<p>1. Empirical Valence Bond Simulations of Organophosphate Hydrolysis: Theory and Practice<br>Miha Purg and Shina Caroline Lynn Kamerlin<br>2. Analysis of Phosphoryl-Transfer Enzymes With QM/MM Free Energy Simulations<br>Daniel Roston, Xiya Lu, Dong Fang, Darren Demapan and Qiang Cui<br>3. Defining Dynamics of Membrane-Bound Pyrophosphatases by Experimental and Computational Single-Molecule FRET<br>Steven P.D. Harborne, Jannik Strauss, Ainoleena Turku, Matthew A. Watson, Roman Tuma, Sarah A. Harris and Adrian Goldman<br>4. A Simple Strategy to Determine the Dependence of Membrane-Bound Pyrophosphatases on K<SUP>+</SUP> as a Cofactor<br>Jannik Strauss, Craig Wilkinson, Keni Vidilaseris, Steven P.D. Harborne and Adrian Goldman<br>5. Crystallization of Liganded Phosphatases in the HAD Superfamily <br>Christine Harvey, Katherine O’Toole and Karen N. Allen<br>6. Structure, Mechanism, and Substrate Profiles of the Trinuclear Metallophosphatases From the Amidohydrolase Superfamily<br>Swapnil V. Ghodge and Frank M. Raushel<br>7. Affinities and Comparisons of Enzyme States by Principal Component Analysis of NMR Spectra, Automated Using TREND Software<br>Jia Xu and Steven R. Van Doren<br>8. Assessment and Impacts of Phosphorylation on Protein Flexibility of the α-D-Phosphohexomutases<br>Kyle M. Stiers and Lesa J. Beamer<br>9. Chemical Tools for Studying the Impact of cis/trans Prolyl Isomerization on Signaling: A Case Study on RNA Polymerase II Phosphatase Activity and Specificity<br>Nathaniel Tate Burkholder, Brenda Medellin, Seema Irani, Wendy Matthews, Scott A. Showalter and Yan Jessie Zhang<br>10. Quantitative Analysis of Intracellular Response Regulator Phosphatase Activity of Histidine Kinases<br>Rong Gao and Ann M. Stock<br>11. Measuring the Activities of Two-Component Regulatory System Phosphatases<br>Robert B. Bourret and Ruth E. Silversmith<br>12. Assembly of PGAM5 Into Multimeric Complexes Provides a Mechanism for Allosteric Regulation of Phosphatase Activity<br>Peter Tipton, Tong Su and Mark Hannink<br>13. Purification of Lipin and Measurement of Phosphatidic Acid Phosphatase Activity From Liposomes<br>Mitchell E. Granade and Thurl E. Harris<br>14. Combining SAMDI Mass Spectrometry and Peptide Arrays to Profile Phosphatase Activities<br>Lindsey C. Szymczak, Che-Fan Huang, Eric J. Berns and Milan Mrksich<br>15. Protein Chemical Approaches to Understanding PTEN Lipid Phosphatase Regulation<br>Daniel R. Dempsey and Philip A. Cole</p>