<p>Preface<br>Helder Maiato and Melina Schuh<br>1. Mitotic live-cell imaging at different timescales<br>Anna-Maria Olziersky, Chris A. Smith, Nigel Burroughs, Andrew D. McAinsh and Patrick Meraldi<br>2. Correlating light microscopy with serial block face scanning electron microscopy to study mitotic spindle architecture<br>Nicholas I. Clarke and Stephen J. Royle<br>3. Quantification of three-dimensional spindle architecture<br>Norbert Lindow, Stefanie Redemann, Florian Brünig, Gunar Fabig, Thomas Müller-Reichert and Steffen Prohaska<br>4. Quantitative live and super-resolution microscopy of mitotic chromosomes<br>Nike Walther and Jan Ellenberg<br>5. Live imaging of cell division in 3D stem-cell organoid cultures<br>Ana C.F. Bolhaqueiro, Richard H. van Jaarsveld, Bas Ponsioen, René M. Overmeer, Hugo J. Snippert and Geert J.P.L. Kops<br>6. Choosing the right microscope to image mitosis in zebrafish embryos: A practical guide<br>Iskra Yanakieva, Marija Matejcic and Caren Norden<br>7. Advanced microscopy methods for bioimaging of mitotic microtubules in plants<br>Petra Vyplelová, Miroslav Ovečka, George Komis and Jozef Šamaj<br>8. Microsurgery and microinjection techniques in mitosis research<br>Charles A. Day, Jessica Hornick, Alyssa Langfald, Christopher Mader and Edward H. Hinchcliffe<br>9. Analyzing the micromechanics of the cell division apparatus<br>Yuta Shimamoto and Tarun M. Kapoor<br>10. Optogenetic reversible knocksideways, laser ablation, and photoactivation on the mitotic spindle in human cells<br>Ana Milas, Mihaela Jagrić, Jelena Martinčić and Iva M. Tolić<br>11. Live imaging of C. elegans oocytes and early embryos<br>Kimberley Laband, Benjamin Lacroix, Frances Edwards, Julie C. Canman and Julien Dumont<br>12. Combining microscopy and biochemistry to study meiotic spindle assembly in Drosophila oocytes<br>Pierre Romé and Hiroyuki Ohkura<br>13. Methods to study meiosis in insect spermatocytes<br>James R. LaFountain, Jr and Christopher S. Cohan<br>14. Live-cell microscopy of meiosis in spermatocytes<br>Hiroki Shibuya and Yoshinori Watanabe<br>15. Live imaging of cell division in preimplantation mouse embryos using inverted light-sheet microscopy<br>Judith Reichmann, Manuel Eguren, Yu Lin, Isabell Schneider and Jan Ellenberg<br>16. Correlated light and electron microscopy of cell division in large marine oocytes, eggs, and embryos<br>Mariia Burdyniuk, Natalia Wesolowska, Michal Fleszar, Matthia A. Karreman, Pedro Machado, Joana Borrego-Pinto, Bernhard Ruthensteiner, Yannick Schwab and Péter Lénárt<br>17. A microscopy-based approach for studying meiosis in live and fixed human oocytes<br>Agata P. Zielinska and Melina Schuh<br>18. Superresolution imaging of the synaptonemal complex<br>Katharina Schücker, Markus Sauer and Ricardo Benavente<br>19. Cryoelectron tomography of SYCP3 fibres under native conditions<br>Daniel Bollschweiler, Laura Radu and Luca Pellegrini</p>