DNA Repair Enzymes: Structure, Biophysics, and Mechanism

<p>1. MacroBac: New Technologies for Robust and Efficient Large-Scale Production of Recombinant Multiprotein Complexes<br>Scott D. Gradia, Justin P. Ishida, Miaw-Sheue Tsai, Chris Jeans, John A. Tainer and Jill O. Fuss<br>2. Production and Assay of Recombinant Multisubunit Chromatin Remodeling Complexes<br>David M. Rees, Oliver Willhoft, Chia-Liang Lin, Rohan Bythell-Douglas and Dale B. Wigley<br>3. Analysis of Functional Dynamics of Modular Multidomain Proteins by SAXS and NMR<br>Matthew K. Thompson, Aaron C. Ehlinger and Walter J. Chazin<br>4. Use of Single-Cysteine Variants for Trapping Transient States in DNA Mismatch Repair<br>Peter Friedhoff, Laura Manelyte, Luis Giron-Monzon, Ines Winkler, Flora Groothuizen and Titia K. Sixma<br>5. Expression and Structural Analyses of Human DNA Polymerase θ (POLQ)<br>Andrew W. Malaby, Sara K. Martin, Richard D. Wood and Sylvie Doublié<br>6. Structural Studies of RNases H2 as an Example of Crystal Structure Determination of Protein-Nucleic Acid Complexes<br>Małgorzata Figiel and Marcin Nowotny<br>7. DNA-PKcs, Allostery, and DNA Double-Strand Break Repair: Defining the Structure and Setting the Stage<br>Dimitri Y. Chirgadze, David B. Ascher, Tom L. Blundell and Bancinyane L. Sibanda<br>8. Single-Particle Electron Microscopy Analysis of DNA Repair Complexes<br>Marta Sawicka, Ricardo Aramayo, Rafael Ayala, Robert Glyde and Xiaodong Zhang<br>9. Using Atomic Force Microscopy to Characterize the Conformational Properties of Proteins and Protein-DNA Complexes that Carry Out DNA Repair<br>Sharonda LeBlanc, Hunter Wilkins, Zimeng Li, Parminder Kaur, Hong Wang and Dorothy A. Erie<br>10. Single-Molecule Methods for Nucleotide Excision Repair: Building a System to Watch Repair in Real Time<br>Muwen Kong, Emily C. Beckwitt, Luke Springall, Neil M. Kad and Bennett Van Houten<br>11. Next-Generation DNA Curtains for Single-Molecule Studies of Homologous Recombination<br>Michael M. Soniat, Logan R. Myler, Jeffrey M. Schaub, Yoori Kim, Ignacio F. Gallardo and Ilya J. Finkelstein<br>12. Detection of Reaction Intermediates in Mg2+-Dependent DNA Synthesis and RNA Degradation by Time-Resolved X-Ray Crystallography<br>Nadine Samara, Yang Gao, Jinjun Wu and Wei Yang<br>13. Analyzing the Catalytic Activities and Interactions of Eukaryotic Translesion Synthesis Polymerases<br>Kyle T. Powers and M.T. Washington<br>14. Kinetic Methods for Studying DNA Glycosylases Functioning in Base Excision Repair<br>Christopher T. Coey and Alexander C. Drohat<br>15. Transient Kinetic Methods for Mechanistic Characterization of DNA Binding and Nucleotide Flipping<br>Jenna M. Hendershot and Patrick J. O’Brien<br>16. What Combined Measurements from Structures and Imaging Tell Us About DNA Damage Responses<br>Chris A. Brosey, Zamal Ahmed, Susan P. Lees-Miller and John A. Tainer</p>