Enzymology at the Membrane Interface: Interfacial Enzymology and Protein-Membrane Binding

Specificaties
Gebonden, blz. | Engels
Elsevier Science | e druk, 2017
ISBN13: 9780128094198
Rubricering
Elsevier Science e druk, 2017 9780128094198
Onderdeel van serie Methods in Enzymology
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Samenvatting

Enzymology at the Membrane Interface, the latest volume in the Methods in Enzymology series, covers a subset of enzymes that work in the environment of the biological cell membrane. This field, called interfacial enzymology, involves a special series of experimental approaches for the isolation and study of these enzymes.

Specificaties

ISBN13:9780128094198
Taal:Engels
Bindwijze:Gebonden

Inhoudsopgave

<p>1. A High-Throughput Flurometric Assay for Lipid-Protein Binding<br>Wonhwa Cho<br>2. Fluorescence-based in situ quantitative imaging for cellular lipids<br>Wonhwa Cho<br>3. Recombinant Expression of human and mouse groups I, II, III, V, V and XII Secreted Phospholipases A2<br>Michael H. Gelb and Gerard Lambeau <br>4. Vesicle-based assay of secreted phospholipase A2 enzymatic activity for high throughput screening of compound libraries<br>Michael H. Gelb<br>5. Cellular Assays for Evaluating Calcium-Dependent Translocation of cPLA2a to Membrane<br>Christina C. Leslie<br>6. Secreted Phospholipase A2 Specificity on Natural Membrane Phospholipids<br>Makoto Murakami<br>7. Analyses of Calcium-Independent Phospholipase A2beta (iPLA2β) in Biological Systems<br>Sasanka Ramanadham<br>8. Using hydrogen deuterium exchange mass spectrometry to examine protein membrane interactions<br>John E. Burke<br>9. Lipid-binding C2 domains mediate a diverse class of enzymatic functions"<br>Robert V. Stahelin<br>10. Interfacial Enzymes: Membrane Binding, Orientation, Membrane Insertion, and Activity<br>Suren Tatulian<br>11. Enzymology at the Membrane Interface<br>Dan M. Raben<br>12. Studying gastric lipase adsorption onto phospholipid monolayers by surface tensiometry, ellipsometry and atomic force microscopy<br>Frédéric Carrière<br>13. Probing conformational changes and interfacial recognition site of lipases with surfactants and inhibitors<br>Frédéric Carrière<br>14. Measuring Phospholipase D enzymatic activity through biochemical and imaging methods<br>Michael A. Frohman<br>15. Pld Rotein-Protein Interaction with Intracellular Signaling Molecules, Modulation by Mitogen Pa and Pld’s Gef Activity<br>Julian G. Cambronero<br>16. Phosphatidic Acid Regulation of PIPKI<br>Guangwei Du</p>
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        Enzymology at the Membrane Interface: Interfacial Enzymology and Protein-Membrane Binding