Calorimetry

Specificaties
Gebonden, blz. | Engels
Elsevier Science | e druk, 2016
ISBN13: 9780128029060
Rubricering
Elsevier Science e druk, 2016 9780128029060
Onderdeel van serie Methods in Enzymology
€ 196,00
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Samenvatting

Calorimetry, the latest volume in the Methods in Enzymology series continues the legacy of this premier serial with quality chapters authored by leaders in the field. Calorimetry is a highly technical experiment and it is easy for new practioners to get fooled into interpreting artifacts as real experimental results. This volume will guide readers to get the most out of their precious biological samples and includes topics on specific protocols for the types of studies being conducted as well as tips to improve the data collection. Most importantly, the chapters will also help to identify pitfalls that need to be avoided to ensure that the highest quality results are obtained.

Specificaties

ISBN13:9780128029060
Taal:Engels
Bindwijze:Gebonden

Inhoudsopgave

1. Isothermal Titration Calorimetry Measurements of Metal Ions Binding to Proteins<br>Colette F. Quinn ,Margaret C. Carpenter, Molly L. Croteau and Dean E. Wilcox<br>2. Assessing Coupled Protein Folding and Binding through Temperature- Dependent Isothermal Titration Calorimetry<br>Debashish Sahu, Monique Bastidas, Chad W. Lawrence, William G. Noid,and Scott A. Showalter<br>3. Fragment-Based Screening for Enzyme Inhibitors Using Calorimetry<br>Michael I. Recht, Vicki Nienaber and Francisco E. Torres<br>4. Measuring Multivalent Binding Interactions by Isothermal Titration Calorimetry<br>Tarun K. Dam,  Melanie L Talaga, Ni Fan and Curtis F. Brewer<br>5. Calorimetric and Spectroscopic Analysis of the Thermal Stability of Short Duplex DNA-Containing Sugar and Base-Modified Nucleotides<br>Kareem Fakhfakh, Curtis B. Hughesman, A. Louise Creagh, Vincent Kao and  Charles Haynes<br>6. Calorimetric Quantification of Cyclodextrin-Mediated Detergent Extraction for Membrane-Protein Reconstitution<br>Martin Textor and Sandro Keller<br>7. Extending ITC to Kinetics with KinITC<br>Philippe Dumas, Eric Ennifar, Cyrielle Da Veiga, Guillaume Bec, William Palau, Carmelo Di Primo,Angel Piñeiro, Juan Sabin, Eva Muñoz and Javier Rial<br>8. Measuring the Kinetics of Molecular Association by Isothermal Titration Calorimetry <br>Kirk A. Vander Meulen, Scott Horowitz, Raymond C. Trievel, and Samuel E. Butcher<br>9. Isothermal Titration Calorimetry to Characterize Enzymatic Reactions<br>Luca Mazzei, Stefano Ciurli and Barbara Zambelli<br>10. Avoiding Buffer Interference in Itc Experiments: A Case Study from the Analysis of  Entropy Driven Reactions of Glucose-6-Phosphate Dehydrogenase<br>M. Lucia Bianconi<br>11. ITC Methods for Assessing Buffer/Protein Interactions from the Perturbation of  Steady-State Kinetics: A Reactivity Study of Homoprotocatechuate 2,3-Dioxygenase<br>Kate L. Henderson, Delta K. Boyles, Vu H. Le, Edwin A. Lewis and Joseph P.Emerson<br>12. Modern Analysis of Protein Folding by Differential Scanning Calorimetry<br>Beatriz Ibarra-Molero, Athi N. Naganathan, Jose M. Sanchez-Ruiz and Victor Muñoz<br>13. A Guide to Differential Scanning Calorimetry of Membrane and Soluble Proteins in Detergents<br>Zhengrong Yang and Christie G. Brouillette<br>14. Orthogonal Methods for Characterizing the Unfolding of Therapeutic Monoclonal  Antibodies: Differential Scanning Calorimetry, Isothermal Chemical Denaturation and  Intrinsic Fluorescence with Concomitant Static Light Scattering<br>Deniz B. Temel, Pavel Landsman and Mark L. Brader<br>15. IATC, DSC, and PPC Analysis of Reversible and Multi-State Structural Transition of Cytochrome c<br>Shun-ichi Kidokoro and Shigeyoshi Nakamura<br>16. The Complementarity of the Loop to the Stem in DNA Pseudoknots Gives Rise to Local TAT Base-Triplets<br>Calliste Reiling-Steffensmeier and Luis A. Marky<br>17. A High-Throughput Biological Calorimetry Core: Steps to Startup, Run, and Maintain a Multi-User Facility<br>Neela H. Yennawar, Julia A. Fecko, Scott A. Showalter and Philip C. Bevilacqua
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