tools<br>Hyunmin Koo, Joseph Hakim, Casey Morrow, Dale T. Andersen and Asim K. Bej</p> <p>Section 3: Microscopic Methods<br>10. Methods for Collection and Characterization of Samples From Icy Environments<br>Manuel Bedrossian, Jody W. Deming and Jay Nadeau<br>11. Cryo-Electron Microscopy of Extremely Halophilic Microbes<br>Harold Engelhardt and Daniel Bollschweiler</p> <p>Section 4: Organisms<br>12. Methods to Study Magnetotactic Bacteria and Magnetosomes<br>Lei Yan and Weijia Xing<br>13. Molecular Methods to Study Vibrio parahaemolyticus and Vibrio vulnificus From Atypical Environments<br>Indrani Karunasagar, Biswajit Maiti and Ballamoole Krishna Kumar</p>